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The seedling search has begun!

Today we started the seedling search at Steven’s Approach (SAP). The wind was strong and air temperature was chilly. We searched 3 circles; in one of the circles we found 6 seedlings! We drew a map and filled in a matrix, as we have done in previous years. We also tried out the new coordinate frame.
I (Amy) have revised the protocol. Please read it and feel free to suggest ways it can be improved.
Seedling Search Protocol 2011.doc

Stipa and the common garden

I’ve been working with the Stipa germination data we collected from the common garden over the summer for Stuart’s R class and, among other things, have come up with a little plot of the common garden. Filled-in blue circles are where we found Stipa alive, empty circles had no seedlings. A neat thing would be some kind of heat map for longest leaf or number of leaves, but I’ll try that later.

View image

Fall Field Work

Kate and I have had some beautiful weather for field work (except today–it rained most of the day). Here’s a picture of the roadside site we call East Riley. Notice that our ambitious mower has mowed TWO swaths of the roadside. The mowed area includes most of the circles I am searching to re-find and measure plants we had identified in years past.
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We do seedling searches every spring at 14 prairie remnants. We search within 41 cm (or 50 cm for smaller populations) of randomly selected Echinacea plants that flowered the previous summer. Since 2006 we have used these spring searches to find new seedlings (identifiable by the presence of cotyledons). When we find seedlings, we draw circle maps showing the seedling locations with respect to the focal plant, and make measurements to other tagged plants. In 2009 and 2010 we mapped ALL Echinacea plants within the 41/50-cm radius circles. Late in the growing season, we return to the 14 sites and re-find the seedlings and other plants. We update the circle maps, and measure the surviving plants.

Why go to all this trouble? I plan to use the data to estimate the growth rate for these small Echinacea populations. Are the Echinacea producing enough offspring to maintain their populations? That’s the question I hope to answer!

The re-finds are complete at eelr, lih, nessman, nwlf, randt, sap, sgc and spp. I am currently working at eri; still remaining are eth, kj’s, lc, lf and ri. Some of the sites are disappointing. There has been a lot of gopher activity at lih, and most of the Echinacea plants, big and small, are gone. I was only able to find 1 of the 12 seedlings we had previously mapped. Other sites (eri, ri, nessman) are disturbed by frequent roadside mowing and scraping. In spite of that, we were able to find 18 of the 23 previously identified seedlings at nessman. In total (so far), we have found 54/85 seedlings. Some of the survivors were first identified in 2007 (I don’t think I’ve found any 2006 seedlings yet–but there weren’t many to begin with).

I’ll post an update when I finish entering all the data…or the next time I get rained out!

end of harvest

Only about 13 heads left to harvest in the common garden experiment! I attached a pdf of listing all heads with no gbag info…

HarvestList2010FinalHarvest.pdf

Here’s a map of unharvested head locations in the main experiment (there’s one more head in the 99S garden).

toHarvest.png
Click to enlarge.

Seedling refind protocol 2010

Here’s the protocol for re-finds in the remnants. Please look it over, and critique!
Protocol for seedling refinds 2010.docx

Stipa tag found

Tag #1028 was just found in the ground near the SW corner of Hjelm house porch.

Draft 2010 Stipa protocols

2010 Stipa Draft Protocol.pdf. The Google Docs wasn’t really working out… Comment with your thoughts and changes.

3 States in 2 Weeks

Hey All,

It’s been a very busy couple of weeks:

On July 27th I spoke at the Garden Club of America Zone 11 Annual Meeting in Lake Geneva. I was there for less than 48hrs, but I still had a wonderful time. I received the GCA’s Fellowship in Ecological Restoration to support my Master’s project, which is why I was asked to attend at all. I was pretty nervous about the speech, but in the end I think it went pretty well. They were a great audience and after this experience I feel much more confident about public speaking.

After I gave my talk, I was able go see the GCA flower show, which was really interesting. There were some pretty cool displays. I especially like the hats made of flowers, the miniatures, and the photography. They also had these challenge events where everyone was given the same supplies and theme and had a fixed amount of time to create a display. The GCA has some very creative members.

That evening we were given a tour of the lake on a boat called the Louise, where we had h’ors d’oeuvres and cocktails. The boat dropped us off at a GCA member’s house for dinner. It was a lot of fun!

The next day I got to hear Kathryn Kennedy, the president of the Center for Plant Conservation, speak. She gave an excellent talk about endangered species and the challenges we face in protecting these high-risk plants in the coming decades. I was totally revved afterwords, so she did her job.

Pictures from the GCA trip:
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After I got back from Wisconsin, I had to turn around and finish working on the poster for the ESA conference. After much work, and help from Stuart, Amy, and Josh, I finally finished the poster.

I drove to St. Cloud to print both the FNC poster and Allegra’s poster, and then on Sunday Stuart and I went to the ESA conference in Pittsburgh. Mimi, the REU intern from last year, was able to join us for the conference on Monday. It was great to see her again. She’s currently working at Frick park as a Outdoorsy type summer camp counselor. She’ll be leaving in September to teach English in Guadalupe. Should be fun!

Mimi and I got a lot of great feedback on our poster during the poster session, and I’m pretty confident that with everyone’s help we can write an interesting paper.

The poster is entitled: Interspecific Co-flowering Prairie Plants Compete for Pollinators.

Here’s the abstract we submitted:

Background/Question/Methods:

Pollen limitation is prevalent in many species, and can be especially worrisome in fragmented landscapes. Reproduction in the purple coneflower, Echinacea angustifolia (Asteraceae), which grows in small remnant patches of tallgrass prairie in North America, is pollen limited, but not pollinator limited.

Pollen limitation of Echinacea increases with isolation of individual plants, decreases with size of population, and has a strong negative impact on reproduction. However, pollinator visitation does not explain the reduced reproductive success. Wagenius and Lyon (2010), found that plants in the densest and largest populations of Echinacea receive fewer pollinator visits yet have greater reproductive success than plants in small isolated populations, which receive more visits but have decreased reproductive success. Among the many prairie plants pollinated by native solitary generalist bees, interspecific co-flowering plants may either enhance pollination by attracting more pollinators, or reduce reproductive success through competition or interference with compatible pollen receipt (Feldman 2008, Mitchell et al 2009).

Results/Conclusions

We investigated the community of co-flowering species surrounding a plant (floral neighborhood). We measured the effect of floral neighborhood on pollinator visitation, pollinator diversity, and pollination success of Echinacea. We randomly selected 8 focal plants at each of 10 remnant prairie sites in Douglas Co., Minnesota and observed and collected insect pollinators four times during summer 2009. We also identified and counted inflorescences of nearby co-flowering species.

We found strong evidence that floral neighborhood composition influences pollinator visitation of Echinacea. Forty species co-flowered with Echinacea, nine of which are invasive. Co-flowering species richness ranged from 14 in the largest remnant to five in the smallest. Alfalfa, Medicago sativa (Fabaceae), the most abundant exotic co-flowering species, occurred at seven of the sites, while leadplant, Amorpha canescens (Fabaceae), the most abundant native species (besides Echinacea), was only found at three remnants. The presence of alfalfa within a focal plant’s floral neighborhood increased the probability of a pollinator visit by 7% (according to a glm with binomial response p<0.03). In contrast, native leadplant decreased pollinator visits by 9% (p<0.02). There is no evidence that alfalfa, leadplant, and Echinacea interact in their effect on pollinator visits. We also collected pollen from Echinacea insect pollinators and flower parts to see if patterns in floral neighborhood composition and pollinator visitation are reflected in pollen loads on pollinators or stigmas.

Here’s a small copy of the actual poster:
Gallagher_ESA_Poster_Final_sm.pdf

The main conclusions were:

Neither the community of co-flowering species nor the presence of non-native plants was associated with variation in pollinator visitation.

Co-flowering species diversity was a predictor of pollinator visitation only late in the flowering season. Floral communities, as quantified by NMS analysis, were associated with the presence of non-natives all season long and overall diversity only in the early season. NMS characterizations of species co-flowering with Echinacea did not predict pollinator visitation at any time.

Echinacea neighborhoods with Amorpha, a native, had lower pollinator visitation, while the neighborhoods with Medicago, a non-native, had higher pollinator visitation.
We found no compelling evidence that interspecific co-flowering species influence pollinator visitation to Echinacea in small prairie remnants. The previously observed high pollinator visitation and concurrent pollen limitation might result from low quality of pollinator visits, an hypothesis we are now investigating.

Here’s a picture of Mimi and I in front of the poster at the conference:
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Now that I’m back in Kensington, it’s time to get back to my master’s project. The work is never done! This week I’m hoping to do seedling checks for my three plots. Hope I can count on lots of help getting it done!

-Kate

Stipa sticking spree!

Having stickered and scanned all of the collected Stipa seed (great job Ian, Hillary, and Lauren!) we’ve gone through and stuck them all into their foamy rows. 1700ish seeds later, we’re ready to plant out in the common garden! Here’s a few pictures:

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Poster and Powerpoint Presentation!

Hey everyone-

Thanks to all of you who gave me such great input for my poster and/or the powerpoint!

I attached a PDF of the finished poster as well as my slides for the powerpoint presentation because I know I was freaking out about fitting everything in 5 slides! So you can see how it turned out, and I have 4 extra slides if I get questions. haha.

KochkREU10.pdf
power point presentation.pptx

Now I need to practice, practice, practice the presentation! I’ll post one more flog update for how it goes on Friday. Eeek!

-Katie