In summers 2018 and 2019, Amy mapped and collected leaf tissue from all individuals in the study areas and harvested heads from a subset of Echinacea individuals at populations in the NW corner of the Echinacea Project study area (populations: ALF, EELR, KJ, NWLF, GC, SGC, NGC, KJ, NNWLF) to map pollen movement (see Reproductive Fitness in Remnants). Amy analyzed patterns of gene flow, by assessing how individuals’ location and timing of flowering influence their reproductive success and distance of pollen movement. The now four-year-old seedlings are planted in p10.
There was no fieldwork conducted this summer but, in exciting news, Amy used the seed set data from the gene flow in remnants experiment in her dissertation chapter, “Variation in reproductive fitness among individual plants depends on the spatial proximity of prospective mates and the timing of their reproduction”. Amy defended her dissertation in May, and this chapter is currently in preparation for publication!
Amy visited the station a few times this summer! Cupcakes in the field to celebrate a successful dissertation defense!
Start year: 2018
Location: Roadsides, railroad rights of way, and nature preserves in and around Solem Township, MN
As part of the Echinacea Project’s long-term efforts to monitor reproductive fitness in the remnant populations, we harvested 124 Echinacea seed heads from 16 remnants during summer 2023. All sites burned in 2023 were included in remnant harvest selection and we also prioritized sites with small population sizes. We randomly selected heads from each population at the sites we visited.
Harvesting seed heads and quantifying seed set can help us to better understand how the spatial location and flowering phenology of Echinacea contribute to reproductive fitness. However, this year, we did not conduct field work for phenology, so we were less interested in relating remnant harvest to phenology and spatial mating opportunities. Our primary focus was to examine fire’s affects on reproduction in different sized populations. We narrowed our remnant harvest efforts to small sites where extra data would be helpful in answering questions about how fire influences mating opportunities and seed set across different populations ranging in size. The heads harvested in 2023 are currently in the CBG lab. We have entered the harvest data, and the heads are awaiting inventory!
Echinacea harvest in action!
In order to ensure that we are not disrupting remnant populations, we return achenes to their maternal plants in a way that mimics natural dispersal after they have been processed in the lab. On a recent trip to Minnesota, we visited many small sites, where this process is essential as well as a few bigger ones.
Start year: 1996
Location: Roadsides, railroad rights of way, and nature preserves in and around Solem Township, MN
Data collected: The verified harvest list and legend is located here: ~/Dropbox/remData/130_harvestSeedSet/harvest2023/2023remHarvestDataEntry/2023remEaHarvestDE/remEaHarvestVerified
Samples collected: ~124 seed heads were collected and are currently at the Chicago Botanic Garden lab awaiting inventory.
Products:
We will compile seed set data from 2023 into a dataset with seed set data from previous years.
Padmini, a student from Carleton College, used some of the remnant data for her externship project in winter 2022.
Jared, Amy, and Stuart’s paper “Habitat fragmentation decouples fire-stimulated flowering from plant reproductive fitness” was published in PNAS. Find links and more information here
You can read more about the reproductive fitness in remnants experiment, as well as links to prior flog entries about this experiment, on the background page for this experiment.
This recruitment experiment was established in 2000 to quantify seedling emergence and juvenile survival of Echinacea angustifolia during reintroduction to sites with varying land-use history and burn schedules. Before 2014, we collected detailed data on each plant in the plots. Since 2014, Team Echinacea has censused each plot yearly to collect demographic data for every flowering plant.
In 2023, Team Echinacea visited 7 recruitment plots and searched for 163 Echinacea angustifolia plants that had flowered previously. Across the 7 plots, we found 118 basal plants and 46 flowering plants, and we were unable to find 16 plants. Of the flowering plants, 11 plants flowered for the first time in 2023. For each flowering plant, the team collected demographic data (number of rosettes and flowering heads) and shot a GPS point at the exact location of the plant. There was at least one flowering Echinacea plant at 6 of the 7 recruitment plots where plants had flowered previously, but we did not find any flowering plants at one site, rhp.
There were also two sites, rhr and rhw, that we have no 2023 demographic information for. We did not visit these sites for total demo censusing, and it’s possible that we never visited them over the summer. Alternatively, we may have visited them and found no flowering plants. Rhw was present on “demopup” (a list of demo sites for mopping-up any flowering plants that may have been missed), so there were likely no flowering plants there this year. Rhr was not on the demopup list. Bizarre! Stay tuned for any updates we may uncover.
Abby, Alexa, and Blaire search for plants at rkw, a recruitment plot located at Kensington WMA. We found 4 flowering plants and 4 basal plants at rkw this year.
This year, we used “demopup” to track progress as we revisted sites where we searched for and demo’ed flowering plants. Rhw made it on the list, but no such luck for rhr.
Start year: Plantings in 2000-2002
Location: Seven study plots on state land with different land use histories: old-field and restored grassland
You can read more about the fire in recruitment experiment, as well as links to prior flog entries about this experiment, on the background page for this experiment.
Since 1995, the Echinacea Project has been mapping and collecting demographic information on Echinacea angustifolia to generate long-term records detailing individual fitness in prairie remnants. In summer 2023, Team Echinacea visited 42 prairie remnants to search at 2443 locations where adult Echinacea plants had been previously mapped. We call this “total demo.” At small sites, the team took records for all adult plants found at a site (no change in total demo protocol from previous years). At larger sites, we scaled down and visited a subset of adult plants. Burning led to high flowering rates and lots of newly flowering plants entering the census, which stressed our system for total demo. This year we did not visit plants that were “not present” for the past 3+ years and we also capped total demo points at 100 per site. For example, at Landfill, we searched at 100 locations at Landfill East and 100 Locations at Landfill West.
We used stake files on our high-precision GPS units to stake to each Echinacea plant in our total demo visit list, where we recorded flowering status, number of flowering heads, number of rosettes, and near neighbors of the plant on handheld data collectors (visors).
In addition to total demo, we searched and took records for all flowering plants in our remnant sites. For flowering demo, we visited 50 sites. In summer 2023, we took 5,601 demographic records in prairie remnants (demo) and 1929 GPS records (surv). We saw a much lower flowering year following 2022, with ~1586 flowering plants total. At Landfill, there were ~262 flowering plants and at Loeffler’s corner, there were 285.
Alexa and Jak step carefully through Kjs as they search for flowering Echinacea plants. 63 plants flowered at Kjs in 2023!
We also took demo and surv data as part of our Pollen and Nectar project where we will compare characteristics of pollen and nectar in burned vs. unburned prairies. We collected demographic data at a subset of plants at several sites where we have never done demo or surv. We put out our first tags at hulze, hulzw, torges, torgen, hutche, hutchw, and koons.
This year, we put out 528 new tags which started at 28001. Two 29000 tags were accidentally created and placed in Landfill East, but those were quickly removed during our demo rechecks following completion of flowering demo and total demo.
Lindsey visits an 18 headed Echinacea plant at Steven’s Approach. This plant produced the most heads of any this year.
After revisiting a final round of recheck plants during a trip to Minnesota in mid-November, we are just getting ready to move data from aiisummer2023 into demap.
Start year: 1995
Location: Remnant prairie populations of the purple coneflower, Echinacea angustifolia, in Douglas County, MN. Sites are located between roadsides and fields, in railroad margins, on private land, and in protected natural areas.
Total demo: Bill Thom’s Gate, Common Garden, Dog, East of Town Hall, Golf Course, Martinson’s Approach, Near Pallida, Nessman, North of Golf Course, South of Golf Course, Sign, Town Hall, Tower, Transplant Plot, West of Aanenson, Woody’s, Yellow Orchid Hill, plus the recruitment plots REL, RHE, RHP, RHS, RHX, RKE, RKW
Annual sample: Aanenson, Around Landfill, East Elk Lake Road, East Riley, KJ’s, Krusemarks, Loeffler’s Corner, Landfill, North of Railroad Crossing, Northwest of Landfill and North of Northwest of Landfill (lumped), On 27, Riley, Railroad Crossing, Steven’s Approach, Staffanson Prairie
Plant status (can’t find, basal, dead this year’s leaves, dead last year’s leaves, flowering), number of rosettes, nearest neighbors, and head count, if flowering
All GPS files are found here: Dropbox/geospatialDataBackup2023
All demo and surv records are stored in the aiisummer2023 repo
The most recent copies of allDemoDemo.RData and allSurv.RData can be accessed at Dropbox/demapSupplements/demapInputFiles
Samples or specimens collected: NA
Products:
Amy Dykstra’s dissertation included matrix projection modeling using demographic data
The “demap” project is a long-term dataset that combines phenological, spatial and demographic data for remnant plants
You can read more about the demographic census in the remnants, as well as links to prior flog entries about this experiment, on the background page for this experiment.
For many, the end of the year brings thoughts of final exams, final reports, and final projects. Here at the Echinacea Project, we don’t believe in final anything, but we do believe in updates! Last week, two of our Lake Forest College interns, Sophia and Olivia, shared project updates with the lab: a culmination of their semester in the lab.
Olivia shared results from her study on plant and reproductive health indicators (more here)
Olivia and Sophia present research updates at lab meeting!
Sophia shared a poster with the lab and also with attendees of a Lake Forest College research symposium! Sophia’s research focuses on the effects of pollen limitation on life history fitness in Echinacea. (More info to come!)
Sophia Presents a poster at Lake Forest College’s Glassman Symposium.
Members of team Echinacea went back to Minnesota to do some final field work and winterization of our field station. We had fantastic weather for field work but northerly winds brought chilly temps by the end of the week and reminded us that winter is near . Here are some updates from all we got done these past few days!
Seed Add
We added seeds to 84 experimental transects at 36 sites for our seed addition experiment, which measures the effects of prescribed fire on seedling germination and emergence in Echinacea. We made quick work and were able to get this done in just 1 day plus an extra morning!
Here is Wyatt sprinkling E. angustifolia achenes along our seed addition transects.
Prescribed Burning and Broadcasting Seed
We got two solid days of good burn weather– more than any of us predicted! Over this window we burned six units, including the production plot, a few oak litter areas, Jean’s prairie garden, and the southeast hill. After all this burning, we broadcasted native seed collected by the team this summer. We’re excited to see what happens in these units next year!
Jean’s prairie garden burned in ~5 minutes
Jared gets our 1st burn started
Wyatt broadcasts seed into this freshly burned unit
Return Achenes to Remnants
Every summer we harvest a subset of Echinacea heads from remnant sites to assess fitness. In order to ensure that we are not disrupting these populations, we later return the achenes to their maternal plants in a way that mimics natural dispersal. On this trip, we visited many small sites, where this process is essential as well as a few bigger ones.
Woody Encroachment Pilot
I tested out methods for my research project looking at the effects of Sumac encroachment on Echinacea fitness and mortality. I collected height and spatial location information on Sumac plants in order to quantify encroachment. In an encroached site like Tower, this process was no walk in the park…
Here I am staking to one of my random points
Post- Summer Sleuthing
When mistakes during summer data collection happen, it takes some investigating to figure out where we went wrong. On this trip, we revisited plants with issues from demographic data collection AND we found a P02 plant that was never harvested during the summer!
It’s autumn at Chicago Botanic Garden! Geese fly south overhead, the trees are ablaze, goldenrod has gone to seed, and there was even a dusting of snow on the ground this morning (which made for a treacherous bike ride along the Green Bay Trail).
But there are also many other signs within the Population Biology lab that will tell you fall is in the air. All of our heads from 2023 are done drying and we have just begun to inventory our harvest.
Here is Wyatt, pulling out the very first bag for 2023 inventory.
How else do we know it’s fall? We are gathering seeds and datasheets in preparation for a final return to Minnesota. We put these seeds out in experimental plots/transects or remnants “as late as possible, but not to late”. Ian is entering the last of the data from pollen and nectar collection this summer and it feels like forever ago. And when 4pm hits, the sun shines though the atrium and fills our lab with natural light. This doesn’t happen very often, but it’s a treat when it does.
Maybe it is all these tell-tale signs from the Echinacea Project that told the wild geese- “It was time to go”.
Here in the lab at the Garden I’ve settled, In demap and on ACE, oh boy, have I meddled. New faces I’ve seen, Not to mention achenes And all through the trails I have pedaled.
The foliage here is quite a delight, And from the Metra the city looks bright. But Minnesota’s fire Is my heart’s true desire, The prescription to better my plight.
This past week, Team Echinacea volunteer, Allen, hit a milestone of 1 million achenes counted. That’s a lot of achenes! This achievement has led to his promotion to Chief Echinacea Officer, a highly esteemed role. Thanks for all your hard work, Allen!
A top hat is a must for any CEO Mid-morning achene cupcake snack
