Total demo has commenced! Team Echinacea began to assess demography of flowering and non flowering Echinacea today!
We started at tower, a site with a rich demographic history. We payed special attention to tag 9301- a plant first tagged in 1996. Many members of Team Echinacea have met this plant before. Including me last year. This year, 9301 is basal and has 4 rosettes. I hope it has many more fruitful summers in store.
We’ve got 2,640 locations where we will search for Echinacea in 2024. Our work is cut out for us, but after just one morning in the field, we are already 3.75 % done!
Today Team Echinacea continued to wrap up the pollen and nectar collection. Only a few focal plants are still in flower. Another group worked on flagging and recording demographic information for every flowering Echinacea plant in every remnant site. Some of these plants have tags dating back decades! In the experimental plots, Stuart trained team members to find and measure all Echinacea. This data will help us understand performance of E. angustifolia x pallida hybrids. Round 5 of emergence trapping started recently. The team members are now pros at deploying and retrieving the traps.
It’s heating up in western Minnesota! Temperatures and humidity were high today. In the morning, much of Team Echinacea performed the pollinator observations we learned about yesterday with Dr. Ison. These involved recording the pollinator with a camcorder, describing its activities, and attempting to ID to morphospecies level.
Lots of echinacea in bloom at Torgeson north.
In the afternoon, emergence trapping crews had a great session of deployments and retrievals, while others worked on phenology in the common garden plots, and others gathered nectar. It’s exciting to see new species starting to bloom: two major species of prairie clover are just getting started, as is the wild bergamont and wild licorice.
Daleapurpurea, or purple prairie clover, in bloom at Hulzbos CRP north.
Once everyone was back from the field, we enjoyed some ice cold watermelon and headed home. Another great day in the field!
This morning the whole team got together to pull as much sweet clover as we could from p01. After the drippy morning faded, we split up to collect pollen from Echinacea angustifolia at sites throughout the area. This is part of an MN ENRTF funded project to help determine the impacts of prescribed burns on pollen and nectar quantity and quality in echinacea. After lunch, some people went to retrieve and deploy emergence traps and the rest of us went to collect nectar from echinacea plants. To collect the nectar, we are using tiny glass microcap tubes carefully inserted into the florets. Overall, we had a productive day, and enjoyed the nice weather after a wet 4th!
The beginning of flowering for Echinacea angustifolia means it’s time for Team Echinacea to get collect Echinacea pollen and nectar! This project is part of our MN ENRTF funded research to understand fire’s impacts on ground nesting bee habitat and food resources. It’s our second year of sampling and we have made some modifications and improvements to our protocol.
This year we are collecting from a total of 62 plants across 12 sites (some burned in 2023, some in 2024, and a few not burned in either year). Where possible, we are collecting from plants that we also collected from last year, which will make for some interesting comparisons. Many plants did not re-flower in 2024, so Wyatt and I bolstered our sample by randomly selecting other focal plants.
On Monday, Wyatt and I visited our first flowering focal plants with Grace and Rebecca from NDSU and tested out methods. We learned how to sample immature florets, a new method we are using to assess pollen quantity this year. We also brushed up on nectar and pollen collection.
Wyatt collects pollen from the anthers of a flowering Echinacea
By Tuesday we were able to train the whole team in on the protocol and start collecting! So far our data sheets have worked pretty well, and we are figuring out ways to improve efficiency in the field. Stay tuned as more of our focal plants begin to flower!
Today we welcomed goats to Hjelm. They are already hard at work eating their way through the foliage. Keep up the good work, goats! Most of the humans worked on finishing searching for Stipa in p01 and started planting a new production garden to generate seed to add to the experimental plots. Ian and Liam valiantly continued staking points for the pollinator emergence study. Wyatt and Abby are getting ready to begin a study investigating the effects of fire on pollen and nectar production in Echinacea angustifolia. This project has many excellent collaborators and is part of the MN ENTRF funded research on prescribed fire and ground nesting bees.
A smooth start to the first emergence trapping deployment! Today we placed 23 traps at three sites, making good use of our new color-coded flagging system. So cool to see our work from the last few weeks starting to pay off!
Some emergence trapping sites are a lot smaller than others! It will be interesting to see whether size is associated with catch rate. Do bees prefer to nest in big sites? REU student Zach deploys a trap at our first site of the day. This site was burned earlier in the spring, can you see how short the vegetation is?
These traps will be out for four days, then emptied and sent back out to new sites on Monday. Fingers crossed we’ll see some bees!
NOTE: Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR).
We have a patch of an invasive weed in our main experimental plot. We have been trying to keep this non-native hawkweed from spreading for several years. This year we did a really good job. First, we found all the satellite patches and used the opportunity to practice reading and making maps. Then we pulled all the flowers stems–we estimated 369 stems. Here’s a map of all hawkweed patches and the main infestations is the 10m x 10m square.
Then we pulled all the stems in the square of infestation, counting as we pulled. Here are the counts & the total in the square:
After we counted and pulled, we each made an independent estimate of the number of flowering stems we missed. We could have missed stems that were trampled or difficult to see with the current light conditions. Here are our counts with the mean and median:
Stuart went back when it was cloudy and found 47 stems in the infestation square, mostly trampled. The next day he systematically walked the square and found 4 more. So that’s 51 missed. One person had a pretty close estimate (48). All but one of our estimates were optimistic about our thoroughness.
round(c(37,51)/(1265+51), 3) [1] 0.028 0.039
We estimated a miss rate of 3% and our actual rate was closer to 4%. We practiced estimating, pulled a lot of stems, ~1700, and have maps to go back and get the plants later this season. Good work, team!
As part of my recent independent study with Stuart and Northwestern undergrad Lena Parnassa this spring, I took an initial pass at analyzing the data from our 2023 emergence traps. These data are an important contribution to the broader ENRTF project, which is geared at understanding how ground-nesting bees respond to prescribed fire in prairies. This independent study enabled me to learn a great deal about database organization, coding, and collaboration between data scientists. Before we could run any of our analyses, we had to wade through quite a bit of data cleaning to ensure all our joins were functioning properly. This meant that we had to prioritize the bee analysis rather than the bycatch in our traps (millipedes, grasshoppers, and so forth), so stay tuned for more in that department! We were also working with abundance data rather than species data, as identifications of all the bees we caught in 2023 are still forthcoming. This means that some of the data points in our analysis are most likely incidental, but nevertheless, here’s a visual of what we found!
Figure 1: Mean bee catch rate by site type. Catch rate refers to the proportion of traps which had bees.
Figure 2: Bee catch rate by site. Catch rate refers to the proportion of traps which had bees.
Mean bee catch rate by burn history. Only three sites were 3 years post-burn in 2023, other burn treatments had 7-8 sites.
It looks like remnant prairies had more bees than restorations, but there’s a ton of variation across sites of the same land use type. It’ll be interesting to see what other factors may be associated with this variation, if any. Once we get those species ID’s and confer with our taxonomist, we’d like to tweak our protocol slightly going into this summer to maximize our chances of catching the bees at a given site. We’re considering stocking our traps with propylene glycol to better preserve specimens, trimming the vegetation around traps to make sure bees can find the entrance, and deploying later in the day to give foraging bees time to find their way back to their nests. Looking forward to kicking of the 2024 trapping season!
NOTE: Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR).
NU Work Study student, Maria, took on the task of xraying Andropongon samples for our project investigating fire’s impact on reproduction in big bluestem (Andropogon gerardii). We were about two samples in when we noticed that either this “lil box” containing samples was either very big or Maria had gotten very small.
Normal Boxes you’d find around our labMaria with giant box unlike things we commonly see
