Categories

up close of Echinacea floral structure

Look at this paper to see some nice photos of Echinacea floral parts (Wist and Davis 2008).

potential sites to assess Competition for Pollinators

Here’s a file that lists sites you can choose for your study.

Also, here’s a list of equipment that we used for during the first field season where we did systematic observations and collections of pollinators.

Allegra’s options

Here’s a file with plants that Allegra can use for her pollen interference/precedence experiment. These are all plants identified yesterday as going to flower in 2009. Plants are sorted according to priority order–random, except that plants from site of origin “Unknown” are given priority 1.

I recommend going down the list and excluding all plants that don’t have at least two promising heads. Flag all included plants with labels 1, 2, 3, …. Stop when you have enough for your experiment. Ask Gretel which color flag to use. Blue is an option because your plants are in CG96 and Andrea’s are in CG97.

pops for sampling tissue

Here’s Jennifer’s preliminary list of sites. She wants a total of ten sites and wants to sample from all that are asterisked.

Populations for sampling
These are pops I used for looking at long term flowering in the CG
The pops with * are the ones DR looked at in Dec with Fst values

Aa
Alf
Eri*
Kj
LC
Lf*
Ness*
Nwlf*
Riley
RRX
Spp*
Stevens*

wanted: digital camera for microscopes

We have several microscopes and we would like to capture digital images of what we see–especially pollen grains. Which on should we pick? Any advice would be appreciated. Here are some links to pages about several models of inexpensive USB cameras that can mount to a microscope:

microsope.com sells several brand. They sell only one line from bigC.

Here’s a company that sells the EM series from bigC.

documentation pages on the bigC product line (more on the AM series than the EM series).

Another source of info on the Moticam line.

I scanned this list of companies that sell (and used to sell) microscope accessories to find the above links.

FYI I stumbled across some pollen identification keys: a taxonomic list, a key to pollen of the bahamas, and an inaccessible “pollen database” that sounds good.

find spittlebug spittle masses

This file lists places to look for spittlebug spittle masses in the CG. At the top there are rows in the 99 garden and the 99S garden sorted randomly. Then all bigbatch rows (noted with end positions) are listed in a random order.

Enjoy your search!

Week 3 plans

Lots of plans for this week! Here are some highlights.

This week we will start systematic observations of Echinacea flowering phenology in the CG experiment. We want to know the first and last day of flowering for every head of every Echinacea plant in the CG. The main event early in the week will be to put a twist tie on every head that looks like it will flower. We will also put a flag near every flowering plant with its location on the flag. We have to get the locations (plant ids) correct and get it into a database. As of Sunday, four plants in the CG had started to flower, how many left to go? We will also record flowering phenology at Staffanson Prairie Preserve. We will observe many fewer plants, but it’s a long walk.

Under the supervision of DR, we will spend ~1h looking for more spittle masses on Ea in the CG.

Jennifer and Diedre are coming from IL this Sunday and will stay for the week. They will help set up the phenology flags. They also plan to collect tissue from plants in several remnants to do a population genetic study using microsatellites (DNA markers).

Daniel and Amy will make a plan for searching for aphids and juvenile plants in remnants.

Caroline will fill us in on her plans.

The competition of pollinators crew (M “floral neighborhoods” J, A “bee’s knees” G, K “style” G, A “the experimenter” H, and G “pollen from the source” D) will plan and practice for their project. Here are some things they will do…
Mimi: characterize floral neighborhoods
Amanda: catch bees, get pollen on slide
Kate: catch styles, get pollen on slide
Allegra: choose plants for experiment
Greg: order digital microscope cam & collect pollen (from the source)

GPS (maybe): Daniel & Amy.

What are we going to do about that tripod?

Week 2

Team Echinacea had a busy week last week.

We finished seedling searches in the remnants.

We found ~22 spittle bugs masses in the CG.

We talked a lot about plans for many projects and started organizing and practicing.

We ordered supplies.

On Thursday afternoon we pulled and cut thistles in the CG.

We started and finished the “recruitment experiment.” This experiment started off as the “recruitment experiment.” I hand broadcast seeds in fall ’00, ’01, and ’02 in plots with different burn treatments. Now we are assessing plant survival. (We need a better name for this experiment.) Last year there were over 820 plants alive. After a quick scan of the datasheets, I think 9 plants will flower this year. Wow, much less than 1%! These plants are taking a long time to flower. After entering data, Amy will give a detailed summary of our findings this year. Notes for next year: improve datasheets for entering fl pla info, avoid searching at empty spots, & map plants using tripod system.

On Friday the first plant in the CG started to flower — one floret started male phase. We saw the pollen. No plants started on Saturday and on Sunday 3 plants started to flower.

Seedling searches finished!

We finished searching for seedlings at the last site (Staffanson Prairie Preserve) on Monday. All the datasheets & maps (163 pages) are now organized in a 3-ring binder.

Here are a few highlights:

We found total of …
> 22+1+5+1+8+2+24+4+13+0+5+7+1+0
[1] 93
… ninety-three seedlings at fourteen sites!

In August we’ll go back and check the fate of every one of those seedlings. I hope we can find them all!
IMG_9234.JPG

Mimi, Amanda, Greg, Allegra, Daniel, Caroline, and Gretel
looking for seedlings on the scraped roadside at Riley’s site.
(They didn’t find any here.)

Two possible Echinacea seedlings (not counted above) were noted. We should go back to check their identity within the next week. At site NWLF we left a pin flag at focal plant #13073. At site ERI the possible Echinacea seedling was at R102 (see page 97). Help me remember to check these!

We found about 500 other Echinacea plants within the circles, mostly juvenile plants and some adults (flowering and not).
> 16+16+25+131+63+33+73+24+46+5+16+46+6+11
[1] 511

The roadsides at sites ER and ERI were scraped. In the area that was scraped, all the tags are gone. We did see many little Echinacea leaves peeking through the gravel, but no seedlings. In some areas the scraping was deeper and some roots of old plants were pulled out. I collected one pulled root from the S side of the road on the W half of RI; I couldn’t tell from where it was yanked. IMG_8873.JPG

The root was huge!

With our very precise maps of plants from previous years, we will be able to identify which plants are gone and which persist. It will be a challenge though. In some dense areas we may not be able to figure it out. Stay tuned, we’ll bring the detailed maps and try to figure it all out in August, after peak flowering.

IMG_9222.JPG

Gretel determining the identity of individual Echinacea plants
at the scraped roadside at Riley’s.

The scraped gravel was piled in the ditches. Some plants in the ditches were buried and I expect that many of them will die. There will probably be a lot of weeds in and around those piles for the next few years (until the perennials take over again). IMG_9216.JPG

Two images (above & below) of the piles of gravel deposited
in the ditch on the S side of the road at Riley’s.

IMG_9211.JPG

Another highlight (no photos though):

It was a pleasure to visit Staffanson. Gretel and I mapped the focal locations on Sunday and saw a patch of Cypripedium calceolus in flower (past prime). Almost every focal plant in the West unit (unburned) had spittlebug spittle on it. Almost none of the focal plants in the East unit (burned) had spit.

We didn’t use the tripod to take photos. The camera didn’t attach well and the remotetrip feature isn’t ready yet. We’ll need to work on the tripod and practice using it. I think it holds great potential to speed up and improve our protocol.

Kensington Runestone Days

Something to look forward to! I heard that Team Echinacea’s front steps are the best seats in town for the Kensington Runestone Days Parade.

Here’s the schedule of events for this weekend.