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Follow the tire tracks from the parking space at EELR north towards the crest of a hill and you will find several piles of yard waste that appear to have been dumped recently. I came across these piles on October 11th while pulling flags from summer experiments. Among the debris are many flowering stems of Echinacea purpurea.    Hailey hand pollinated flowers on six silver-leaf scurf pea plants (Pediomelum argophyllum). We are harvesting the plants as the pods ripen. Wyatt harvested two plants on 30 Aug, I harvested two today (9 Sept). Two remain–each is attached to a pin flag so it doesn’t tumble way. But don’t seem like that will happen soon. Yesterday I squeezed every pod from the plant that Wyatt harvested S of 23518-L. They all felt the same (empty), including the treatment pods that have yellow & blue twist-ties. Many of the pods had fallen off the stem, but not the bagged one. This time I gently put the entire plant into a large paper grocery bag to keep the pods on the stems. I regret i didn’t take any photos of the plants. Stay tuned to learn about effects of hand pollination on seed set in the silver leaf scurf pea! Read more about Hailey’s experiment. In past years team echinacea has noticed that silverleaf scurfpea very rarely produces a seed. Some would estimate over ninety percent of the time the pod contains no seed. This brings about many questions concerning why sliverleaf scurfpea produces so few seeds. The focus of this experiment is to get an idea of whether or not the problems related to going to seed are pollination related? The first step of the experiment was to was to look at the sliverleaf scurfpea’s flowers under a microscope to see if the flowers were producing pollen and see if it was possible to cross pollinate such a small flower in the field. The flowers of the sliverleaf scurfpea were producing pollen and with some experimentation I found a way to effectively cross pollinate the flowers. I found that the best way to get the pollen out out of the flowers was to use a dark colored toothpick and gently open the petals of the flower so that the anthers are more visible. following that I would lightly brush the anthers with the toothpick so that it would collect the pollen. I would then gently open the petals of the other flower and bush the toothpick over the stigma. The second phase of the experiment included going out into the field and cross pollinating the silverleaf scurfpea. This took place at the site Nice Island on July 24th and July 31st of 2024. The plants were chosen at random and each plant was over 20 meters away from the plant it was being crossed with. On each plant two flowers were randomly chosen. One as the flower that would be crossed, identified by a blue twist tie, and the other as a control variable, identified by a yellow twist tie. Following the cross pollination both twist tied flowers were covered in a pollinator exclusion bag. This was repeated for 6 different silverleaf scurfpea plants at Nice Island. Each plant involved in the silverleaf scurfpea experiment was marked using a green flag. This work sets the team up for the final step of the experiment. The final step will be to return to the cross pollinated plants in about 2-3 weeks and see if the pods that were pollinated produced a seed. This can be done by squeezing the pods. The pod will either contain a seed or not contain a seed.     Friday, 26 July, 2024, was the first day we started measuring p1 this year! Coincidentally, this was the same day we started last year. This monumental task involves visiting 10992 positions in the plot and assessing the status of the plants there (or not, in the case of plants we have not been able to find in >3 years. RIP. Presumably.) After measuring again this morning, I booted up the old progress tracker (in the cgData repo, if you ever need it) to see how we’re doing. After making some adjustments (for the colorblind out there), here’s our progress over the last two days:   Today was also RET Brittany’s last day with the field team. Brittany, you will be sorely missed, and we hope you bring a bit of the prairie to share with your students!  Big project update! For my summer project I am looking at nectar and pollen volumes in other Asteraceae plants that are non – Echinacea in burned sites versus unburned sites. This is important because pollinators depend on pollen and nectar to get the nutrients they need. A large decrease in pollinators has been found due to prairie fragmentation, and studying whether fire can help in producing a higher quantity of pollen and nectar can be shared with land managers to help create more viable resources for pollinators in their prairie restoration efforts. My two species of focus for now are Coreopsis palmata (also known as prairie coreopsis) and Helianthus pauciflorus (also known as stiff sunflower). Mid-summer coreopsis blooming is starting to come to close and so are my nectar and pollen measurements! Onto sunflowers here in the next couple weeks! Time for daily update: Total demo continues! One group of 4 and one group of 3 went out to a total of 4 different sites. These included south of golf course, north of golf course, and yellow orchid hill east and west. A few people also finished up or pollen and nectar collection on Echinacea heads. This afternoon a group went out and did e-trap collection and retrieval. We have been working on rotation 5 this week after finally polishing off rotation 4 late last week! The rest of us went and did measurements of Echinacea in one of our experimental plots (P9). Measurements included: flowering rosette count, basal rosette count, total basal leaves, height of tallest basal leaf, height of tallest cauline leaf (aka leaf on stem without a petiole), and height of head to name a few. Our plant of the day was finding a diseased Echinacea with over 360 basal leaves. To finish off the day we moved the goats to a new paddock and enjoyed some cold watermelon in the late week heat.   Total demo has commenced! Team Echinacea began to assess demography of flowering and non flowering Echinacea today! We started at tower, a site with a rich demographic history. We payed special attention to tag 9301- a plant first tagged in 1996. Many members of Team Echinacea have met this plant before. Including me last year. This year, 9301 is basal and has 4 rosettes. I hope it has many more fruitful summers in store. We’ve got 2,640 locations where we will search for Echinacea in 2024. Our work is cut out for us, but after just one morning in the field, we are already 3.75 % done!
 Today was the first day of total demography! Team Echinacea started as a whole group at Tower, a remnant prairie site, where Jared gave an overview of total demography. Team Echinacea learned important skills such as communication and collaboration. After total demography was finished at Tower, one group headed to Martinson Approach and another went to East of Town Hall. Echinacea angustifolia pollen has seemed to wind down in the past few days. Today, Grace and Rebecca collected the remaining pollen from the last flowering plants. In the afternoon, everyone had personal project time! Today Team Echinacea continued to wrap up the pollen and nectar collection. Only a few focal plants are still in flower. Another group worked on flagging and recording demographic information for every flowering Echinacea plant in every remnant site. Some of these plants have tags dating back decades! In the experimental plots, Stuart trained team members to find and measure all Echinacea. This data will help us understand performance of E. angustifolia x pallida hybrids. Round 5 of emergence trapping started recently. The team members are now pros at deploying and retrieving the traps.  It’s heating up in western Minnesota! Temperatures and humidity were high today. In the morning, much of Team Echinacea performed the pollinator observations we learned about yesterday with Dr. Ison. These involved recording the pollinator with a camcorder, describing its activities, and attempting to ID to morphospecies level.  In the afternoon, emergence trapping crews had a great session of deployments and retrievals, while others worked on phenology in the common garden plots, and others gathered nectar. It’s exciting to see new species starting to bloom: two major species of prairie clover are just getting started, as is the wild bergamont and wild licorice.  Once everyone was back from the field, we enjoyed some ice cold watermelon and headed home. Another great day in the field! This week was a busy Monday for team Echinacea. Jennifer from College of Wooster joins us this week along with her research assistants Max and Indigo! This morning started off with round 2 of sweet clover pulling, and was promptly followed by a crash course in pollinator observations. We tried our hand in collecting species in small tubes for ID and then release. A big part of this was learning how to differentiate between flys and bees as some flies can look realllyyy close to a bee. But don’t be fooled! To round out the morning we went out in groups to collect pollen for our pollen and nectar study specifically looking at echinacea angustifolia. After lunch, half of us went and deployed another round of emergence traps while the other half went out and did nectar collection for the pollen and nectar study.  Maddie with round 2 of our sweet clover pulling bounty.  Jennifer prepping us for our crash course in pollinator observations.  Zach holding a micro-capillary tube with a whopping 7mm of nectar! |
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