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Hybrid seedling

What’s this in plug 156? A young seedling with fused cotyledons and a true leaf just peeping up. in the nearby corner is a more typical seedling. Both plants come from florets of Echinacea angustifolia that were pollinated with pollen from Echinacea pallida.

hybridPlug156Wednesday2012May16.JPG

Click image to embiggen!

Fire and patience help when establishing Echinacea in a prairie restoration

In a paper just published in Restoration Ecology, Echinacea Project researchers report that establishing Echinacea angustifolia in existing prairie restorations and abandoned agricultural fields requires more than 20 seeds for each plant that germinates and survives to flowering. Plants start flowering about 10 years after sowing. Also, burning the prairie before broadcasting seeds helps emergence and survival.

Wagenius, S., A. B. Dykstra, C. E. Ridley, and R. G. Shaw. 2012. Seedling recruitment in the long-lived perennial, Echinacea angustifolia: a 10-year experiment. Restoration Ecology 20: 352-359. Available here: https://echinaceaproject.org/pub/wageniusEtAl2012.pdf

MEEC poster, awn edition

meec_poster-page001.png

Click to embiggen the poster. This isn’t the full poster, but I’m not uploading a 30MB PDF. The videos can be read with a smartphone and a QR reader, or flip through my posts, as all the videos are on the flog somewhere.

Another poster!

I couldn’t make the Midwest Ecology and Evolution Conference, but I made a poster. It describes preliminary results from an aphid addition/exclusion experiment I conducted in the summer of 2011. Specifically, it examines the question of whether aphid infestation influences the presence of leaf damage by other herbivores.

MEECPosterKMullerFinal.pdf

Dichanthelium Update!

Hi everyone!

Maria here. Sorry that I have not posted since the end of summer, but please rest assured that I’ve not run away with my Dichanthelium seeds, but have been working on them for the past -what? 6 months? A long and intimate relationship indeed.

Brief summary of what has happened:

I did a pilot germination & growth study using bulk Dichanthelium seeds. The results of germination study is nicely summarized in this poster that I presented at Midwest Ecology and Evolution Conference (MEEC) in Cincinnati 2 weekends ago(?). MEEC was fun and presenting (yapping about) my poster was a lot less nerve-wracking than I had expected:
MWangMEEC2012poster_44_x_36.ppt
Thanks to everyone who helped me in my hectic rush to get the poster done X_X

The seedlings are currently growing in the growth chamber at CBG. (There’s pictures in the poster of seedlings in agar and in plug trays!)

I shall put up some more pictures sometime in the future.
There’s a series of pictures I want to put up showing seeds before and after x-ray and scarification – it’s pretty interesting.

I should also post the R script I used to analyze data and produce the graphs on the flog – unfortunately don’t have the file on this computer.

Right now I’m working on scarifying Dichanthelium seeds for my maternal lines growth and germination experiment (probably should explain in better detail later, likely in another poster).

Other good news you might find interesting:
Thanks to A LOT of help from Stuart and other advisers, I applied and got the Northwestern Academic Year Undergraduate Research Grant for my Dichanthelium project during the school year (maternal line germination/growth experiment), and also very recently, the Garden Club of America Clara Carter Higgins Summer Environmental Study Scholarship =)

If you have any questions about Dichanthelium or anything I talked about, you’re welcome to get in touch. My email is right under the entry title.

2012 Planned Common Garden Measuring Protocol

Here is the protocol that we plan to use for measuring in 2012:
2012.measureFieldProtocolPlan.htm

2011 Common Garden Measuring Protocol

Here’s a link to the protocol that we used for measuring CG1 in 2011:
2011.measureFieldProtocolReal.htm

In 2012 we plan to measure in “review mode” (as we did for CG2 in 2011) — all location records will be on the Visors with Status=”Staple” or “Skip” populated. We should not spend as much time searching for plants that have not been present for 3 or more years.This should speed up measuring. I’ll post the planned 2012 protocol next.

Heads in CG1

Now that we’ve inventoried all the CG1 heads, I checked to see just how many we have. There were about 3009 twist-ties put out, and 119 heads were duds or missing, so our (estimated) total number of good heads is about 2890.

CG2 had something like 140 heads, but we haven’t inventoried those yet.

flowering in 2011

Flowering of Echinacea angustifolia in almost all prairie remnants was down this year. Overall, approximately half as many plants flowered this year as last. Two areas distinctly bucked the trend: flowering was high at Hegg Lake WMA, which was burned this spring, and at our main experimental plot, which was burned this spring. Burning really encourages flowering!

We finished our first round of mapping all flowering plants in nearby remnants and a summary of the raw dataset is shown below. Each line lists the name of a site and the count of demo records and survey records at the site–also the difference in counts. We call our visits to remnants to find and refind plants “demography,” or demo for short. We call mapping the plants surveying because we used to use a survey station. Now we use a survey-grade RTK GPS (a Topcon GRS-1).

      site demo surv diff
1        x    1    0    1
2       aa  131  103   28
3      alf   79   52   27
4      btg    8    3    5
5       cg   20    5   15
6      dog    4    2    2
7     eelr   60   44   16
8      eri  153  122   31
9      eth    9    3    6
10      gc    7    1    6
11      kj   61   44   17
12    krus   69   21   48
13      lc    0    0    0
14     lce   58   45   13
15     lcw   48   31   17
16      lf    0    0    0
17     lfe   77  117  -40
18     lfw   65    0   65
19     lih    2    0    2
20    mapp    5    3    2
21    ness    7    3    4
22     ngc   28   12   16
23   nnwlf   20    7   13
24    nrrx   42   27   15
25    nwlf   27   10   17
26    on27   71   85  -14
27      ri  241  210   31
28     rlr    0    0    0
29    rndt   10    2    8
30     rrx   70   51   19
31   rrxdc    4    0    4
32     sap   80   38   42
33     sgc   10    4    6
34    sign    0    0    0
35     spp  126   78   48
36      th   19   12    7
37   tower   10    3    7
38 unknown    8    0    8
39     waa   10    6    4
40    wood   33   21   12
41     yoh   23    8   15

Notice that most sites have more demo records than survey records. This is because each data recorder enters an empty record at the beginning and end of demoing a site. Also, in certain circumstances we do demo on non-flowering plants.

Something strange is going on with the on27 site. I think someone may have entered the incorrect site name when doing demo. Also, lf looks strange, but is easily explained: lf is divided into two hills (lfe and lfw). We distinguished the two when doing demo, but not when surveying. Our next field activity is to verify the demo and survey dataset and make sure everything makes sense. Being people, we sometimes make mistakes in data entry. Because we know we make mistakes, we generate two separate datasets of flowering records (demo and surv) and compare them. When records don’t match, we go back and check.

We assess survival and reproduction of Echinacea plants in remnants to understand the population dynamics of these remnant populations. We want to know if the populations are growing, holding their own, or shrinking. To figure this out will take a few years because plants live a long time. Estimating a population’s growth trajectory based on just a couple of years of flowering records probably won’t be that informative.

Project Status: Dichanthelium & Compatophen

After a good 3 months of sunshine and storms and flower-counting, it’s time to head back to civilization and school. Here are my project status updates and associated files. The doc files (MWang_Dichanthelium.doc and MWang_Compatophen.doc) explain what the associated documents are. Some files (perhaps older versions) can be found on the shared drive.

Dichanthelium:
ProjectStatusMWang_Dichanthelium.doc
MWang_Dichanthelium.doc
Dichanthelium_Protocol_FieldMethods.doc
Dichant_DE_All_2Sep2011.xls
Dichant_ReturnsSummary.xls
Dichant_ReturnsDatasheet.xls

Scanned datasheets that don’t really have much information:
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CompatoPhen:
ProjectStatus_MWangCompatophen.doc
MWang_Compatophen.doc
Compatophen_PrelimAnalysis.xls
Compatophen_SamplingCheck.xlsx

I will be continuing work on my projects in the fall.