It feels like forever ago that our summer team of plant demographers were taking demo and surv records on thousands of flowering and non-flowering Echinacea plants in the field! But for me, demo and surv work is still front and center, and it gets more exciting every day!
A few weeks ago, I cleaned up the 2023 data that Stuart and Jared kindly loaded into demap. Now, it is time to reconcile entries within years and between years. There is a lot going on in the demap repository where this happens, but luckily, former members of Team Echinacea wrote great protocols and annotated their scripts thoroughly.
On Friday I wrote my first ever “ICE” record (informed census evaluation) for an entry at Kjs. There will be many more to come as I solve little mysteries from data collection. Hopefully soon we will have successfully incorporated 2023 demographic data into our long-term database. Stay tuned!
A very official certification of my first ICE record.
Most of the hundreds (or thousands!) of Echinacea heads we harvest every year are from our common garden experimental plots. But not all of them! We also harvest heads from local prairie remnants to learn about isolated natural Echinacea populations of different sizes. This year, we harvested 125 heads from the remnants, and they’ve begun their journey through our ACE process at CBG!
Next step next time?
The remnant heads are almost all through the first batch in our process, cleaning. By the end of the ACE process, we’ll be able to quantify multiple components of fitness, such as achene count and seed set, for each individual. But for now, one thing at a time!
The Echinacea Project is assembling an enthusiastic team of undergraduates, recent graduates, graduate students, high schoolers and teachers to engage in prairie research during the summer of 2024! Are you an aspiring ecologist, conservation biologist, or evolutionary biologist? Spend time in western Minnesota’s prairies and gain research experience in plant population biology, evolution and quantitative genetics, pollination biology, and plant-insect interactions!
Members of Team Echinacea 2023 measure Echinacea plants in an experimental plot
We strive to create an inclusive, collaborative, stimulating, positive, fun, and productive environment for all regardless of race, ethnicity, gender, age, sexual orientation, and economic background. We welcome and encourage individuals from groups historically excluded from sciences and conservation. If you are interested in learning about and contributing to science and conservation, please join our team. We are committed to recruiting, training, and supporting individuals interested in science, education, and conservation from diverse backgrounds.
This week, Abby and I designed a poster, packed our bags, and headed to Minnesota to attend a science expo at Morris Area High School. The event was organized by Britney House (Team Echinacea RET 2022) and provided students of all grade levels an opportunity to explore different career paths and opportunities in science. There were folks with fancy robots and all the newest engineering tech in attendance, but we weren’t the only environmental sciencey group there; we were happy to see the MN DNR, USDA NRCS, and others in attendance.
Abby shows off our poster before the expo begins.
Our goals at the event were to inform people of our work in the area, get kids interested in conservation, and advertise our RET and RAHSS opportunities to local high school students and teachers. We got the opportunity to talk to lots of different people, from kids to community members to other exhibitors!
Wyatt sows seeds of conservation-mindedness in the youth. Future Echinacea Project members?
Many thanks to Britney and the rest of the crew at Morris for organizing such a great experience for students, the community, and orgs like us alike. After the event, we revisited some of our favorite spots around town before heading home the next morning. The jury has concluded that the prairie is just as pretty covered in a layer of snow, even if there’s not that much.
Forecasted snow turned up as rain in the Chicago area today. But this dreary morning was brightened by a delivery of crocheted Echinacea pens, courtesy of Hattie! Thank you for your craftsmanship!
Stuart presents Wyatt with a bouquet…
Old habits die hard. These are not for harvest, Wyatt!
You might think three weeks is too short a time to conduct research, but our 2023 Carleton College externs can prove you wrong! We just said goodbye to 4 undergraduate students who spent their winter break in our lab at Chicago Botanic Garden. During this time, they processed data, explored statistics, dove into the world of R, and learned more about the research objectives of the Echinacea Project. All of this contributed to their investigations into research questions of their own. The externs were also able to explore Chicago and the gardens, and connect with other scientists.
Rebecca and Vo worked with Jared to investigate effects of fire on reproduction in Andropogon gerardii. They developed methods for quantifying seed set in Andropogon, and put them into practice during the externship! Learn more about them and their research here and here!
Io worked with Abby and Wyatt to investigate vegetative and reproductive patterns in Echinacea Angustifolia. She was specifically interested in understanding how traits like basal leaf count and longest basal leaf length over time may influence reproductive effort. Read more about her project here.
Conlan worked with Abby and Wyatt to investigate structural reproductive traits and their relationship with pollination success. He wondered if taller head heights and larger head numbers led to higher pollination rates. Read more about what he found here!
There was some disagreement as to whether we were saying, “Echinacea” or “Andropogon” in our group picture this year. No matter- the photo still turned out nicely!
It was a pleasure to work with these 4 externs this year! We wish them the best!
I’m Rebecca Lerdau, a Junior at Carleton College. I’ve had a great time these past three weeks as a Carleton College extern at the Echinacea Project. I, and my classmate, Vo Dominguez were working with Jared Beck on the RemAg project looking at Andropogon gerardii’s (big bluestem) reproductive response to fire.
Having a good time counting the X-Ray images
A big part of our work has been with creating new protocols for determining Andropogon seed set. Previously, the main method for finding Andropogon seed set has been dissecting every floret, but this isn’t realistic on a large scale. Around half of Andropogon’s florets aren’t able to produce seeds, but it can be difficult to distinguish between the two types of florets. We wanted to figure out a way to use inflorescence mass to find total amounts of florets that can produce seeds. We were able to count awns and look at the relationship between awn count and mass. We found a beautifully linear relationship between awn count and seed mass (our R^2 = 0.96!) which allowed us to make an equation to use mass to determine the amount of fruiting florets. We set out to try X-raying Andropogon to determine seed counts. This was also successful, and we created a classification system for counting seeds with X-ray images.
We also got to try out our new seed set quantification system on some samples from the pilot RemAg experiment. We looked at the effects of burning on Andropogon seed set in 2022 from the pilot plots. While we were unable to find significant results with the data we were looking at, it was good to see that our protocols were working. The protocols are promising, and I look forward to seeing what happens with the larger RemAg project!
All in all, it’s been an awn-some experience working as an extern these past few weeks. I’ve learned so much. I’ve enjoyed getting to meet scientists and exploring CBG. We went on some lovely walks and got to see all sorts of cool plants. I had fun working with Andropogon and we even found a few seed predators! Thank you to Stuart, Jared, Wyatt, and Abby for this experience, and thank you to my fellow externs as well.
I am an aspiring botanist. I am specifically interested in ethnobotany/economic botany.
Statement
I am from Newton Grove, NC. In my spare time I like to read, listen to sad music, cook, and try/make new hot sauces
Projects
During my Fall ’23 externship, I processed wmSP and wmS0 heads to be used for Wyatt Mosiman‘s thesis. I used the resulting reproductive data from said heads with the corresponding plant measure data to assess the relationship between cumulative vegetative effort over 5 years (2018-2022) and reproductive effort and success in 2023. The path to my materials is: ~/Dropbox/ccExterns2023/ioHarris
I want to study biology and learn more about plant science.
Statement
I am from Houston, Texas.
In my spare time I like to play the trumpet and play games.
Echinacea Research Project
Introduction
The goal of my externship was a research project about Echinacea where I asked two questions and then conducted a study. The first question is does having taller head height increase pollination rates by insects in Echinacea. My second question is does having more heads increase or decrease pollination by insects in Echinacea.
Background
Echinacea is insect-pollinated mainly by bees and other studies I found have shown that in plants like C. behrii and V. thapsus taller heads are more likely to be pollinated because they are more visible to pollinators. Since we don’t know if head height plays a similar role in Echinacea I am conducting this study to see if there is any correlation between head height and pollination rates in Echinacea.
Another study I found saw more heads leading to greater pollination rates. However, Echinacea is self-incompatible, meaning it can not pollinate itself, unlike the plants in that study so its possible that if an insect stays at a single Echinacea plant with more heads instead of moving on to other plants it could actually hurt its pollination rate. This led me to my second research question to determine if there is a correlation between number of heads and pollination rates in echinacea.
Methods
I used data from a sample of about 229 heads that were put through a data collection process aimed at separated and analyzing the achenes in Echinacea heads. Achenes are the fruit that contain seeds in Echinacea heads so by analyzing if they contain seeds or not we can gather pollination data for this experiment. In the first step of the process we cleaned the heads for achenes. Then we rechecked our first count for accuracy and scanned the achenes into the computer for counting. Lastly, we randomized our achenes, separated them to be x-rayed, and classified them to see which achenes contained seeds giving us our pollination rates.
Question 1
After the data collection process the data was compiled into spreadsheets that were uploaded into R. I put the data on a scatterplot with head height vs pollination rate to create a regression line that had a positive correlation but was also very scattered. To see if that correlation was significant I conducted a linear regression analysis and found that it was significant so taller head height does seem to be related to higher pollination rates.
Question 2
I used boxplots to visualize this data with number of heads for an individual Echinacea plant vs average pollination rate and the pollination rate seems to go up as number of heads increase, the opposite of what I was expecting with self-incompatibility. I then conducted an ANOVA test and found that the difference in average pollination rate by number of heads was not significant so number of heads does not seem to have an effect on pollination rates.
Conclusion
My results gave a positive correlation between head height and pollination rate and no correlation between number of heads and average pollination rate. However, a big limitation to my study was the sample size, especially for question two. Only having one data point for the 5 and 7 heads categories could have a large effect on the data. Lastly, since this was an observational study we can only infer correlation not causation so someone conducting an actual experiment would be a good direction to go in the future.
Acknowledgements and Bibliography
Bibliography-
Dickson, C.R., Petit, S. Effect of individual height and labellum colour on the pollination of Caladenia (syn. Arachnorchis) behrii (Orchidaceae) in the northern Adelaide region, South Australia. Plant Syst. Evol. 262, 65–74 (2006). https://doi.org/10.1007/s00606-006-0472-3
LORTIE, Christopher J., and Lonnie W. AARSSEN. “The Advantage of Being Tall: Higher Flowers Receive More Pollen in Verbascum Thapsus L. (Scrophulariaceae).” Écoscience 6, no. 1 (1999): 68–71. http://www.jstor.org/stable/42901102.
Ohashi K, Yahara T. Effects of variation in flower number on pollinator visits in Cirsium purpuratum (Asteraceae). Am J Bot. 1998 Feb;85(2):219. PMID: 21684905.
Wagenius S, Lyon SP. Reproduction of Echinacea angustjfolia in fragmented prairie is pollen-limited but not pollinator-limited. Ecology. 2010 Mar;91(3):733-42. doi: 10.1890/08-1375.1. PMID: 20426332.
Thanks to Wyatt and Abby for helping me with data collection, Stuart for helping me develop ABTs and to the National Science Foundation for providing funding.
Materials that were used for my project can be found in the filepath: ~/Dropbox/ccExterns2023/conlanRamirez
For many, the end of the year brings thoughts of final exams, final reports, and final projects. Here at the Echinacea Project, we don’t believe in final anything, but we do believe in updates! Last week, two of our Lake Forest College interns, Sophia and Olivia, shared project updates with the lab: a culmination of their semester in the lab.
Olivia shared results from her study on plant and reproductive health indicators (more here)
Olivia and Sophia present research updates at lab meeting!
Sophia shared a poster with the lab and also with attendees of a Lake Forest College research symposium! Sophia’s research focuses on the effects of pollen limitation on life history fitness in Echinacea. (More info to come!)
Sophia Presents a poster at Lake Forest College’s Glassman Symposium.
