This week, I have decided on my independent project for the rest of my internship! I will be working with Lilium philadelphicum, wood lily and investigating questions surrounding its pollination and reproduction. I felt more informed on the past research by the Echinacea Project after hearing presentations from Stuart and Jared. Jared further taught me the benefits of fire on prairie ecosystems. Prairie plants are fire dependent and thrive after burns. This is due to the natural landscape and indigenous traditions. It was very interesting to learn about the natural history of our region and how plants grew before impacts of modernization. Results from past research in the lab has shown benefits from prescribed burns on the reproduction of echinacea. This applied conservation method could potentially benefit other fire dependent prairie plants as well! I hope to observe patterns and variation of pollination success in L. philadelphicum. I am currently developing a hypothesis for this project. Later on I will analyze the data set collected by Jared over the summer.
We have already completed inventory of the data set and started on the cleaning process of L. philadelphicum. The fruits of L. philadelphicum can be seen in the picture below. It contains many seeds, some of which are large and dark, others small and lighter in color. We hope to find interesting information through the variation of these seeds. Stay tuned for next week, as I will be working on developing the protocol for cleaning, scanning and counting these seeds.
The fruit/pod of wood lily contains three separate chambers stacked with seeds.
So far this year, we have sadly not been able to have volunteers in the lab due to the continuing threat of COVID-19. However, over the last few months, we made quite a bit of progress on the remnant Echinacea harvests from 2020 and 2021. In the fall, we had help from volunteers, students from Lake Forest College, and externs from Carleton College. Thanks for your help! In January, Sophia finished cleaning the last head from 2021, which was an exciting accomplishment.
To track our progress in the lab, I created an R script to visualize the various steps of the ACE process for each batch of Echinacea. The figures for rem2020 and rem2021 are included here. Hopefully, this method will work for the cg harvests as well.
Click on the figures to enlarge
The ACE stages are listed along the x-axis, and the number of Echinacea heads are on the y-axis. The light blue shows how much we have completed, and the dark blue shows what remains to be done. The small numbers on each bar indicate the corresponding number of heads, and the width of the bars is roughly proportional to the amount of time each step takes. Along the top, the dates indicate the last day that the totals for each stage were updated.
The script to create these graphs can be found here: echinaceasandbox/oop/trackAceProgressTest.R
Today is the last day of the externship and we presented our findings.
After a really intense afternoon of learning to use R and working with data on Thursday, we made some graphs and did some statistical tests on the data we have been collecting (as well as spatial data that Jared and Alex got ready for us). It was really hard because I didn’t have that much experience in R, but it was really rewarding as I learned how to make the graphs I want and do statistical tests.
I was interested in looking at tradeoffs plants make in reproduction in the first place, but then I realized that I couldn’t directly quantify resources plants put into different aspects of reproduction with the data I had. I decided to change things a bit and focus on limitations to reproduction. I set up scenarios of plant’s reproductive effort under different conditions in terms of resources and tried to clearly explain it by having hypotheses and visual aids. Another potential limitation I considered was limitation in pollination, which I quantified by looking at level of isolation based on distance to neighbors. I made a lot of assumptions which I hope are true – for example that plants really would put all the resources into reproduction, and that distances from other conspecific plants are a predictor of pollination.
I didn’t find strong evidence for any of my hypotheses except that there is a negative relationship between the distance to 3rd nearest neighbor and seed set in liatris. It was interesting but also expected – isolation seems to be negatively impacting pollination and thus seed set.
The presentation went well and these are the slides I used for it.
Overall, this externship has been a really great experience. I learned so much about the processes of doing science, about what scientists do on a daily basis and how a research group functions. I’m really glad that I got to participate in the lab meeting, explore in the Garden, be a part of many processes of research and eating lunch with the lab group everyday!
I also learned that studying plants is a lot harder than I imagined. It’s a really long process, and there are many biases that could happen. Organizing a lab takes a lot of work, and making a standardized ‘assembly line’ to process hundreds of plants every year is incredibly hard. The results or findings sometimes are not what you expected, or don’t seem to make sense at all. These are all things that I never imagined to be a part of studying plants and ecology. I feel like now I have a much better idea of what the field is like and what to expect moving forward from here. I am really grateful for this experience and for everyone who made it possible.
I can’t believe our externship is almost over! Just yesterday we finished randomizing Liatris, so today we started randomizing Echinacea. The procedures were similar, except we used a circle (which looks like a carnival spin-the-wheel) instead of a grid when scattering achenes. The total achene numbers were also unusually small in 2020 and 2021 compared to the previous Echinacea collection years, so we actually had to alter the randomization protocol in order to get a minimum of 25 achenes that can be classified for pollination.
Here’s me, Cassie, and Wanying randomizing Echinacea by scattering the achenes on the circle (lower left) and using the letter randomizer on the project website to select achenes to be put onto the counting sheet (right of the circle)
We also x-rayed the rest of the Liatris today and learned how to classify achenes that have been x-rayed. These x-rays are meant to look at the seed status of the achenes in order to determine which achenes were pollinated. We used the Echinacea classification training module on the Echinacea Project website and created specific criteria for Liatris in order to mark the achenes as empty, partial, and full. We should be able to finish x-raying and classifying the Echinacea we randomized tomorrow morning!
The achene classification training module, which includes an interactive practice classifying session
However, the best part of my day was meeting with one of the Ph.D. students doing research in the lab, Drake, and learning about their current work with parasitic plants. Drake and Lea, who is another Ph.D. student that we met earlier in the week, gave valuable insight about preparing for grad school and Ph.D. programs. They especially gave great advice about not being afraid to cold email people we want to connect with as well as how to take advantage of our unscheduled time for research, especially in the latter Ph.D. program years.
I’m also excited for tomorrow, which is when we will finally get all of our data processed! With this data, we’ll be able to answer our research questions and input the results into our presentation for Friday’s lab meeting. I can’t wait to not just get the results, but also to discuss them and their implications with the project team!
Alondra, Connor, Maeve, and Marina finished their mini-internships with us. It was a great experience for them and us. We appreciate their contributions to science and conservation and they gained valuable experience. As part of their plant biology class, Alondra, Connor, Maeve, and Marina, who are juniors and seniors at Lake Forest College, worked on two projects to assess effects of prescribed fires on reproduction in Echinacea. In the lab, they gained hands-on experience in seed biology over three Wednesday afternoons, including cleaning, scanning, counting, developing hypotheses, and data management. To test their hypotheses, they developed a dataset and summarized their results. In class they presented posters and they are attached here. It was a wonderful mini-internship–thanks to Alondra, Connor, Maeve, and Marina, as well as Prof. Westley!
The second week of the externship is coming to an end. We started the day by finishing off all the counting we needed for our final project. We counted achenes of echinacea heads harvested in 2020 and 2021 from several burnt and unburnt places. The echinacea plants these heads came from also had variation in density and number of heads produced so that the data we generated from counting would be helpful for all of our projects.
Caitlin and Cassie counting echinacea achenes
We had our weekly lab meeting at 9 and talked about a paper Jared had been working on. I learned more about the process of scientific writing and it was inspiring to learn that studies of one particular plants could provide insight into methodology used in ecological research in general.
Later in the afternoon, we had a brainstorming session on preparing for our presentation next Friday. We all discussed our hypotheses, potential graphs, diagrams and pictures to include in a slideshow. We all felt more confident about it and were excited to see what we could find out next week!
This week we’ve been making good progress on gathering the necessary data for our projects! An important part of this, especially for mine and Wanying’s projects, has been outlining our randomization protocol for liatris. After brainstorming a liatris randomization protocol yesterday to ensure we would be getting unbiased samples for our data, today we finalized some of the details of our protocol and started randomizing!
We spent the majority of the day creating random samples of liatris achenes to use for our projects, and we were able to get 60 samples done. It was exciting to put a protocol that we had drafted into practice, and continue working towards our data sets and the final product of our projects. The variation of achenes from each plant was also very apparent during the randomization process. Here’s Wanying, Caitlin, and Wyatt working on randomized liatris samples:
We finished off the day with some echinacea achene counting, which will be important for Caitlin’s data analysis. It’s fun to get a variety of different tasks throughout the day while contributing to different projects. I’m looking forward to more randomizing tomorrow and doing more work on our projects!
We’re now past the midpoint of our externship! This week we are focusing more on gathering the data for our own projects, so yesterday Alex and Mia gave us a list of Echinacea purpurea samples that would be helpful to use in my project. Cassie, Wanying, and I separated these samples and moved them through the entire process we had learned so far, including cleaning, rechecking, random letter-number label assigning, reordering, and scanning. Thanks to this work, we only had a few scans to do today and redid the scans that did not have any achenes so that they now include a note to any potential counters.
Here I am adding notes to the achene-less scans, which are done on the right and entered into the computer program
Then we started thinking about Liatris aspera, which is used in Cassie and Wanying’s projects, and how to randomize each plant’s achenes in order to get smaller samples that can be realistically checked for seed predation and x-rayed. We learned the Echinacea randomization protocol today, but there is no protocol for Liatris, so we brainstormed one together based on the challenges of Liatris and our goals. We started to draft this protocol into a document listing our objectives, materials, troubleshooting tips, and methods. Then we cleaned Liatris until the end of the day, and I made endless more grub friends.
Wanying and Cassie cleaning Liatris headsA grub just chilling and pretending it didn’t eat through the achenes I needed
I’m excited for tomorrow, which is when we’ll hopefully be able to start randomizing and maybe even putting our protocol to the test!
This Friday was day 5 of our externship and we had plenty of new experiences! We started the day by learning how to cleaning a different flowering prairie plant, liatris, or rough blazing star, with Wyatt. Compared to echinacea, achenes of liatris are much easier to be separated from the chaff and the receptacle. However, the fruits of the liatris are spread by wind. So the achenes all have fluffs attached to them which is a little challenging to separate sometimes.
Cleaning liatris!
Then we participated in the echinacea project’s weekly lab meeting. We read the draft of a paper Lea had been working on and listened to lab members discussing it. It was really fun and inspiring to see the process of scientific writing and experience the lab as such a collaborative space. I learned a lot about the behind the scene processes in doing science such as designing experiments, deciding on certain sets of data to use for a given topic and different ways to present data.
Right after the lab meeting, we listened to speed talks given by ecologists working in different departments of the Garden including aquatics, remnant forests and some other natural areas. I learned a lot about the history of the Garden as well as challenges and successes in managing different ecosystems within the Garden. I was really surprised when I learned how much work went into maintaining the lake area and the shores of lakes – planting, reinforcing, cleaning algae and other unwanted aquatic plants and so on. It was a great experience listening to talks by ecologists doing hands-on restoration and conservation work here in the Garden.
We also learned the ABT format of presenting a project and all three of us tried to come up with one for our projects here. We also decided on what we’ll be doing for projects. Cassie would be looking at density and seed set in liatris, Caitlin fire and seed set in echinacea, and I would be looking at number of flower heads and seed set in liatris. We planned to do some cleaning, rechecking, scanning, counting and X-raying to get part of the data we need and then do some data analysis. With this in mind, we were much more motivated to do some more cleaning and rechecking and dive into the second week of our externship!
Caitlin (left) and Cassie (right) focused on rechecking echinacea
As the first week of our externship is coming to a close, we’re learning more about the different procedures and tools used in the lab. After finishing up our rechecking and organizing of the 2020 harvest yesterday, we began scanning today so that we can eventually move on to the next step and count achenes. While we scanned, we also took turns continuing the daunting task of rechecking the cleaned 2021 harvest. Here’s Wanying and Caitlin working on rechecking while I scanned:
As fun as scanning and rechecking is, the most exciting part of my day was easily getting to watch a prescribed burn on some of the restored prairie. While the fire was pretty slow and patchy for the most part, it picked up speed during the tall grass sections and was pretty cool to watch overall. Getting to actually see a prescribed burn in practice after learning about it and its effects in the lab was really interesting. It was also a pretty valuable experience in that we got to watch and learn about how prescribed burns are done and the different tasks and precautions that go into the process.
Some of the patchy burn at its early stages.
After watching the burn and doing some more rechecking and scanning, we had a meeting with the lab team to brainstorm for the independent projects we’ll be working on for the last two weeks of our externship. We talked about different possible project ideas and questions we could investigate, but struggled some with the feasibility of accomplishing our goals in just two short weeks. However, after some more brainstorming and reevaluating, we were able to come up with some exciting ecological questions to investigate in the lab for the next couple of weeks!
Overall, this week has brought lots of new knowledge about the lab processes and the various projects they contribute to, as well as some great background and context for thinking about goals and ideas for the rest of the externship!
