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NSF funding to continue

In early December, NSF informed us that The Echinacea Project will be funded from April 2011 – April 2016!

hlh worksheets

allSpotsForPlugs_7July.xls

Plug Datasheet_Verification_07July2010_a.xls

files for planting plugs

plugPlantingInfoForKate.csv

emptySpotsForPlugs.csv

A/D data

Plug Datasheet_Verification_04July2010_a.csv

KG_site&trays.csv

label document

rowPos_envLabels.docx

label info for Kate

This file, labelInfoForKate.csv, is for making new envelope labels.

03 July – row/position R data and csv files

I only need 680 positions/site, because the seeds will be in between the plug points. So attached is a .doc and an R file w/ the script to create 3 sites with ~680 positions in each. I have also attached the resulting .csv file, 3 columns “site”, “row”, and “pos”.

KG_row&pos_03 July.doc

KG_row&pos_03 July.R

KG_positions_03July.csv

Here’s the breakdown:
site breakdown.xls

Next steps:

  1. Assign each new.env ids to a row and position. See file: sane3blocks.csv
  2. Create labels.
  3. Put labels on envelopes.
  4. Assign each plug to a row and position (keeping in mind that they’re already randomized in the trays.)
  5. Develop planting protocol.
  6. Organize materials for planting.
  7. Mow sites.
  8. Plant.

July 2nd: Data for new labels

For creating new labels:
KG_newLabelData_02July.csv

KG_Purchased Seed EnvelopesWS.csv

Plug Datasheet_D-A_02July2010.csv

For verifying status of plugs:
Plug Datasheet_Verification_02July2010.xls

summer schedule 2010

We’re off to a great start this season. We’ve made good progress on our ongoing projects and folks are well on their way with their independent projects. We had better keep moving because the earliest plant in the Common Garden started flowering on the 21st!

Here’s a list of independent projects for Summer 2010:

  • Laura: Phenology of midsummer prairie plants
  • Josh: Movement of Stipa spartea seeds
  • Lauren & Hillary: Performance of aphids on Echinacea and other plants
  • Katie: Efficiency of common Echincaea pollinators
  • Ian: Flowering phenology and mating compatibility
  • Greg: Breeding systems in the Asteraceae
  • Gretel: Reciprocal pollen interference between Heliopsis and Echinacea

I attached a pdf file of our ongoing projects.

Greetings from the Lab…

Hi, Team Echinacea —

Its Diedre and Jake with an update from the lab at the Chicago Botanic Garden.

In spite of several setbacks, including a crowded lab and a power failure that shut the lab down for an entire day, we’ve been able to create a lot of data. Currently, we have ten microsatellite primers working which we use for paternity and genetic diversity analysis.

Recently we’ve been able to up our extractions to over 150 samples a week and 10 PCR’s a day! Jake and I started extracting the samples that Jennifer and I took in Minnesota several weeks ago. Jake is using these for his poster on population structure. He will be looking at whether there is interbreeding or inbreeding among the nine remenant populations we sampled (East of Riley, Anenson, Steven’s Approach, Landfill, Railroad Crossing, Staphenson Prairie Preserve, KJ, and Ness). Jake has already found some interesting results with the use of Structure and FStat.

The poster I am working on will look at the diversity of pollen donors with regard to flowering on individual and population levels.

Here are some pictures of our work in the lab:

Jake with Sculpture.JPG

Pippette Sculpture.GIF

Jake and I making DNA extractions a little more fun than they already are through use of our artistic talents.

Picture of Exciting Results.JPG

Some preliminary results for Jake’s project.