Reproduction in plants can be limited by access to pollen and resources. We previously found that Echinacea plants in the remnants are pollen limited, meaning that if they had access to more pollen, they would produce more seeds. However, the long-term effects of pollen limitation are unknown. Do plants that are super pollen saturated and have high amounts of pollen have a higher lifetime fitness than plants that are pollen limited? Also, we know that the plants in the remnants are pollen limited, but are the plants in the common garden environment also pollen limited? To answer these questions and more, 13 years ago Gretel randomly selected 39 plants from p1; half of these plants were randomly assigned to the pollen addition group, and the others were assigned to pollen exclusion. Every year, plants in the pollen exclusion have their heads bagged and they are not pollinated, while we hand cross every style in the pollen addition group. An additional 53 plants in p1 were selected to be part of a control group where pollen was neither added nor excluded.
In the summer of 2024, NONE of the original 39 addition/exclusion plants were flowering. If any had been flowering, the exclusion treatment plants would be covered with exclusion bags to prevent pollination, and the addition plants would be hand-pollinated multiple times throughout the summer. One plant in the control group produced a single flowering head.
Collaborator and NDSU postdoc Grace Hirzel places pollinator exclusions bags on Echinacea heads in the remnants. If any of the plants in the polLim experiment from the non-control group had flowered this year, it would’ve looked like this!
You can find more information about the pollen addition and exclusion experiment and links to previous flog posts regarding this experiment at the background page for the experiment.
It has been a packed few days here in Phoenix, and I’d like to describe some of the presentations I saw at yesterday’s ground nesting bee symposium. There was a broad range of topics, including the microbe communities found on pollen balls, brood parasite and host interactions, rare desert bees, and more! Nick Dorian had great results to share from his mark-recapture work in New Jersey, which was great to see after he demonstrated that method to Team Echinacea over the summer. Lily Fulton’s presentation really stood out to me: She is also studying prescribed fire, at a long term experimental fire site in Florida longleaf pine flatwoods. they found higher bee and wasp abundances during burn years, as well as higher proportions of bare ground. This is strikingly similar to our research findings! It was great to chat with her about it over my own poster.
Title slide of Lily Fulton’s presentation. My poster! It is showing the first year of data from the ENRTF project, since we haven’t sent our 2024 bees to Zach yet.
It’s been incredible getting to meet so many researchers who care about native bees. Many stopped by to have a look at the poster! I’ve cited many of them in my previous work, so running into them in person is almost like meeting a celebrity. One consistent interest I’ve gotten from attendees is to look a the community composition of bees in the different treatment groups. Perhaps the increased abundance in the year of a burn we’re seeing is because of a cohort of burn-loving ground nesters, who don’t stick around in following years. It’ll be exciting to look into!
Shot of the poster sessions. There were a ton of applications, so space was pretty limited.
Overall, this has been a fantastic experience and opportunity to expand my network. I got to reconnect with some old friends, and make plenty of new ones. I can’t wait to get back to work on my thesis, now that I know who might be viewing it once it’s published! Expect more on that in the next few months.
As I’m preparing to write updates on experiments for 2024, it was brought to my attention that we don’t have one post summarizing all our updates from summer 2023! So, while you eagerly await this year’s news, enjoy a refreshing blast from the past. Here’s what we did last summer!
Members of Team Echinacea 2023 measure plants in our hybrid experimental plots at Hegg Lake WMA
We know that prescribed fire is beneficial to native prairie plant species, but summer 2023 REU participant, Jak Davis, is also curious about how fires impact native pollinators. Their project this summer, investigated the effects of prescribed fire on pollinator fidelity/visitation to Echinacea. Jak collected bees in the field (Agopostemen virescens, Halictus and Augochlorella) and scraped pollen off their bodies. They counted and identified pollen grains under the microscope and calculated total proportion of Echinacea pollen grains.
Jak is continuing this work at part of their senior thesis! She has started data analysis and will have results to share soon!
Jak has been hard at work in the lab at College of Wooster post-field season!
A closer look at pollen grains through the microscope! Jak is able to tell which grains are Echinacea- impressive!
Start year: 2023
Location: Remnant prairies in Solem township, MN and the lab at College of Wooster
Overlaps with: other projects in prairie remnants
Data collected: N/A
Samples or specimens collected: pollen scrapes from bees, floral specimens
The Leadplant Flower Moth (Schinia lucens), is a species of special concern in Minnesota. It used to be common and have an extensive range, but now populations are few and far between. Douglas and Grant counties (our study area) are not included on its current range map. Nonetheless, REU participant Liam Poitra thought that it was possible that this moth might persist in some of the remnants in our study area. Liam planned and conducted a systematic search for the leadplant flower moth for his REU project. If Liam found enough moths to estimate populations sizes or density, then he would investigate characteristics of their habitat for his REU project. If not, then he would transition to another project. He found one individual moth and took some great photos. Details of the search are in Liam’s report.
Liam marches through Staffanson Prairie Preserve. This site was included in his systematic search for the leadplant moth.
Start year: 2023
Location: prairie remnants in Solem & Land Townships
2023 RET participant, Victoria Romero, spent time over the summer designing a lesson plan for using the ABT format. She implemented this in her project-based learning class. Students presented their enzyme research in a “poster conference” style format. Each student had 3 minutes to describe their enzyme models using the ABT format. This project was implemented early in the year so there was hesitation, but Victoria may plan more ABT format presentations throughout the year.
Victoria also plans to implement ecology focused lessons later in the year. She is teaching an English for second language ecology course, so she may modify her lessons to fit that.
Victoria takes GPS points in the field! Team Echinacea had lots of time to think about and discuss ABTs for our work this summer.
Start year: 2023
Location: Western Minnesota and Gwinnet County Public Schools
Overlaps with: NA
Data collected: NA
Samples or specimens collected: NA
Products: Victoria’s website to introduce her students to the ABT format project can be found here: Enzyme Project Her presentation to the 2023 summer team is located in “Dropbox/teamEchinacea2023/victoriaRomero”
Hemiparasitic plants are associated with higher quality prairies, and many hypothesize that they are fundamental in generating this high-quality prairie potentially by impeding the growth of dominant grasses, allowing non-dominant forb species to establish. Additionally, if this is true, there are likely different effects associated with the abundance of hemiparasites. Therefore, we experimentally introduced Pedicularis canadensis to another restoration plot, experimental plot 10; however, this time we introduced P. canadensis at different densities around 8 different focal species. We measure the size and reproductive effort of these 8 focal species around each of our 66 hemiparasitic plant planting locations.
In 2023, we replanted 32 Pedicularis canadensis that hadn’t been seen since they were initially planted in experimental plot 10. Additionally, we took our annual measurements of our 8 focal species’ size and reproductive effort.
Pedicularis canadensis in experimental plot 10 at WCA high school. Drake planted this hemiparasite in different densities across the experimental plot.
Start year: 2019
Location: experimental plot 10
Overlaps with: parasitic plants addition experiment in p01
Data collected: size and reproductive effort of 8 focal species
Samples or specimens collected: NA
Products: This work is part of Drake’s Ph.D research. He will be wrapping up this year, so stay tuned!
Land managers seek to introduce hemiparasitic plants (plants that steal nutrients from other neighboring plant species but still produce some of their own sugars through their own photosynthesis pathways) to restorations because hemiparasitic plants are associated with higher quality prairie. However, whether or not hemiparasitic plants are the cause of the prairie being high quality or the product of the prairie being high quality is unclear. Therefore, in 2020 we experimentally introduced 72 Pedicularis canadensis and Comandra umbellata into the restoration plot, experimental plot 1.
In 2023, we replanted 40 Pedicularis canadensis that haven’t been seen since they were initially planted in experimental plot 1. Additionally, we took our annual measurements of Liatris ligulistylis and Solidago speciosa size and reproductive effort, as well as counts of seedlings present. This year was the first year since 2019 that we did not harvest aboveground biomass around our 216 total planting locations in experimental plot 1.
Liatrisligulistylis in an experimental plot. Solidago speciosa in an experimental plot.
Start year: 2019
Location: experimental plot 1
Overlaps with: pedicularis effects on host plants in p10
Data collected: size, reproductive effort, and seedling count in Liatris ligulistylis and Solidago speciosa
Samples or specimens collected: NA! This year we did not collect biomass
Products: This work is part of Drake’s Ph.D research. He will be wrapping up this year, so stay tuned!
Ants are an integral part of ecosystems, playing a role in seed dispersal, detritus removal, pest predation, and nutrient cycling. Because ants nest in the ground, they are particularly susceptible to any process that disturbs the earth and can be heavily impacted by land use practices and management decisions. Diane Roeder, at Augustana University, designed this survey to quantify ant species diversity in remnant and restored prairie patches in western MN. These sites are primarily managed by fire, a type of disturbance that has been hypothesized to impact ant species differently via mortality and/or changes in habitat structure. During the summer, Diane and members of team Echinacea sampled 45 prairie sites (30 remnant, 15 restored), deploying a total of 415 pitfall traps. Diane and her colleagues are in the process of sorting ants from other ground-dwelling invertebrates captured by the traps and will identify specimens to compare abundance, species richness, and community composition from sites under different management regimes. In addition to measuring diversity, They also deployed sentinel prey items to determine whether arthropod communities in these areas remove prey at different rates as a measure of ecosystem services provided by predatory arthropods. To do this, they set out small cages containing moth eggs and recorded the number of eggs removed. In the future, Diane hopes to compare the overall arthropod communities between these types of sites from multiple years of sampling.
Diane traveled all over our study area during her few days in Western Minnesota.
This pitfall trap was set up at a random bb point in our study area.
Start year: 2023
Location: Prairie remnants and restorations in Solem Township, MN.
This experiment was designed to quantify how well Echinaceaangustifolia populations are adapted to their local environments. In 2008, Amy Dykstra collected achenes from Echinacea populations in western South Dakota, central South Dakota, and Minnesota and then sowed seeds from all three sources into experimental plots near each collection site. Each year, Team Echinacea takes a demographic census at the western South Dakota and Minnesota plots; we abandoned the central South Dakota plot after it was inadvertently sprayed in 2009, killing all the Echinacea.
In 2023, we found a total of 119 basal plants and 8 flowering plants. All of the flowering plants observed in 2023 were in the western South Dakota sowing site. Only 2 plants in the Minnesota site have ever produced flowers. In contrast, 31 plants flowered in the western South Dakota site in 2022 alone. Mortality has been much higher in Minnesota than in western South Dakota; thus, the total number of plants at each sowing site is now about equal.
Surviving plants at GRNG (Grand River National Grassland in western South Dakota) and HL (Hegg Lake in Minnesota) in annual censuses from 2014-2023.
Start year: 2008
Location: Grand River National Grassland (Western South Dakota), Samuel H. Ordway Prairie (Central South Dakota), Staffanson Prairie Preserve (West Central Minnesota), and Hegg Lake WMA (West Central Minnesota).
Data collected: Plant fitness measurements (plant status, number of rosettes, number of leaves, and length of longest leaf)
Samples or specimens collected: Heads from all flowering plants; Amy stores the heads in her office at Bethel University.
Products:Dykstra, A. B. 2013. Seedling recruitment in fragmented populations of Echinacea angustifolia. Ph.D. Dissertation. University of Minnesota. PDF
You can read more about Dykstra’s local adaptation experiment and see a map of the seed source sites on the background page for this experiment.
