Experimental plot one parasite planting update

Over the last two years I designed and planted an experiment in an already established prairie restoration (exPt01) to test hypotheses about the effects of parasite inclusion in restorations. This experimental planting of hemiparasites has three factors (Comandra umbellata, Pedicularis canadensis, and soil plugs), each with two levels (presence or absence), but three factor-level combinations are impossible because the presence of parasites is confounded with presence of soil. This translates to me having 216 row x position combinations in which I randomly assigned Comandra umbellata, Pedicularis canadensis, and soil plugs. However, roots trap soil and therefore soil is always carried in with parasites, the two treatments are confounded and so we used soil transplants to account for this.

In June, I went out and assessed the realized design of my experimental planting of hemiparasites. I assessed presence or absence of Comandra umbellata and Pedicularis canadensis at each of my 216 row x position combinations. I found only one Comandra but I found 30/72 or 42% of all Pedicularis.

In late August through early September, I planted 1 plug of Liatris ligulistylis and one plug of Solidago speciosa at each of my 216 locations.  These plants were then measured. I added these plugs to serve as response variables to my three-factored experiment.

Last year, at all 216 locations I distributed seeds from 32 native plant species. In September, I went out and assessed seedlings present. I recorded the number and photographed the seedling to identify later in the laboratory.

In late October I intend to harvest 216 strips (0.1m x 1.0m) of dried biomass (1/6th of the dried biomass) from my 216 locations as I have also done in the last 2 years.

Start year: 2019

Location: Douglas County, Minnesota; exPt 1

Overlaps with: Experimental plot management, Hesperostipa common garden experiment

Materials collected: 216 .1 x 1m strips of dried biomass are stored at the Chicago Botanic Garden.


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