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Planting Day Details

We had a very quick and efficient planting last week. Stuart and I drove up to MN on Thursday morning, picking up Katherine Muller on the way in St. Paul. We arrived in Kensington on Thursday evening, and immediately got to work. We broadcasted our remaining little bluestem and side oats gramma grass seeds in the South Field (where we planted our qGen2 achenes this fall, aka CG-8). We walked E/W throughout the plot and each broadcast seed over a 5m area. We also broadcast seed around the edge of the plot. Here’s an inventory of how much we broadcast from each of the collection sites:

Peninsula – 1 quart
Back Hill – 1 quart
LCW – 2 quarts
CR-15 – 1 quart
Tower – 1 quart
Krus – 1 quart
RRX- 1 oz
CG1 – 1 gallon (24 Sept) + 1 gallon (30 Sept) + 2.5 gallons (2 Oct).

We then headed to Hegg Lake to scout out a planting site for the sprouts. We settled on a spot near the parking area, to the west of Amy’s, Caroline’s, and the recruitment plots. The plot is 12m x 30 m with 13 rows and 60 positions. There is a meter spacing between rows and half a meter between positions.

We returned the next morning and began planting by 9:30am. With three of us we were very efficient and finished our last row by 3:30pm. We we lucky to have beautiful weather, it was sunny all day with temperatures in the low-mid 70s and a consistently strong breeze. All positions in the plot were plant-able. Here’s a diagram of the plot:

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Empty circles indicate positions where a sprout was planted and black dots indicate positions where there are nails. Plants begin at position 0.5 in every row. Row 41 is the most western row, and position 0 is closest to the road (and north). There are empty positions at the ends of rows 46 and 48. There is a bent over flag at each of the 4 corners of the plot.

I’m excited to have the sprouts in the ground and to have participated in my first planting. Fingers crossed we have high survival!

Wrapping up

Spring has caught up with us quickly and now it’s almost summer. We’re getting prepared for a new field season and finishing up some of our lab projects.

Our sprouts are in the ground! It’s been quite the germination process. We had an overall germination rate of 97% for all sprouts with a E. pallida mother and an overall germination rate of 87% for sprouts with an E. angustifolia mother. That’s a lot higher than we expected! Consequently, our plug trays were very full.

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Germination also spanned more than a month, with our first radicals emerging on April 7th and our last emerging on May 8th. We measured all sprouts when they were 21 days old and decided to take leaf tissue samples when they were 28 days old. The tallest sprout was a whopping 7.4 cm. We took leaf samples primarily to be able to analyze the sprouts’ DNA and perform paternity tests since we also have tissue from their parents. This will be a project for a future date.

Alex, our Lake Forest College intern, has finished up his analysis on the heads in the aphid addition/exclusion experiment and is working on a report that we will post in the coming weeks.

We had our annual Volunteer Appreciation Potluck last week. Gia came and presented some of her ant data and Gretel and I talked about some of our recent research. We’re so lucky for all the help we receive from our volunteers, we really couldn’t do our research without them. Although there are many steps in Echinacea workflow process (cleaning, scanning, counting, randomizing, and weighing/x-raying) we decided to recognize one of our volunteers, Lois, as the “Achene Queen.” Since we’ve transferred all our counting to an online database, Lois has counted over 350,000 achenes!

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