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26 November 2013

The preliminary results for our Dalea purpurea experiment are in! Melisa, our Lake Forest College student, came for the final installment of her mini-internship yesterday where we analyzed the results of the full pod counts. Just as a reminder, these pods originally came from 120 D. purpurea plants in 6 remnants (Lf, Spp, Sgc, Woodys, Rrx, and On27) and we hypothesized that the sites with the largest populations (Spp and Lf) would also have the highest seed set. After looking at the xray images (see a photo example below), we compared average seed set, as measured by full pod counts, across the 6 remnants and found that On27 and Woody’s had the highest seed set. There was enough variability between sites that our results were significant (meaning that site matters when looking at average seed set of Dalea purpurea populations). Obviously sheer remnant size is not the only factor influencing seed set in these populations since On27 and Woody’s especially, are relatively small. More investigation to come!

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Our volunteers have been hard at work cleaning and counting the past few weeks. After recovering two “missing” boxes of achenes from 2011, Susie finished up all the weighing that needed to get done and we were able to organized the 2011 achenes in hopes of getting them placed in long term storage later this year. Here’s an action shot of Susie and Susan cleaning!

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I’m in the midst of searching for background literature on inbreeding and phenology as I’m hoping to asses whether mating system influences flowering time using Team Echinacea phenology data in the INB gardens. I’ll keep you all updated as these results come in.

Stuart’s off to Minnesota and I’m off to Rhode Island today to celebrate Thanksgiving. We hope you all have a wonderful holiday!

14 November 2013

Last week the volunteers began cleaning heads from this years harvest. It was a productive week; we cleaned ~60 heads.

This week and last week students from Lake Forest College came to the botanic gardens to begin mini-internships. They will be coming two more times in the next two weeks to assist various labs in projects. For our project, we’re looking at seeds of Dalea purpurea we collected from six different remnants this fall (SPP, Woodys, RRX, On27, SGC, and LF–120 plants total). We’re planning on taking a sub-sample of seeds from every plant and x-raying them to see if seed set varies in different remnants. The past two weeks we’ve had Melissa (our student) randomize the seeds to take our sub-sample. We’ll keep you posted on our findings!

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Our hope is eventually to plant these Dalea seeds among our Echinacea in the new common garden (south field). Who knows, this could be the beginning of The Dalea Project.

In other news we’ve already got volunteers underway with counting full achenes in our X-ray images (see flog post below for example) from the qGen2 crossing experiment and soon we’ll be able to analyze these data and see how seed set varies among different crosses.

new x-ray images

We took some high-quality images of Echinacea achenes for our q2 experiment this fall; an example is below. Notice how easy it is to distinguish empty achenes from those with embryos. By darkening the room and removing the opaque film, we were able to use lower levels of xrays for a shorter duration than we have previously. This plate was exposed to 12kV x-rays for 4s. We used long, thin glassine envelopes to facilitate counting. Notice also that the laser-printed labels reveal the packet IDs.

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X-ray image of 30 packets of achenes from
Echinacea angustifolia. Click on thumbnail to enlarge.

A Summary of Recent Evens

Well it has been quite a while since our last update and a lot has happened in the last couple weeks. I don’t think I’ll be able to recall everything that we did to wrap up the field season, but here’s a rough summary of a last few weeks.

We finished harvesting all the heads for this year on October 18 which I think is probably one of the latest harvest dates ever for Team Echinacea! The volunteers at the botanic gardens have now begun inventory on the ~2300 heads from this season.

We burned the new common garden site! The weather/wind direction looked good and so on October 9th Stuart, Dwight, Ilse and I went out and set afire the south field. It was both Ilse’s and my first burning experience and I think both of us found it be hotter and smokier than we were expecting. The burn took roughly 3 hours and was a little patchy in places but overall a huge success and made clearing the rest of the plot and planting a whole lot easier.

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The next step after the burn for the qGen2 crossing experiment was to clear all the trees, saplings, stumps, etc from this new field site and apply another round of herbicide to ensure our prairie stays prairie in the future. After this was completed, Ilse and I then set out to stake (with the GPS equipment) a planting grid. Unlike other common gardens, planting in the south field did not take place in consecutive rows. Instead, we planted along “random” rows in the field in order to ensure that more plantings can take place in future years and so that we can compare different plantings across the field site without worrying about any small scale effects of location.

Prior to planting, however, we x-rayed the achenes down at the Chicago Botanic Gardens. We spent roughly two full days scanning and packaging achenes into glassine envelopes which we were able to subject to very low doses of x-ray and still decipher how many full/empty achenes there were. Unfortunately, some crosses did not yield any full achenes (perhaps these were incompatible?) but the silver lining was that we were able to look at the x-rays, pick out the crosses with only empty achenes, and exclude them from the planting portion of the experiment.

Planting got delayed a bit because of wet and cold weather, but we finally put the seeds on the ground this past Thursday and Friday (Oct 24-25). Ruth Shaw and Katherine Muller came to help out on Friday. We planted achenes from each cross along one meter segments. Planting went smoothly (it was 50 degrees on Friday!) except it was very windy. Even though Echinacea is not normally considered to be wind dispersed, we had be to extremely cautious when planting since large gusts of wind were frequent and could easily blow away the achenes.

And with the gQen2 crossing experiments seeds on the ground, the field season has finally come to a close. Ilse and I departed from Kensington, our home for nearly 5 months, this past weekend and went our separate ways. I came down to Chicago and started working at the botanic gardens this past Monday where I’ll be managing Stuart’s lab until June.