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Dayvis Blasini Research Proposal

Echinacea Project Research ProposalPDF.docx.pdf

Marie’s Research Proposal

Hello all,

This is the first draft of my research proposal regarding fitness and heritability in the offspring from Shona’s crosses last summer. I still have a bit more research to complete – in particular, brushing up on quantitative genetics. Nevertheless, I have enough information to go forward, and hope to get a good chunk of my measurements done by next week.

Schaedel_ResearchProposal.pdf

-Marie

Another Thursday on the beautiful prairie

Today the team accomplished a variety of projects. The morning began by searching for grasses in the common garden. A decent amount of grasses were located and the garden was resounding with choruses of “woots” shouted out when the grasses were located. The rest of the morning the team worked on individual projects. Throughout the day, Pam and I measured the Amax, transpiration, and conductance of echinacea plant leaves in the hybrid garden within the common garden. We managed to measure 42 plants before Helga (our fabulous machine) needed to take a rest and recharge until tomorrow. In the afternoon, more grasses searches were done. The team also ventured out to Hegg Lake to help Kory find echinacea plants about to flower in common garden 2 and to help Davis find flowering echinacea pallida plants. Overall the day was beautiful to be outside, and it was a very productive day! -Reina

Helga

Remnant Flowering Phenology and Seed Set Analysis

Hi there everyone,

I was an intern with the Echinacea Project last summer (2012) and worked on the remnant flowering phenology project. Over my winter term at Carleton I worked on an independent study to analyze the seed set of some of the remnant plants. After finishing dissection and x-raying the achenes (with a lot of help from the lovely volunteers in Chicago!), I created a poster which I presented at the Midwest Ecology and Evolution Conference in March of this year. I have attached the poster to this post. Hopefully, it explains some cool findings!

Good luck with field work everybody!
kapsar_MEECposter.pdf

A brief intro- Kory Kolis

Hello everyone! I am Kory Kolis and am from Eau Claire Wisconsin. I am a Junior at Gustavus Adolphus College in St. Peter Minnesota (about an hour southwest of the twin cities). I am a biology and studio art double major. Last summer I was doing biochemistry research at Gustavus examining the four proteins that make up the kinetochore of Saccharomyces cerevisiae. I am very excited to be a part of Team Echinacea this summer, and for the opportunity to work outside! Woot!

This summer I plan on continuing the research done by Katie Koch and Andrew Kaul. I will be looking at the efficiency of pollinators on the Echinacea angustifolia. By doing my observations in Jennifer Ison’s common garden I hope to be able to be able to trace the pollen back to the parent plant, allowing me to see if there are any relationships between distance from the two plants and pollinator.

In my free time I love making art. My favorite medium is ceramics, but above all love making sculptures. I recently put on a small show of my artwork in my school library titled “The Alchemist.”

If you would like to see the link of me on the Echinacea Project home page click here.

Team work in week two

The second week of the 2013 field season for Team Echinacea was excellent. We finished searching for seedlings and found a grand total of 102 seedlings in 13 remnant populations. We laid out the main common garden experiment with over a thousand orange, blue, and lime flags to guide our walking and to enable us to identify individual plants. We also began assessing survival in the recruitment experiment. On Wednesday Ilse presented results to the team on her aster analysis of 17-year fitness records for about 600 Echinacea plants in our main common garden experiment–details to follow. Pam took out her big new photosynthesis machine for its first trial run. Storms and wetness rained us out all day Thursday and we were without power for two hours on Thursday and about 18 hours on Friday. Team members are refining their ideas for independent projects and soon will be able to make their own posts. (IT folks at the UMN said they fixed the access problems-we’ll see.) Stay tuned to read about their awesome experimental plans!

You can read about some of our team-members on our their Echinacea Project webpages…
Pamela Kittelson
Ilse Renner
Dayvis Blasini
Kory Kolis
Sarah Baker
Marie Schaedel
Reina Nielsen
What a great team!

Next week we aim to finish assessing survival, flag another experimental plot, measure more plants, work on independent projects, and purchase/make/organize equipment and supplies for our experiments. We are also looking forward to Amy Dykstra’s visit. She will talk about her dissertation research.

We are making updates via twitter and facebook. These media have proved to be more reliable than this flog, but we hope that changes soon. See links on the Echinacea Project’s main web page. We hope to set up a venue for sharing more of our photos–stay tuned for that, but here are a few photos from this past week…

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Searching for seedlings at LF (the landfill site).

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An orchid (Cypripedium calceolus)
flowering at the KJ site.

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The team on the porch of the Hjelm house.

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First trial of the new phtosynthesis machine.

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Sorting flags to reuse & recycle.
We estimated 2600 flags here to reuse.

More defending your thesis

I defended my thesis on May 16th, presenting the results of my research on the hygroscopic motion of big bluestem and indian grass. I’ve attached the presentation to this post, though the presentation is a bit light on text. I’m putting together a section on my website with more text, which I’ll link when it’s ready.

drizin-seeds-presentation.pdf

Field trip to MN (22 – 26 May)

I drove from the Chicago Botanic Garden to our field site in western Minnesota hoping for a window of appropriate burning weather on Thursday afternoon or Friday afternoon. I also brought 297 Echinacea seedlings to plant as part of an experiment that investigates hybridization between native and non-native Echinacea. Several gallons of side-oats grama grass seed were waiting to be hand broadcast at two sites after the burn.

Why burn?
We want to burn our large Echinacea “common garden experiment.” In this abandoned field we have planted about 14000 individual Echinacea plants, starting in 1996, and measured their growth and flowering every year. We have burned this ~6 acre plot every other spring from 1998 to 2008. The weather didn’t cooperate in 2010, so we burned in 2011. We are trying to burn this year! Burning in the spring really increases the chance that an Echinacea plant will flower. We are planning a big crossing experiment this summer, so we want as many plants to flower as possible. Also, burning sets back the weeds–and that is a good thing.

Here’s the quick recap of major activities.
1. Packing
2. Driving to MN
3. Preparing to burn
4. The burn
5. Seeding after the burn
6. Preparing to plant
7. Planting
8. Seeding the phenology plot
9. Driving to IL

photo(3)May2013.JPG

Dwight makes sure the fire stays where it belongs

Read the gory details…

1. Packing
I loaded the truck with lots of field equipment to use during the summer. The seedlings in the hybridization experiment fit in three trays. I had two trays of “extra” seedlings that were still hanging on from Jill’s agar/blotter seed germination experiment. For the burn, I brought three backpack sprayers, the DNR burn permit, and batteries for radios. I forgot to bring a back-up drip torch.

2. Driving to MN
The drive to Minneapolis was generally uneventful, which is just what one wants when driving an 18-year old truck. Very early on Thursday I drove to the site. Shortly before arriving, I saw two Sandhill Cranes landing in a wet field near Holmes City. I think that’s the first time I’ve seen cranes on the ground in Douglas County–auspicious! I should mention that I also saw a Common Loon and Pelicans, which are common every summer in Douglas County. Seeing them makes for an auspicious day too.

3. Preparing to burn
Thursday morning the ground was wet and the wet grass duff (fuel) didn’t look very promising for burning. The erosion in the surrounding corn fields indicated it had rained a lot. Our rain gauge confirmed that showing 3.65 inches (93 mm) of precipitation in the previous week. Light winds and cool temps weren’t helping dry it out, but the sun and low humidity offered a glimmer of hope. It’s rather stressful trying to predict burn weather and decide whether to rally folks to make a long trip to help burn.

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The plot before the burn

Dwight and I began preparations for the burn. Just after noon, it looked like the fuel might dry out enough to burn and the weather forecast looked great, so I let folks know that we were going to attempt to burn in late afternoon. I should add that the fire forecast for Friday was getting worse, so that a burn was going to happen on Thursday or never (well, June or 2014).

Here’s our pre-burn to do list:
Prepare burn break (mower, weed whip, chain saw)
Fill water tank on truck (70 – 80 gallons)
Put six buckets in tractor trailer
Set up hoses [I forgot to set up hoses this year, thus this list]
Fill backpack sprayers
Fill drip torch(es)
Get matches
Activate burn permit
Call sheriff
Call neighbors
Walk burn break with crew & place buckets
Check radios

What to wear during a burn:
100% cotton long-sleeved shirt and long pants
Hat
Leather boots (over ankle, if you have them)
Leather work gloves

4. The burn
Amy, Brad, Dwight, Jean, and I conducted the burn and it went smoothly. Amy made a nice post with photos. The burn was slow and thorough. Ignition initiated at 5:20 and we were done at 8:20. Winds were light ESE ~ 8 mph. We had a great dinner afterward.

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Amy keeps the fire from jumping the road

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Brad and Amy through the smoke

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A slow and thorough burn

Post burn to do list:
Call sheriff
Put everything away–dry out tanks
Broadcast seed

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Some grass is still green right after the fire

5. Seeding after the burn
On Friday I hand broadcast about 2 gallons (8 liters) of native grass seed. More warm season grasses will provide fuel for future fires! The Soras chattering in the neighboring wetland kept me company. Here’s the seed sources–keeping it local!
grocery bag: Bouteloua curtipendula from cg1 10 Aug 2012
grocery bag: Bouteloua curtipendula from Krus 14 Aug 2012
#1 bag: Bouteloua curtipendula (& some Elymus canadensis) from Backhill 19 Aug 2012
#2 bag: Bouteloua curtipendula from Backhill 19 Aug 2012
#2 bag: Bouteloua curtipendula from cg1 22 Aug 2012

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Much of the grass that was green right after the fire is now brown

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I found this Sharpie while broadcasting side-oats grama


6. Preparing to plant

Next job was getting those seedlings in the ground! The forecast was for lots of rain during the upcoming week, which is great for seedlings, and for lots of rain in the afternoon, which is not so great for a person planting seedlings. I had to hurry.

I was quite efficient, except that I paused to watch a pair of Trumpeter Swans at Hegg Lake WMA–another first for me in Douglas County. I got a very nice look at the birds up close and saw the shape and color of their bills–straight and all black. I didn’t hear them, so I’m not 100% positive they were trumpeters. Tundra swans apparently look similar, but I haven’t seen them up close for years.

I chose a uniform location in an area that was planted with warm season grasses in 2000 for this experimental plot. I was considering mowing the area, or at least the rows to facilitate making straight rows and putting the plugs in, but I decided against it.
I laid out 300 planting locations (10 m x 30 m) with meter tapes and pin flags. A 9m x 29m grid would be sufficient for 300 locations, but I don’t like to plant at 0m on a tape and I planned to leave a few flags at locations with no plants.

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Setting up planting locations

7. Planting

These seedlings all originate from plants that flowered at Hegg Lake last summer. Shona conducted the summer experiment and prepared the seeds for germination. Read about Shona’s project. Jill germinated the seeds this spring and measured the seedlings multiple times. Read about Jill’s project.

I ended up planting one tray (~100 seedlings) on Friday before getting rained out. Planting in the light rain worked for a while, but it was slow. When it really started coming down, I bagged it.

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tools of the trade

On Saturday morning I saw the pair of Trumpeters at the flooded field SW of MN27 & CR1. The nearby Canada Geese were much smaller. A black tern flew overhead. When I arrived at Hegg, I saw fresh truck tracks and what looked like a can of beer and a pair of underwear right in the middle of the parking area. They were not there the day before! Upon closer inspection I realized that it was a can of Nos energy drink, not beer.

Saturday proved to be cold but not too cold. It was 54 degrees F and very windy. I wore nitrile gloves and that kept my hands warm. I planted almost twice as fast as I did when it was raining. The Bobolinks kept me entertained all morning with their bubbling songs.

8. Seeding the phenology plot Saturday 1:11 – 1:42 pm
Right after finishing planting I went to the phenology plot and broadcast some native seeds that we collected last fall. I broadcast the seed fast and it dispersed well in the strong and gusty wind. There is a new fox den with a big pile of dirt in the middle of the plot. The only plants I saw flowering were Sambucus, Dandelion, and Antennaria. I’m sure others have been flowering, but it was overcast and cold and I was moving fast. As I left Hegg Lake a wild Turkey walked out in the road in front of my truck. So nice to see.

I planted ~ 2.5 gallons of seed from three collections:
1. Bouteloua curtipendula from Hegg Lake WMA 13 Aug 2012 (#4 bag). 2. Bouteloua curtipendula from NE corner of Hegg Lake WMA 27 Aug 2012 (grocery bag). 3. Schizachyrium scoparium (& some Bouteloua curtipendula) from along CR 15 at Hegg Lake WMA 16 Sept 2012 (grocery bag).

9. Driving to IL
I left around 5:45 AM on Sunday. As I climbed in the truck I heard a Great-crested flycatcher–first of the season–so, I suspected it would be an auspicious trip. It was a boring drive, just the way I like it. I talked to myself about mating isolation and asynchrony to help prepare for writing a proposal to NSF on this topic.


Postscript.
I didn’t have time to plant a few of Maria’s left-over Dichanthelium plants. Jean said she would be glad to plant them in her prairie garden. photo(7)May2013.JPG

Maria’s Dichanthelium plants. Some have been exposed to cold temperatures for the past month or so.

until next year

There was one project we did not get to this year. In the summer of 2012, field intern Kelly Kapsar observed flowering times for Echinacea in burned and unburned areas of the Staffanson prairie preserve. At the end of the summer, she collected the flowering heads in order to assess the relationship between flowering time and seed set. Various students have worked on aspects of the project. In November, Mindy Runge and Ale Mendoza (Lakeforest College) extracted and weighed achenes (click here to see their poster). In December, Marie Schaedel (Carleton College) weighed additional achenes and assessed our methods of judging whether an achene is full or empty (click here to read her results ). Most recently, Jill Pastick (Lakeforest College) finished extracting achenes and helped organize the collection.

These steps (extracting and weighing achenes) allow us to draw a relationship between flowering time and seed set (i.e. the proportion of full vs. empty achenes). The next step of this study will be to germinate the achenes and plant them in the field. Unfortunately, that will have to wait until next year. In the meantime, the collection of weighed and organized achenes will be stored in the freezer.

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Maria’s Poster for MEEC 2013!

Hi everyone,

I presented a poster at MEEC 2013 (which Katherine wrote on in the previous entry) and just got back from another poster presentation at Chicago Area Undergraduate Research Symposium (CAURS) today!

Here’s my poster – enjoy looking at it to see what I found out from my summer fieldwork!
Wang_MEEC2013-Poster36x44-pdf.pdf