We’re interested in investigating what resources are available to Echinacea visitors and learning more about the pollen and nectar Echinacea produces. We hope to learn if the nutritional resources available differ before and after burns. In 2022, Britney House developed methods for collecting nectar from Echinacea using microcapillary tubes. Read more about her methods here.
During the summer of 2023, the team collected pollen and nectar samples from Echinacea angustifolia at 19 sites in and around Solem Township, MN (plants at Hegg Lake were Echinacea pallida). We searched for and shot the ~20 plants (or, if few were available, as many as we could find) at each site that were closest to a random point. We then selected ten of those plants to bag up to three of their heads with pollinator exclusion bags. Throughout the duration of their flowering, we collected pollen from all bagged plants and nectar from five of them per site.
Stuart collects nectar from an Echinacea head
Midway through the experiment (mid-July), we removed bags from some of the pollen only plants to adjust our sample size to only the five pollen/nectar plants plus two backups per site. We removed bags from pollen/nectar plants and backup plants when they were done flowering, we’d collected a cumulative 50 mm of nectar from them, or we had received less than 15 mm of nectar from the plant in the last three visits (the latter was more of a guideline than a rule, used to save time by eliminating plants that were unlikely to provide us with enough nectar for analysis). At the Hegg pallida restoration, any heads that were not originally bagged were decapitated, and all heads were decapitated upon the final removal of their bags.
Following some experimentation, we conducted nectar collection only in the afternoons, while pollen collection could be done any time of day. In total, we collected 856 vials of pollen and 580 vials of nectar from 104 plants. These were given to Rahul Roy and Margaret Medini at St. Catherine University in St. Paul, who will be doing data analysis.
Data entry for collection datasheets is complete, and verified csvs for each site can be found at: Dropbox/teamEchinacea2023/z.pollenNectarDataEntry/coreVerified. Scans can be found at: Dropbox/teamEchinacea2023/z.pollenNectarDataEntry/scans.
Start year: 2023
Location: Various prairie remnants and one restoration (Hegg Pallida) around Solem Township, MN
During the summer of 2023, Team Echinacea conducted floral surveys at randomly selected bb points in remnant prairies and restorations. We are interested in quantifying floral resources (i.e., food for bees) and we want to understand how fire influences the diversity and abundance of flowering plants.
At each focal point (bbpt) we identified species rooted within a 2 meter radius and recorded the furthest stage of development. We measured abundance by binning a range of floral units (i.e., 1-5 flowering units got label “5”).
Floral surveys were split into “visit group A” and “visit group B”. We surveyed different random points when revisiting sites. In total, we conducted 415 floral surveys across 45 sites.
Liam Poitra, a 2023 Summer Research Experience for Undergraduates (REU) Participant, contributed to this research project investigating the effects of fire on diversity and abundance of flowering plants. Liam assisted in fieldwork and data organization. Inventory, protocols, and blank datasheets for floral assessments are located in ~/Dropbox/enrtf/floralSurveys2023.
Liam Poitra, REU 2023, navigates to a floral assessment focal point at Staffanson Prairie Preserve. The 2-meter stick he carries will help keep track of what is in the bounds for survey.
Start year: 2023
Location: prairie remnants and restorations in Solem Township
In July, 2023, a team from Minnesota State University, Mankato visited Team Echinacea to sample prairie soil. They are investigating how fire and management history influences physical, chemical and biological soil properties. Differences in soils may also help explain plant fitness and native bee nesting patterns. After three hot days, 263 samples were collected from 28 sites!
Back in Mankato, four undergraduate students processed the soil samples by sieving and weighing out the field collections. The preliminary results show soil bulk density is lower in remnant prairies compared to restored prairies. This makes sense in that remnant prairies have retained their organic-matter rich topsoil, infiltrated with plant roots and filled with pore spaces that develop over time in natural prairies. The soil bulk density is also lower on flat (no slope) topography, in part due to illuviation (deposition) of nutrients and organic material from nearby sloped areas.
The team hopes to continue its investigations to understand which soil properties are sensitive to different management histories and how the patchwork of fragmented prairies in the region vary in carbon, nitrogen and biological activity. These data should provide foundational information for many additional projects.
The Mankato soil team gathers around a bbPoint to discuss sampling. The team visited bbPoints in remnants and restorations over a 3 day period.
Start year: 2023
Location: : 28 study plots on private, federal and state land with different land use histories: remnant and restored grassland
Data collected: Soil bulk density, maximum water-holding capacity, gravimetric soil moisture content, soil aggregate stability (SLAKES), total organic C, total N, inorganic N (plant available forms: ammonium and nitrate), aerobic respiration rate (proxy for microbial activity and decomposition)
Samples or specimens collected: Topsoil bulk density and additional soil (top 15 cm) for each bee nesting trap
Many plants, including Echinacea angustifolia, flower vigorously during the summer after a prescribed burn. We’ve demonstrated that the benefits of fire for seed production, in many circumstances, are bigger than just the increase in flowering. The additional boost to seed production results from better pollination after fires compared to other times. Now we are trying to figure out what’s going on with pollination–why is it better after a fire? It might be related to pollen or nectar, which are foods for the bees that pollinate Echinacea. Here are two possibilities: 1) after a fire, plants produce more or better pollen or nectar which draws in bees from farther away, so the plants get more visits and better pollination, presumably the bees are happier with abundant & healthy food. 2) after a fire, plants produce less or lower quality pollen or nectar which means bees need to fly to more plants to get a decent meal, so the plants get more visits, and the bees are probably frustrated with skimpier meals and bad food. The third possibility is that plants produce the same quality and quantity of pollen & nectar regardless of fires.
Over the summer we systematically collected pollen and nectar from many Echinacea plants in many populations (19) over many days. Our goal is to evaluate how fires affects the quality and quantity of pollen & nectar produced by Echinacea plants. We are getting close to wrapping up data-entry for our field collection of pollen and nectar from Echinacea angustifolia. Here’s a summary of data-entry progress so far…
Each “tagCt” is the number of Echinacea plants we sampled at each site. We will keep you posted!
Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR).
Today, while deploying emergence traps, we avenged our recently stung colleague (Ellysa Johnson). One of our randomly selected points was directly upon a hornets nest (a few are seen in this photo, but tens were present and buzzing furiously). Miraculously, neither of the crew members present were stung. Let this day mark our revenge.
Today was Harrison’s last day with us 🙁 Stuart made a prairie-inspired cake to honor his time with the project. He is returning to teach young minds about ecological research. Farewell, Harrison, and good luck!
The team also conducted floral abundance surveys. Essentially, we want to see how many plants (and what kinds) may be associated with ground-nesting bees. That requires feet on the ground to estimate abundances and identify plants.
Lastly, a battle occurred today. While the crew did demography of echinacea plants- where we record data on this year’s flowering plants- I was stung by two wasps. Luckily, Lindsey was prepared to retaliate, though it wasn’t necessary.
All in all, farewells can sting, but at least there’s cake in the end.
Grass so high Our vision fails Of flags off hiding Who knows where
Holes for hiding Insects who Like their grasses Tall and true
Today we helped dig soil cores for a “pitfall trap” project. The holes were dug near our ENRTF insect collection points and will grant greater insight on how burning prairies can affect insect population composition. The grass was pretty high, though, and we could hardly see the flags that had been placed earlier in the season! Rest assured, the holes were dug.
Also we did emergence traps. We always do emergence traps.
The pollinator team set out to recover some emergence traps (picture 1) this afternoon. While we didn’t find ground-nesting bees, which this project is centered around, we did see another pollinator while sifting through grass that extended beyond our own heads (Jan for scale; picture 2). The viceroy (Limenitis archippus; picture 3) looks incredibly similar to the monarch (Danaus plexippus), except for the black, horizontal line that cuts across their dorsal wings. We hope to see even more pollinator friends as the field season goes on!
To counter expected high temperatures, we started earlier in the morning with GPS points for the ENTRF-funded bee research project and found some cool plants. After lunch, some of the team continued to stake and shoot points, while others planted some green comet milkweed (Asclepias viridiflora) seedlings in a previously burned site. We also saw a baby Pheobe on the deck after it attempted to fledge.
One of our hard-working employees finding points with our handy dandy GPS units.Pheobe fledgling (or attemptee).Alumroot (Heuchera richardsonii) at one of our sites.
