Good news! We managed to get through randomizing the first batch! Now we are working our way through the second batch, and so far, things have been going pretty well in terms of pace and efficiency for both cleaning and randomization. Overall, we have seen a considerably wide range of head counts in the Liatris plants, but predation rates have been relatively low compared to what was observed in 2021’s data. Who knows what we will find in the next several batches, but I am pretty excited about the progress made so far and the results we may see. Hopefully, we will complete the next batch within the next few weeks, if not sooner.
So far, things are going well in the randomization step, but there is still quite a ways to go to complete the first batch of Liatris plants. However, we have counted a considerable number of achenes that have been eaten, and we are starting to get a rough estimate of the predation rate per Liatris plant. We are finding that there are, on average, 1 to 3 achenes eaten per plant out of a total of at least 30 randomly selected achenes. This equals approximately a 3 to 10 percent range for what has been found, but we have also found a few that have had either none or many achenes eaten. In light of these findings, I think we are heading in the right direction with the project, but hopefully, we will need to get much more work done to get through all five batches. Hopefully, we will have all the plants cleaned and randomized before the burning season (which starts in April), but it will take a lot to get to that goal within a little over a month’s worth of time. However, the good news is whether or not we meet our goal, there will be more than enough data to work with, and ultimately we can come to conclusions at the end.
In lab today, I started the process of randomization. This step in the project does take some time and patience, but all of it is worthwhile toward reducing bias and collecting achenes that can be x-rayed or identified as eaten. Similar to the randomization of achenes from Echinacea plants, sheets of randomization and counting grids are used. In addition, we also use plastic bags, white envelopes, stickers, tweezers, and a magnifying glass for this procedure. The first step involves pouring the achenes evenly across the randomization grid and ensuring all the achenes are placed in a specific square on the grid without being on the lines. Then using a randomized list of grid coordinates, we go down the list until we hit a coordinate (an example would be “B2”) with achenes present. We then examined each achene in the selected coordinate to see if there was any predation. If less than 30 achenes were counted in the coordinate, we randomly selected another one going down the randomization list. Once we categorized 30 achenes as either eaten or eligible for x-ray, we took our white envelope and plastic bag and put stickers on them. The envelope will store the eaten achenes while the plastic bag will contain the achenes ready to be x-rayed. On the envelope, we write the date, initials, and the quantities of achenes eaten, not eaten, and uninformative. After putting the achenes in their respective containers, we put them in a pile marked “randomized,” and we move on to the next one. As of today, we managed to get through a little over one third of our first batch, so we still have much work to do before proceeding onward.
After cleaning Liatris plants for a week, I am happy to say that the first of five batches are now finished. Now that the first batch is completed, the project’s next step is ready to begin. The process of randomization will be the next step in the project. This step will involve the random selection of achenes from each plant to avoid bias and separation of achenes that are either qualified for being x-rayed or not. Luckily, both groupings will have a role in the project’s studies. X-rayed achenes will inform us about pollination and reproductive outcomes for each head of a Liatris plant. On the other hand, non-x-rayed achenes can be assessed for why they cannot be x-rayed, including what types of damage the achenes have and if any predation occurred toward those achenes. My research question will focus specifically on seed predation, so using the non-x-rayed achenes will be essential. I am still working on a finalized research question relating to seed predation, but seeing the progress made so far has me excited about what will come next in the project and toward finalizing my research question.
I am happy to say that the Liatris Project is off to a good start. After taking inventory of all the Liatris plants this past week, I got to start the cleaning process. A total of 293 Liatris plants have been counted in the inventory, and all have been sorted randomly into 5 different batches. Today, I got to start cleaning the ones in the 1st batch, and while cleaning, I noticed several similarities and differences compared to cleaning Echinacea plants. Overall, I found that Liatris achenes were much easier to extract from the plant than Echinacea achenes, but counting them proved much more challenging. To make things easier, random selection sheets of different numerical ranges were arranged that listed random numbers from left to right down the sheets. Using these sheets, I could randomly pick out a flower head and count the number of achenes associated with that head. I also had to observe if any achenes were missing from each head on a Liatris plant. I recorded the total number of heads per plant and the number of heads with no achenes, some achenes, or all achenes missing. After taking these recordings, I removed all the other achenes present on the Liatris plants and sorted them into an envelope. Any chaff leftover got put into a separate envelope labeled as “chaff.” So far, a handful of plants have been cleaned, but there is still a long way to go.
Today will mark the beginning of a new project that I will conduct analyzing Liatris aspera (Rough Blazing Star). Like with the Echinacea Project, this project will look at reproductive quantities of Liatris and the potential factors for influencing plant reproduction. At the moment, a specific research question is still in the works and the actual project requires some introductory steps that need to be completed. In the lab, I conducted inventory checks for the Liatris plants that have been harvested and made sure their were not any errors in what was taken into inventory. While doing the checking, I had Leah help me make sure everything was accounted for. Trying to do this alone would have been frustrating so I send my absolute thanks for helping me out with this part. As for the next steps in the project, I hope to begin cleaning the Liatris plants next week and start to come up with a potential research question in the near future. Very exciting things to come!
see the “Phases” section about Kuhn’s phases of paradigm change
Discussion questions:
What paradigm shifts have occurred in the fields of ecology and plant science? Have any occurred recently?
Conversely, have you observed new research in the field becoming less disruptive over time? If so, do you agree with the authors’ suggestion that this can be attributed to researchers focusing on “narrower slices of previous work”? (142)
What is your perception of the paper’s measure of disruptiveness (CD5)? What might be some strengths and weaknesses of this approach?
Do the popular media pieces effectively communicate the paper’s findings to a general audience? What angles do they take in their explainers, and why?
Though papers have become less disruptive on average, the quantity of disruptive papers has remained largely consistent. Do you agree with the paper’s proposal of a carrying capacity for highly disruptive papers?
Is a decline in disruptiveness exclusively a bad thing? Consider Kuhn’s ideas on “paradigms.”
After a successful field season, Team Echinacea has moved back to the lab at the Chicago Botanic Garden.
Today, I received a cryptic email from CBG suggesting that there were free items left over from the Roadside Flower Sale, and they were located in Briller Hall, next to the pole barn. Lindsey and I decided to investigate, but we had never heard of Briller Hall or the pole barn. We looked for them on the map of the Garden, but they weren’t listed. We hunted for them on CBG’s website, but there were no search results. Mystified, we asked the volunteers in the lab whether these were real buildings. Neither Allen nor Char had been there, but Allen recognized the names and thought that they were located somewhere nearby. Determined to identify the elusive Roadside Flower Sale, Lindsey and I set off on a quest.
First, we walked south from the lab. We passed the greenhouses and spotted the maintenance sheds, but we didn’t find any pole barns there. How hard could it be to hide a barn? we wondered. When we passed some volunteers who were outside weeding, we asked them if they had heard of Briller Hall. They hadn’t, but they directed us to a CBG worker named Javier who was zipping by on his golf cart. Javier exclaimed that he was on his way to the pole barn just then! He invited us to hop on, so we crammed into the golf cart, and he dropped us off at the secret Briller Hall.
Inside, we found a wide array of marvelous items ranging from bags of acorns to stacks of staplers to a single wooden shoe. We collected many useful items, some for personal craft projects and some for the lab. Our glorious horde included:
5-gallon buckets with lids (they smell rather strongly of lavender potpourri)
duct tape in at least 7 vibrant colors
a sturdy rake for Jared to clear burn breaks
sharp scissors
small notebooks for the summer team’s pouches
a giant hot glue gun for Lindsey’s craft projects
I celebrate the many goodies we found at the top-secret Briller Hall
Overall, we were quite pleased with the success of our reconnaissance mission, and the lab now has a grand supply of staplers, colorful cardstock, and thumb tacks.
It’s been a busy spring at the lab: 11 volunteers and 6 students from Northwestern and Lake Forest College contributed to the Echinacea Project. We are currently wrapping up before field season starts, and we want to celebrate everything they accomplished in the last few months! Since January, volunteers and students:
Here is a recap of the Orchid Show if you missed it this year! The annual orchid show at the Chicago Botanic Garden displays a wide collection of orchids. This event celebrates the beginning of spring with the unique and vibrant colors that orchids have to offer. After hearing great reviews from friends that visited the exhibition, I knew I couldn’t miss the opportunity to be mesmerized by orchids myself. Roaming through rooms filled with orchids was a magical experience. Your environment is transformed into a tropical paradise of thousands of blooming orchids. Some of them hang from the ceiling and others grow from patches of moss positioned on a man made tree. Every turn of the corner and you will see a unique combination of colors. The variation amongst different species is incredible. One of my favorites reminded me of a sunset, she had the perfect gradient of yellow, peach and pink. I was surprised to find an orchid that happen to match my hair color, which is an aqua mint green. The beauty of orchids will truly take your breath away. The orchids had my undivided attention and I enjoyed spending time to appreciate their beauty. Sometimes we all need an afternoon to look at nothing but pretty flowers 😉 If you missed the orchid show this time, be sure to come back next year for a mesmerizing experience!
This lovely bee is the 610th insect that we collected this summer for the Pollinators on Roadsides project. There's only one container of bees left to pin! This project is funded by @mnenrtf. https://t.co/VgJYZGbdo8
