The lab was buzzing today as we bee-stowed Mike, one of our loyal volunteers, with an official Achene County of Echinacea Empire Passport! This celebration marks Mike’s return from Bee Land, where he has been residing since this past September.
For the past many months, Mike has been working tirelessly on pinning our bee specimens for the Yellow Pan Trap project. This project tracks the changes over 20 years in bee abundance and species composition along roadsides in western Minnesota. You can read more updates on the Yellow Pan Trap project here!
As Achene County’s Passport Specialist, I got to present Mike with his honor.Trying to keep a steady hand as I apply the official visa stamp.
Mike has been working on the samples we collected during the 2022 field season, and just a few weeks ago he finished pinning 789 bees! I can’t bee-lieve how many specimens that is!
The contents of Mike’s passport after the official visa stamping. Please notice the subtle details of the Achene County watermark.
The pinning project required steady hands, an attention to detail and the extensive knowledge of bee identification; all characteristics that Mike has! He spent many hours with the microscope sifting through vials of insects.
From left to right: Mike (elated recipient), me (Lindsey, honored stamper), Wyatt (talented ink pad bearer), and Stuart (head of Achene County, striking a Vanna White-esque pose).
No Achene County celebration is complete without, you guessed it, a sweet treat! The most famous baker in all the land, Alex, crafted a beautiful honey cake drizzled with chocolate and surrounded by pear slices. The ornamental bees on the top of the cake are made up pears (for the body) and dark chocolate shards (for the wings).
I could drone on and on about how good this cake was. It almost made we want to do a waggle dance, but that felt inappropriate given the species of bees we collect.
We are so excited that Mike is no longer a solitary worker bee! We are also excited to send all of Mike’s specimens over to Zach at the University of Minnesota for identification.
The slicing of the cake for all citizens to enjoy. The cake looks particularly similar to our randomizing wheel pictured below Mike’s arm. Coincidence? You tell me.
If you get the chance, please welcome back Mike to the Echinacea hive!
Remember when I talked about only having three batches to work with for the 2022 Liatris data? That is now no longer the case. Thanks to several volunteers continuing to chip away at the remaining Liatris plants in 2022, batch four got officially completed last week. Now with the newly completed batch, I managed to update 2022’s Liatris data in the figure above. It might not be noticeable just by looking at the graph, but the slope and p-values changed quantitatively. The p-value for 2022 now sits at roughly 0.09, which is better than what we had prior (I think it was 0.16 if I remember correctly). However, that is still not as good as 2021’s data, and that data still holds the better slope between the two. Outside of adding the fourth batch, I also tweaked the graphs to be more easily read and understood. I added some color to the data points, reworded the axes, and made the linear regression line bolder for all of you to see better. Overall, these were the updates I have to share with you, but I also wanted to inform you that I have started the process of creating the final poster. Over the course of this week, I will focus primarily on completing the poster, and I hope to have it ready to go by the last week of April. I will be presenting it to you all on April 27th (subject to change), so I hope you look forward to it. It has been quite a journey for this project, but it sure has been an interesting one, to say the least.
During the summer, we use two high-precision Topcon GPS units to map thousands of flowering Echinacea plants. Our GPS units are named Collins and Taylor, after two inspiring female scientists.
Dr. Margaret S. Collins
first Black woman to hold a PhD in entomology
researched defense mechanisms in termites
nicknamed the “Termite Lady”
civil rights activist
Dr. Marie Clark Taylor
first Black woman to hold a PhD in botany
researched photomorphogenesis, how light affects plant development and flowering phenology
developed high school science curriculum now used across the US
her curriculum promoted the use of real plants and microscopes in the classroom
Dr. Collins and Dr. Taylor were friends in real life, just like our GPS units that are often in the field together!
Two large packages arrived on the doorstep of the Chicago Botanic Garden this week containing 40 (yes, 40!) brand new emergence traps! This summer, we are conducting research on the impacts of prescribed fire and fragmented patch size on ground-nesting bees. This new research is funded by ENRTF!
To make sure the emergence traps were functional, Alex and I decided to assemble one in the lab. The assembly was fairly easy, but we noticed that there were some plastic pieces that connected two fiberglass rods that may fall off or get lost easily; sounds like a job for some super glue! We also realized we will need to purchase a heavy object, like a chain, to lay around the base of the emergence trap to prevent it from flying away in the wind!
Looks like we caught someone already!Alex assembling the collection head and bottle, sans killing agent.
Last week, the Chicago Botanic Garden ended their annual orchid show and opened up all their plants for purchase to garden members and employees! Alex and I ventured to the sale at 10 am last Thursday to find a long, looping line that weaved throughout the Regenstein center. We waited about 25 minutes to reach the beginning of the line. We were not prepared for the carnage we were about to witness.
Elbows were flying, emotions were high, plants were abundant. There was a good deal on tiny Phalaenopsis, 2 for $10. At one point, a man tried to purloin Alex’s beautiful orchid while she went to return a shopping basket, and I had to protect the plant from danger. Alex described the ordeal as “a mad house” and “chaos”, and I definitely agree with that assessment.
You can’t tell, but I’m smiling REALLY big because of my orchid purchases.
Nobody tell Jared, but I walked (or, more like ran) out of there with 7 orchids (some to give to family and friends, and one or two for myself). Alex ended up with 3 stunning orchids as well!
The line to check out was slightly calmer, though equally as long as the line to enter the sale. Many people were becoming agitated, having to wait in line and carry their heavy load of orchids. Alex noticed that each cash register had a number (as you can see in the photo below) and each number was a different font! How silly!
Calm after the storm (sort of) while waiting in line to purchase our orchids.
For anyone wanting to brave the orchid sale in future years, my only advice is to prepare for battle.
We have data! So far, we have seen some fascinating results from the 2022 data we worked on over the past 6-7 weeks and the 2021 data collected last year. The data shown above is still in its preliminary stages, and there is still some additional work to be done, but we can make out some things with what we got so far. First, 2022’s data is on the left, and 2021’s is on the right. Comparing the two, we did find that the predation rate, on average, was higher for the 2021 plants than those in 2022. We also created a linear regression line for both years to examine the correlation between headcount and predation rates. 2021’s data had a higher slope than 2022’s, meaning the data in 2021 had a more substantial representation of the predation rate increasing with a higher headcount than in 2022. Overall, these findings suggest that the plants in 2021 saw much more predation activity than those in 2022. With that in mind, it makes me wonder why predation rates are higher in 2021 than in 2022 and what the results would look like if we were to combine the data. This is only the beginning of our data collecting and interpretation, so I’m looking forward to learning more about the results we got and if we can reject the null hypothesis in the end. Note that the null hypothesis states no relationship between headcount and predation rate.
And hast thou pinned the pan trap bees? O frabjous day! Callooh! Callay!
Today, Mike finished pinning all the bees that we collected in summer 2022 for the Pollinators on Roadsides (aka Yellow Pan Trap) project! We started out with seven coolers full of vials, and Mike has been diligently pinning pollinators since late September. In total, he pinned 789 insects! We are very grateful for his help.
The next step is to add informative labels to record collection date and location for each specimen. Then, we’ll send the bees to Zach Portman, the bee taxonomist at the University of Minnesota, for identification. Overall, we collected more bees than I expected based on the last three years. I’m very curious whether there are any differences in species diversity between years. Stay tuned!
year
# bees collected
# traps
# collection days
avg # bees/trap/day
2004
591
20
7
4.2
2017
552
40
7
2.0
2018
430
37
6
1.9
2019
372
38
6
1.6
2022
789
39
7
2.9
Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR). The Trust Fund is a permanent fund constitutionally established by the citizens of Minnesota to assist in the protection, conservation, preservation, and enhancement of the state’s air, water, land, fish, wildlife, and other natural resources.
This semester at school (University of Minnesota Twin Cities), I have been continuing the Dust Project I was working on over the summer. From the work this summer, I had collected 64 heads of Echinacea angustifolia that were then brought down to the Twin Cities for me to continue working on. Just recently, I finished cleaning them (many lovely mornings were spent on the St. Paul Campus, I was lucky enough to have a window in front of the counter I was working at). While it felt a little weird to see the flowers after hiking through a blizzard, it was exciting to see a little bit of the results from this summer’s hard work. There was one head that decided to split down the middle, I found that intriguing. It made me wonder whether something was living in it at one point, or if something else caused it to divide! Currently the heads are being mailed down to CBG to get x-rayed, then I will start the process of counting the full achenes to see if the dust made a difference in its reproductive success.
Today, Drake gave a great talk titled “Investigating the effects of parasitic plants in tallgrass prairies” for the Plant Science Department at the Chicago Botanic Garden. We enjoyed hearing an update on his research and peeking at some preliminary results! According to Lindsey, “The talk was pedicularily riveting and he really comandra’d the room. Drake is producing cuscuting-edge science. His research agalinis with broader conservation and restoration goals in the tallgrass prairie.”
We are so close! We are now a little over two-thirds of the way done with the third batch, and things are starting to get exciting. I want to thank those who helped with randomizing; we would not have gotten this far without your help. With that said, there are now less than 20 plants left to randomize, and we are just about ready to move on to the data entry and interpretation steps. We should have randomization wrapped up by next week, so we should hopefully have our results in by next week. After that, we will then analyze the final results and begin to construct a final poster. More details will be provided for the poster, but in the meantime, we are quite excited about the next steps once randomization is complete.