A close up of an Agapostemon specimen with its tongue sticking out. The current collection of bees. Many more to come!
As of a few weeks ago, Emergence trapping for the ENRTF project is finished! It was a massive endeavor to complete, and I’m thankful for everyone on the team who pitched in. Now that our vials of bugs have been moved from Minnesota to Illinois, it’s time to start going through them to look for bees. It’s smelly work, but also a great opportunity to brush up on taxonomy and identification skills. We’re planning to hire a work-study student to help with pinning and data entry in the next few days. Until then, more for me!
NOTE: Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR).
Currently, I’m writing this from my new apartment in Chicago! Classes start next week for me, and I’m moving on to the data management portion of my summer project. This summer, I looked at pollen and nectar quantity in Coreopsis palmata (prairie coreopsis) in burned vs. unburned sites. But why does this matter? Pollinators depend on pollen and nectar from flowers to supply the nutrients they need to survive. However, there has yet to be a strong comparison between common Asteraceae plants identifying the quantity and quality of plants that can be shared with land managers to help create more viable resources for pollinators in their prairie restoration efforts. Therefore, I wanted to conduct a study looking at pollen and nectar quantity in common non-Echinacea prairie plants to examine how prescribed fire affects plant resources for pollinators.
The sites I collected data from included Torgeson North and South, Tower and Nice Island, and Yellow Orchid Hill West. Pollen data was collected by selecting three immature florets from three flower heads in a “patch,” defined as five or more stems in one central location. These immature florets will then go through a lab process where they are sliced open and placed into an agar solution, allowing the pollen to float to the top, where it can be counted. Nectar collection was done using microcapillary tubes, where we measured the amount of nectar in each tube in millimeters. The volume of nectar can then be calculated based on the size of the microcapillary tube in relation to the measurement in millimeters obtained from the tube. In total, I had around 75 pollen and nectar measurements. I also collected transect density data for each “patch” that we sampled and mapped them in Avenza for future reference. This allows me to identify and see how dense the populations are within a certain site, and to determine if there is a difference between burned vs. unburned areas.
I was not able to study more than one species this summer, but we shall see what the next summer holds!
NOTE: Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR).
With a smaller crew and slightly soggy start to the morning, it was all-hands-on-deck as we continue to chip away at measuring experimental plot 1. We made great progress today, but still found time to stop and take pictures of cool critters! After lunch, we all headed out to retrieve and deploy emergence traps. It was a busy day for e-trapping, with three teams visiting eight sites in total.
Big beautiful eyes, such a shiny green, gorgeous!Absolute stunner. (bit Wyatt, but we forgive her)
This week was a busy Monday for team Echinacea. Jennifer from College of Wooster joins us this week along with her research assistants Max and Indigo! This morning started off with round 2 of sweet clover pulling, and was promptly followed by a crash course in pollinator observations. We tried our hand in collecting species in small tubes for ID and then release. A big part of this was learning how to differentiate between flys and bees as some flies can look realllyyy close to a bee. But don’t be fooled! To round out the morning we went out in groups to collect pollen for our pollen and nectar study specifically looking at echinacea angustifolia.
After lunch, half of us went and deployed another round of emergence traps while the other half went out and did nectar collection for the pollen and nectar study.
Maddie with round 2 of our sweet clover pulling bounty.
Jennifer prepping us for our crash course in pollinator observations.
Zach holding a micro-capillary tube with a whopping 7mm of nectar!
This morning the whole team got together to pull as much sweet clover as we could from p01. After the drippy morning faded, we split up to collect pollen from Echinacea angustifolia at sites throughout the area. This is part of an MN ENRTF funded project to help determine the impacts of prescribed burns on pollen and nectar quantity and quality in echinacea. After lunch, some people went to retrieve and deploy emergence traps and the rest of us went to collect nectar from echinacea plants. To collect the nectar, we are using tiny glass microcap tubes carefully inserted into the florets. Overall, we had a productive day, and enjoyed the nice weather after a wet 4th!
The beginning of flowering for Echinacea angustifolia means it’s time for Team Echinacea to get collect Echinacea pollen and nectar! This project is part of our MN ENRTF funded research to understand fire’s impacts on ground nesting bee habitat and food resources. It’s our second year of sampling and we have made some modifications and improvements to our protocol.
This year we are collecting from a total of 62 plants across 12 sites (some burned in 2023, some in 2024, and a few not burned in either year). Where possible, we are collecting from plants that we also collected from last year, which will make for some interesting comparisons. Many plants did not re-flower in 2024, so Wyatt and I bolstered our sample by randomly selecting other focal plants.
On Monday, Wyatt and I visited our first flowering focal plants with Grace and Rebecca from NDSU and tested out methods. We learned how to sample immature florets, a new method we are using to assess pollen quantity this year. We also brushed up on nectar and pollen collection.
Wyatt collects pollen from the anthers of a flowering Echinacea
By Tuesday we were able to train the whole team in on the protocol and start collecting! So far our data sheets have worked pretty well, and we are figuring out ways to improve efficiency in the field. Stay tuned as more of our focal plants begin to flower!
A smooth start to the first emergence trapping deployment! Today we placed 23 traps at three sites, making good use of our new color-coded flagging system. So cool to see our work from the last few weeks starting to pay off!
Some emergence trapping sites are a lot smaller than others! It will be interesting to see whether size is associated with catch rate. Do bees prefer to nest in big sites? REU student Zach deploys a trap at our first site of the day. This site was burned earlier in the spring, can you see how short the vegetation is?
These traps will be out for four days, then emptied and sent back out to new sites on Monday. Fingers crossed we’ll see some bees!
NOTE: Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR).
As part of my recent independent study with Stuart and Northwestern undergrad Lena Parnassa this spring, I took an initial pass at analyzing the data from our 2023 emergence traps. These data are an important contribution to the broader ENRTF project, which is geared at understanding how ground-nesting bees respond to prescribed fire in prairies. This independent study enabled me to learn a great deal about database organization, coding, and collaboration between data scientists. Before we could run any of our analyses, we had to wade through quite a bit of data cleaning to ensure all our joins were functioning properly. This meant that we had to prioritize the bee analysis rather than the bycatch in our traps (millipedes, grasshoppers, and so forth), so stay tuned for more in that department! We were also working with abundance data rather than species data, as identifications of all the bees we caught in 2023 are still forthcoming. This means that some of the data points in our analysis are most likely incidental, but nevertheless, here’s a visual of what we found!
Figure 1: Mean bee catch rate by site type. Catch rate refers to the proportion of traps which had bees.
Figure 2: Bee catch rate by site. Catch rate refers to the proportion of traps which had bees.
Mean bee catch rate by burn history. Only three sites were 3 years post-burn in 2023, other burn treatments had 7-8 sites.
It looks like remnant prairies had more bees than restorations, but there’s a ton of variation across sites of the same land use type. It’ll be interesting to see what other factors may be associated with this variation, if any. Once we get those species ID’s and confer with our taxonomist, we’d like to tweak our protocol slightly going into this summer to maximize our chances of catching the bees at a given site. We’re considering stocking our traps with propylene glycol to better preserve specimens, trimming the vegetation around traps to make sure bees can find the entrance, and deploying later in the day to give foraging bees time to find their way back to their nests. Looking forward to kicking of the 2024 trapping season!
NOTE: Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR).
Members of Team Echinacea are freshly returned from a successful burn outing! We completed five experimental burns during our trip to western MN. A major win for the experimental design of our MN ENRTF funded research on prescribed fire and ground nesting bees as well as for conservation! Here is the scoop:
Late Friday night we got to the Hjelm House and were lucky enough to catch a glimpse of the Aurora Borealis. In hindsight, I think this auspicious sighting boded well for our good fortune in burning conditions.
On Saturday morning we headed to Torgeson’s to burn the northern unit. Because it was predicted to be a dry day, we got an early start and were ready to burn by 10:45. We got to see lots of spring flora before the burn: pucoons, violets, sedges, pussytoes, etc… These plants will be even happier next year. With help from Lee, the burn went very smoothly. Torgeson’s is a hilly site, so the burn was slope driven and we had a great view. Things are pretty greened up in the area, so the burn was quite smoky. USFWS, who were burning a big restoration right next door must have been burning cattails- look at that thick, brown smoke coming from the other side of the road. Almost like we are having bonfires at neighboring campsites. Cute!
Jared secures the break at torgen before we stand back and watch the fire burn out.
Next we ventured into Grant County. No burning there- and if you’ve been following along that may not be a surprise to you. So it was back to Douglas Co. After lunch the crew headed to hutch to burn the western unit. Another Hilly site. We prepped our breaks and were ready to go! Burn number 2 down!
Sunday was a no-burn day because wildfire smoke from Canada was hanging over many counties in MN. A great day to get work done on the porch and venture into Morris to revisit old haunts. We anxiously awaited the next day’s updated report on burn restrictions.
Monday morning we got the go ahead to burn in Grant county!! We called in our backups (former team members Daytona and Liam plus a few of Liam’s friends) and headed to Yellow Orchid Hill West to train our first time burners in at a smaller site. The wind was squirrely but our burn was successful and we were just a hop, skip and a jump away from the revered hulze unit. After 3 failed attempts, was this finally our day?
Stuart and Jared watch the last flames burn out in the center of hulze.
Yes! Drive down highway 55 and you will see for yourself! A hilly expanse of charred earth! A sight for sore eyes after the last burn way, way back in 2003.
By this time, restrictions were lifted in Douglas Co. and the crew headed to Nice Island for a final burn before calling it a day and heading back to the farmhouse. Over Jean’s rhubarb cake we envisioned a utopian or maybe dystopian future on team Echinacea where we have a high tech call center and everything is drone operated. We’ll keep you posted on the call center and the rest of the burns we hope to get done this season.
There are so many interesting questions to ask within the study design of our MN ENRTF funded research on prescribed fire and ground nesting bees! We are lucky to collaborate with researchers in the area who are taking them on.
Justin presents his research at the MSU-Mankato research symposium
Justin Kjorness, undergraduate at Minnesota State University-Mankato, worked with Drs. Mrganka De and Matt Kaproth (and many more) to collect soil samples at our remnants and restorations this summer. He has since been asking questions about the effects of prescribed fire regimes on soil physical properties. He presented these results at the MSU-Mankato research symposium this week!
