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Liatrus Project Update #1

I am happy to say that the Liatrus Project is off to a good start. After taking inventory of all the Liatrus plants this past week, I got to start the cleaning process. A total of 293 Liatrus plants have been counted in the inventory, and all have been sorted randomly into 5 different batches. Today, I got to start cleaning the ones in the 1st batch, and while cleaning, I noticed several similarities and differences compared to cleaning Echinacea plants. Overall, I found that Liatrus achenes were much easier to extract from the plant than Echinacea achenes, but counting them proved much more challenging. To make things easier, random selection sheets of different numerical ranges were arranged that listed random numbers from left to right down the sheets. Using these sheets, I could randomly pick out a flower head and count the number of achenes associated with that head. I also had to observe if any achenes were missing from each head on a Liatrus plant. I recorded the total number of heads per plant and the number of heads with no achenes, some achenes, or all achenes missing. After taking these recordings, I removed all the other achenes present on the Liatrus plants and sorted them into an envelope. Any chaff leftover got put into a separate envelope labeled as “chaff.” So far, a handful of plants have been cleaned, but there is still a long way to go.

Beginning of “The Liatris Project”

Today will mark the beginning of a new project that I will conduct analyzing Liatris aspera (Rough Blazing Star). Like with the Echinacea Project, this project will look at reproductive quantities of Liatris and the potential factors for influencing plant reproduction. At the moment, a specific research question is still in the works and the actual project requires some introductory steps that need to be completed. In the lab, I conducted inventory checks for the Liatris plants that have been harvested and made sure their were not any errors in what was taken into inventory. While doing the checking, I had Leah help me make sure everything was accounted for. Trying to do this alone would have been frustrating so I send my absolute thanks for helping me out with this part. As for the next steps in the project, I hope to begin cleaning the Liatris plants next week and start to come up with a potential research question in the near future. Very exciting things to come!

CJ Myers

Echinacea Project 2023

Major: Environmental Studies

Institution: Lake Forest College 

Expected year of graduation: 2023

Research Interests

I’ve always had a keen interest in exploring and learning about the environment, and one of the biggest things I want to study and research are methods in which we can help our environment and promote a better world. 

Hobbies

I like to spend time with my family and watch sports in my spare time. I also like to play games and relax outdoors if the weather is nice.

Lab meeting: Disruptive science

Resources for lab meeting (1/13):

Discussion questions:

  1. What paradigm shifts have occurred in the fields of ecology and plant science? Have any occurred recently?
  2. Conversely, have you observed new research in the field becoming less disruptive over time? If so, do you agree with the authors’ suggestion that this can be attributed to researchers focusing on “narrower slices of previous work”? (142)
  3. What is your perception of the paper’s measure of disruptiveness (CD5)? What might be some strengths and weaknesses of this approach?
  4. Do the popular media pieces effectively communicate the paper’s findings to a general audience? What angles do they take in their explainers, and why?
  5. Though papers have become less disruptive on average, the quantity of disruptive papers has remained largely consistent. Do you agree with the paper’s proposal of a carrying capacity for highly disruptive papers?
  6. Is a decline in disruptiveness exclusively a bad thing? Consider Kuhn’s ideas on “paradigms.”

2022 Update: Dykstra’s local adaptation experiment

This experiment was designed to quantify how well Echinacea angustifolia populations are adapted to their local environments. In 2008, Amy Dykstra collected achenes from Echinacea populations in western South Dakota, central South Dakota, and Minnesota and then sowed seeds from all three sources into experimental plots near each collection site. Each year, Team Echinacea takes a demographic census at the western South Dakota and Minnesota plots; we abandoned the central South Dakota plot after it was inadvertently sprayed in 2009, killing all the Echinacea.

In 2022, during the annual census of the experimental plots, we found 135 living Echinacea plants, including 102 basal plants and 33 flowering plants. All but two of the flowering plants were in the South Dakota plot. A wetter-than-average spring may have contributed to the flowering output of these plants. The South Dakota prairies were as green as Amy can remember seeing.

Pictured is one of the flowering plants in the South Dakota plot. We performed the census on June 30, before the flowering heads started dehiscing pollen.
  • Start year: 2008
  • Location: Grand River National Grassland (Western South Dakota), Samuel H. Ordway Prairie (Central South Dakota), Staffanson Prairie Preserve (West Central Minnesota), and Hegg Lake WMA (West Central Minnesota).
  • Overlaps with: Dykstra’s interpopulation crosses
  • Data collected: Plant fitness measurements (plant status, number of rosettes, number of leaves, and length of longest leaf)
  • Samples collected: Heads from all flowering plants; Amy stores the heads in her office at Bethel University.
  • Products: Dykstra, A. B. 2013. Seedling recruitment in fragmented populations of Echinacea angustifolia. Ph.D. Dissertation. University of Minnesota. PDF

You can read more about Dykstra’s local adaptation experiment and see a map of the seed source sites on the background page for this experiment.

2022 Update: Dykstra’s interpopulation crosses

Small remnant Echinacea populations may suffer from inbreeding depression. To assess whether gene flow (in the form of pollen) from another population could “rescue” these populations from inbreeding depression, we hand-pollinated Echinacea from six different prairie remnants with pollen from a large prairie remnant (Staffanson Prairie) and from a relatively small population (Northwest Landfill) in 2008. We also performed within-population crosses as a control. Amy Dykstra (with help from Caroline Ridley) planted the achenes (seeds) that resulted from these crosses in an experimental plot at Hegg Lake WMA.

We sowed a total of 15,491 achenes in 2008. 449 of these achenes germinated and emerged as seedlings. Each summer, we census the surviving plants and measure them.

In 2022, we found 26 surviving plants; all were basal. Joey McGarry staked the plant locations, Amy Dykstra searched for the plants, and Brad Dykstra recorded the data.

  • Start year: 2008
  • Location: Hegg Lake WMA
  • Overlaps with: Dykstra’s local adaptation
  • Data collected: Plant fitness measurements (plant status, number of rosettes, number of leaves, and length of longest leaf), and notes about herbivory. Contact Amy Dykstra to access this data.
  • Samples collected: NA
  • Products: Dykstra, A. B. 2013. Seedling recruitment in fragmented populations of Echinacea angustifolia. Ph.D. Dissertation. University of Minnesota. PDF

You can read more about Dykstra’s interpopulation crosses, as well as links to prior flog entries mentioning the experiment, on the background page for this experiment.

What did we do in summer 2022?

Team Echinacea was busy this summer! Over the last few weeks, we have been posting updates on projects from summer 2022. Here is the complete list.

Experiments with 2022 updates:

2022 Update: Aphid addition and exclusion

In summer 2022, Team Echinacea continued the aphid addition and exclusion experiment started in 2011 by Katherine Muller. The original experiment included 100 plants selected from exPt01 which were each assigned to have aphids either added or excluded through multiple years. The intention is to assess the impact of the specialist herbivore Aphis echinaceae on Echinacea fitness.

In 2022, Emma Reineke and Kennedy Porter conducted the aphid addition and exclusion project. They located 24 living exclusion plants and 17 living addition plants. Similar to the past two years, they did not find any aphids in exPt01, so they started to introduce a new population of Aphid echinaeceae into ExPt1. Learn more in the summer aphid update.

  • Start year: 2011
  • Location: Experimental Plot 1
  • Overlaps with: Phenology and fitness in P1
  • Data collected: 
    • Plant status (basal, flowering, not present), aphids present, ants present, herbivory (number of leaves significantly chewed on), and the number of aphids added/removed (depending on specific treatment)
    • Protocols and datasheets are located at ~Dropbox\aphidAddEx\aphids2022
  • Samples collected: NA
  • Products:
    • Andy Hoyt’s poster presented at the Fall 2018 Research Symposium at Carleton College
    • 2016 paper by Katherine Muller and Stuart on aphids and foliar herbivory damage on Echinacea
    • 2015 paper by Ruth Shaw and Stuart on fitness and demographic consequences of aphid loads

You can read more about the aphid addition and exclusion experiment, as well as links to prior flog entries mentioning the experiment, on the background page for this experiment.

2022 Update: Dust experiment

In summer 2021, Team Dust began a project to look at the effect of dust on reproduction of Echinacea. They randomly assigned treatments of ‘dust’ or ‘no dust’ to 41 heads in ExPt2. Many heads were eaten by ground squirrels, but they harvested the 18 survivors, and Amy Waananen x-rayed the achenes from these heads in March 2022 to evaluate seed set.

This summer, Emma Reineke took the lead on the project, assisted by Kennedy Porter. They applied dust treatments to 64 Echinacea heads at a prairie remnant, Nice Island. They also conducted an observational study using dust traps to measure dust levels at varying distances from unpaved roads. Learn more in Team Dust’s summer update. In late summer, the team harvested the 64 heads, and Amy now has them at UMN. Emma recently received UROP (undergraduate research opportunities program) funding to continue research on the dust project during spring semester.

  • Start year: 2021
  • Location: ExPt2 and Nice Island
  • Overlaps with: None
  • Data collected:
    • Relative amounts of dust levels along unpaved roads at Aanenson and Riley
    • Datasheets are located at ~Dropbox\teamEchinacea2022\emmaReineke\Dust 2022
  • Samples or specimens collected:
    • We collected 64 heads, which are currently in the R. Shaw Lab in the Ecology building at UMN.
    • The dust traps and dusty styles are also at UMN.
  • Products: Amy presented Team Dust’s work at The Prairie Enthusiasts (TPE) conference in February 2022.

You can read past flog entries about the dust experiment here.

2022 Update: Demographic census in remnants

Since 1995, the Echinacea Project has been mapping and collecting demographic information on Echinacea angustifolia to generate detailed, long-term records of individual fitness in prairie remnants. In summer 2022, Team Echinacea visited 34 prairie remnants to search at 2927 locations where adult Echinacea plants had been previously mapped, a process we call “total demo.” At small sites, the team searched for all adult plants, and at large sites, we visited a subset of the adult plants. This year, we did not visit plants that had been “not present” for the past 4 years. However, we added plants that flowered for the first time in 2019, 2020, or 2021. At the large sites, we added many more plants than we removed; at Landfill, we removed 18 plants but added 129 new plants, so we visited 285 plants in total. We plan to revisit the total demo protocol before next summer so our subsets do not reach unreasonable sizes.

At each Echinacea plant, the team used handheld data collectors (visors) to record the flowering status, number of flowering heads, number of rosettes, and near neighbors of the plant. We then mapped the location of every flowering plant within each prairie remnant using a high-precision GPS unit. Unfortunately, the new GPS unit, Collins, stopped working early in the summer. Nevertheless, the team persisted with the old GPS.

Johanna and Kennedy collect demographic data on Echinacea at On27

In summer 2022, Team Echinacea collected 7926 demographic records (demo) and recorded 3708 GPS points (surv). In total, we collected data on ~2870 flowering Echinacea angustifolia plants. A combination of favorable weather and prescribed burns made it a record-breaking year for flowering Echinacea. Landfill had 713 flowering plants this year (east: 373, west: 340) compared to only 327 last year. There were 518 flowering plants at Loeffler’s Corner (east: 292, west: 226), and 266 flowering plants at Staffanson. The demo and surv datasets are in the process of being combined with previous years’ records of flowering plants in “demap,” the spatial dataset of remnant reproductive fitness that the Echinacea Project maintains.

This year, new tags ranged from 27001 to 27999. However, due to the high number of flowering plants and issues with people adding unnecessary tags, we ran out of tag numbers in the 27000s. Therefore, we also used new tags ranging from 25651 to 25890 and from 26881 to 26999 in 2022.

We are especially interested in understanding how fire influences reproductive effort in fragmented prairies. The following sites were burned in spring 2022: Bill Thom’s Gate, Landfill West, Loeffler’s Corner West, Martinson’s Approach, Northwest of Landfill, North of Northwest Landfill, Staffanson West, and West of Aanenson. We noticed increases in flowering at most burned sites, even some of the small ones. For example, West of Aanenson had 7 flowering plants this year, which is the highest flowering rate on record since we started mapping Echinacea there in 1999. Stay tuned for more results from this intriguing dataset!

Members of Team Echinacea do total demo at the Transplant Plot
  • Start year: 1995
  • Location: Remnant prairie populations of the purple coneflower, Echinacea angustifolia, in Douglas County, MN. Sites are located between roadsides and fields, in railroad margins, on private land, and in protected natural areas.
    • Total demo: Bill Thom’s Gate, Common Garden, Dog, East of Town Hall, Golf Course, Martinson’s Approach, Near Pallida, Nessman, North of Golf Course, Randt, South of Golf Course, Sign, Town Hall, Tower, Transplant Plot, West of Aanenson, Woody’s, Yellow Orchid Hill, plus the recruitment plots REL, RHE, RHP, RHS, RHX, RKE, RKW
    • Annual sample: Aanenson, Around Landfill, East Elk Lake Road, East Riley, KJ’s, Krusemarks, Loeffler’s Corner, Landfill, North of Railroad Crossing, Northwest of Landfill and North of Northwest of Landfill (lumped), On 27, Riley, Railroad Crossing, Steven’s Approach, Staffanson Prairie
  • Overlaps with: Flowering phenology in remnantsreproductive fitness in remnantsEA fire and fitnessfire and flowering at SPP
  • Data collected:
    • Plant status (can’t find, basal, dead this year’s leaves, dead last year’s leaves, flowering), number of rosettes, nearest neighbors, and head count, if flowering
    • All GPS files are found here: Dropbox/geospatialDataBackup2022
    • All demo and surv records are stored in the aiisummer2022 repo
    • The most recent copies of allDemoDemo.RData and allSurv.RData can be accessed at Dropbox/demapSupplements/demapInputFiles
  • Samples or specimens collected: NA
  • Products:
    • Amy Dykstra’s dissertation included matrix projection modeling using demographic data
    • The “demap” project is a long-term dataset that combines phenological, spatial and demographic data for remnant plants

You can read more about the demographic census in the remnants, as well as links to prior flog entries about this experiment, on the background page for this experiment.