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Echinacea Project 2024 I’m Hailey, I just graduated from Morris Area High School. I plan on attending Augustana University next fall. I have not yet decided my major. Pronouns: she/her Research Interests I am interested in learning more about the ground nesting bees. It has always interested me and I am excited to see how the bee traps go this season. Statement I have lived in Donnelly, Minnesota my whole life and in my spare time I enjoy reading, running long distance, and paddleboarding.  Members of Team Echinacea are freshly returned from a successful burn outing! We completed five experimental burns during our trip to western MN. A major win for the experimental design of our MN ENRTF funded research on prescribed fire and ground nesting bees as well as for conservation! Here is the scoop: Late Friday night we got to the Hjelm House and were lucky enough to catch a glimpse of the Aurora Borealis. In hindsight, I think this auspicious sighting boded well for our good fortune in burning conditions. On Saturday morning we headed to Torgeson’s to burn the northern unit. Because it was predicted to be a dry day, we got an early start and were ready to burn by 10:45. We got to see lots of spring flora before the burn: pucoons, violets, sedges, pussytoes, etc… These plants will be even happier next year. With help from Lee, the burn went very smoothly. Torgeson’s is a hilly site, so the burn was slope driven and we had a great view. Things are pretty greened up in the area, so the burn was quite smoky. USFWS, who were burning a big restoration right next door must have been burning cattails- look at that thick, brown smoke coming from the other side of the road. Almost like we are having bonfires at neighboring campsites. Cute!  Next we ventured into Grant County. No burning there- and if you’ve been following along that may not be a surprise to you. So it was back to Douglas Co. After lunch the crew headed to hutch to burn the western unit. Another Hilly site. We prepped our breaks and were ready to go! Burn number 2 down! Sunday was a no-burn day because wildfire smoke from Canada was hanging over many counties in MN. A great day to get work done on the porch and venture into Morris to revisit old haunts. We anxiously awaited the next day’s updated report on burn restrictions. Monday morning we got the go ahead to burn in Grant county!! We called in our backups (former team members Daytona and Liam plus a few of Liam’s friends) and headed to Yellow Orchid Hill West to train our first time burners in at a smaller site. The wind was squirrely but our burn was successful and we were just a hop, skip and a jump away from the revered hulze unit. After 3 failed attempts, was this finally our day?  Yes! Drive down highway 55 and you will see for yourself! A hilly expanse of charred earth! A sight for sore eyes after the last burn way, way back in 2003. By this time, restrictions were lifted in Douglas Co. and the crew headed to Nice Island for a final burn before calling it a day and heading back to the farmhouse. Over Jean’s rhubarb cake we envisioned a utopian or maybe dystopian future on team Echinacea where we have a high tech call center and everything is drone operated. We’ll keep you posted on the call center and the rest of the burns we hope to get done this season. There are so many interesting questions to ask within the study design of our MN ENRTF funded research on prescribed fire and ground nesting bees! We are lucky to collaborate with researchers in the area who are taking them on.  Justin Kjorness, undergraduate at Minnesota State University-Mankato, worked with Drs. Mrganka De and Matt Kaproth (and many more) to collect soil samples at our remnants and restorations this summer. He has since been asking questions about the effects of prescribed fire regimes on soil physical properties. He presented these results at the MSU-Mankato research symposium this week! Learn more about Justin’s results below! Ants are an integral part of ecosystems, playing a role in seed dispersal, detritus removal, pest predation, and nutrient cycling. Because ants nest in the ground, they are particularly susceptible to any process that disturbs the earth and can be heavily impacted by land use practices and management decisions. Diane Roeder, at Augustana University, designed this survey to quantify ant species diversity in remnant and restored prairie patches in western MN. These sites are primarily managed by fire, a type of disturbance that has been hypothesized to impact ant species differently via mortality and/or changes in habitat structure. During the summer, Diane and members of team Echinacea sampled 45 prairie sites (30 remnant, 15 restored), deploying a total of 415 pitfall traps. Diane and her colleagues are in the process of sorting ants from other ground-dwelling invertebrates captured by the traps and will identify specimens to compare abundance, species richness, and community composition from sites under different management regimes. In addition to measuring diversity, They also deployed sentinel prey items to determine whether arthropod communities in these areas remove prey at different rates as a measure of ecosystem services provided by predatory arthropods. To do this, they set out small cages containing moth eggs and recorded the number of eggs removed. In the future, Diane hopes to compare the overall arthropod communities between these types of sites from multiple years of sampling.  
For the ENRTF-funded research project investigating fire effects on ground-nesting bees, plant-pollinator To obtain robust inferences, it is important to sample randomly so that our sampling effort is not biased by what we perceive to be “good” or “bad” habitat, even subconsciously. To this end, we sampled at random locations within each site. At each site, we established between 30 and 72 sampling locations with unique identifiers (four-digit bbpts, for “burning and bees sampling points”). Early in the summer, before sampling at these points began, we ground truthed the points to ensure we were not choosing in places where we could not sample at all (e.g., think a big rock, a water body, a big patch of poison ivy, a gravel road, etc.). Jared generated a large number of random points for each site, more points than we actually intended to sample. trap. We visited these points using a high precision gps unit and evaluated whether to “keep” the points and assign them a bbpt or “reject” the point if it could not be sampled safely or effectively. 
During summer 2023, Team Echinacea Echinacea characterized local environmental conditions to better understand which environmental factors are associated with good habitat for ground-nesting bees. This microhabitat assessment complemented emergence trapping for our ENRTF funded research on fire’s influence on ground nesting bees habitats. We sampled local environmental conditions near randomly placed bbpts in remnants and restorations. We used a light meter to quantify light availability via a measure of photosynthetically active radiation. We took PAR readings at 1 meter and at ground level ~40 cm NE of the bb point. We also used a soil penetrometer to quantify soil compaction at ~40 cm NE of the bb point. Team Echinacea conducted microhabitat assessments for three rotations of bb points (rotations 1,2,&3) across 46 sites. Over the summer, we took microhabitat assessment measurements at a total of 1,238 bb points. 
During the summer of 2023, Team Echinacea conducted floral surveys at randomly selected bb points in remnant prairies and restorations. We are interested in quantifying floral resources (i.e., food for bees) and we want to understand how fire influences the diversity and abundance of flowering plants. At each focal point (bbpt) we identified species rooted within a 2 meter radius and recorded the furthest stage of development. We measured abundance by binning a range of floral units (i.e., 1-5 flowering units got label “5”). Floral surveys were split into “visit group A” and “visit group B”. We surveyed different random points when revisiting sites. In total, we conducted 415 floral surveys across 45 sites. Liam Poitra, a 2023 Summer Research Experience for Undergraduates (REU) Participant, contributed to this research project investigating the effects of fire on diversity and abundance of flowering plants. Liam assisted in fieldwork and data organization. Inventory, protocols, and blank datasheets for floral assessments are located in ~/Dropbox/enrtf/floralSurveys2023. 
In 2023, Team Echinacea did not conduct any fieldwork for this experiment. Pollinator populations are declining worldwide, and pollinator habitat in western Minnesota has diminished over the years, but it is unclear whether the native bee community is changing as well. The Pollinators on Roadsides project, also known as the Yellow Pan Trap (YPT) study, is investigating how native bee diversity and abundance have changed from 2004-2022 and learning about whether the amount of agricultural land and grassland correspond to the nearby bee community. In the lab, rock star pinner and volunteer Mike Humphrey finished pinning all 789 bees from 2022 on 6 April, 2023. Intern Alex Carroll brought the bees to Zach Portman, the bee taxonomist at the University of Minnesota, for identification on 6 June, 2023. Zach recently reported that he’s all done with our 2022 bees, and we will be picking them up from UMN next chance we get!  Alex worked to put datasets together (view in Dropbox/ypt2004in2017/yptDatasets/) for this experiment to prepare us for when Zach finishes his identifications. Alex also created this to-do list of next steps:
Summary
Funding for this project was provided by the Minnesota Environment and Natural Resources You can read more information about the pollinators on roadsides project here. In July, 2023, a team from Minnesota State University, Mankato visited Team Echinacea to sample prairie soil. They are investigating how fire and management history influences physical, chemical and biological soil properties. Differences in soils may also help explain plant fitness and native bee nesting patterns. After three hot days, 263 samples were collected from 28 sites! 
In 2022, Team Echinacea collected an additional summer of data for the Pollinators on Roadsides project after receiving funding through the Minnesota Environment and Natural Resources Trust Fund (ENRTF). Pollinator populations are declining worldwide, and pollinator habitat in western Minnesota has diminished over the years, but it is unclear whether the native bee community is changing as well. The Pollinators on Roadsides project, also known as the Yellow Pan Trap (YPT) study, is monitoring how native bee diversity and abundance have changed from 2004-2022 and investigating whether the amount of agricultural land and grassland correspond to the nearby bee community. In summer 2022, Team Echinacea installed pan traps at 39 of the 40 locations that were used in previous years. The traps were placed along roadsides in Solem Township, and Geena Zebrasky also surveyed the plant diversity surrounding each trap. Geena and Alex set out the traps 7 times during the summer, once per week starting on July 7th and ending on August 17th. We filled the yellow bowls with soapy water each morning starting at ~8:00 am and collected bees from the traps in the afternoon starting at ~4:00 pm. We strained the bees through a sieve and stored them in vials filled with 70% ethanol. Over the summer, we collected seven coolers full of vials, which are now stored in the freezer in Illinois.     At the lab, volunteer Mike Humphrey has been pinning the bees that we collected during the summer. So far, Mike has emptied three of the seven coolers, and he has pinned 420 specimens from 90 vials. Each specimen is assigned a specimen id number (SPID), which started with SPID 20001 this year. In previous years, we glued tiny bees to point mounts, but this year we are gluing them directly to a #2 pin based on a recommendation by Zach Portman, the bee taxonomist at the University of Minnesota. Last winter, we sent Zach the insects that had been collected in 2004, 2017, 2018, and 2019, and he identified the bees to species. Over the 4 years, we caught 1,901 bees from 76 different species! The next steps are to finish pinning the bees from summer 2022, add descriptive specimen labels, and send the bees to Zach for identification. Mia and Alex are working on a bee community analysis and a landscape analysis.  
Funding for this project was provided by the Minnesota Environment and Natural Resources You can read more information about the pollinators on roadsides project here. |
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