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Stipa is done (and so am I!)

Just a quick note to say that Stipa planting was completed by lunch on Wednesday. We were able to plant the vast majority of seeds where they were randomly assigned in the CG, 10 cm north of existing Echinacea positions on the whole meter throughout the garden. I think only two seeds were not planted at all; one was lost off the board and one was missing its awn and ID sticker. About a dozen seeds had to be assigned new locations, either because there was a hole or a rock in the way or because the plant/staple/can’t find flag that we were measuring from could not be located. Not bad! To assign new random positions, I simply grabbed an ID sticker from the sheet of leftover, unused positions, removed the old sticker, stuck the new and marched around the garden planting the last of my legacy to the Echinacea project. The final task will be to replace the can’t find flags that are associated with a Stipa seed with short pins. Hopefully, this will up the chances of finding seedlings next summer. To this end, we also put a toothpick 1 cm north of every seed. Best of luck to the crew put to this test!
Also, sadly, my time with Team Echinacea is coming to a close. I have accepted an offer for an AAAS Science and Technology Policy Fellowship to work at the Environmental Protection Agency in Washington, DC starting September 1st (gasp, so soon!). I will be working in their environmental risk assessment group, spending MUCH more time at a desk and much less time running around in the field. I hope that my knowledge and skills as a scientist can do some good in the policy world. Thanks for a wonderful year, and a particularly wonderful summer. Stay in touch.

Searching Caroline’s Hegg Lake Plot

We searched for seedlings in Caroline’s Hegg Lake plot today. Old and new seedlings were found. Here are the data:
Nextgenresc-19 Aug 09data.xls

sites and ID’s pollinator comp 09

Howdy gang!
Here’s the list of tag ID’s and corresponding letters at the sites used this summer. We flagged different plants on 7/13 and 7/6 and used the same plants for observations on 7/21 and 7/23. For some reason I can’t find the list of flagged plants for 7/13, so it would be great if someone could check on the Hjelm house computer for that info. It may or may not be in the folder for this experiment. I’m sure I compiled that info from the visor memos, but I don’t have the file on my computer.
ech flagged plants and tags.doc
We recorded the tag ID’s during FNC so we could go back and check to make sure we had recorded the right number, but we never made the check.
Here’s the file that lists whether the vial had a bee, fly, bfly, or beetle in it:
ECH poll obs ALL.xls
Here’s the FNC Data in an excel file:
ECH FNC.xls

I hope everything’s going well in MN! It sounds like lots of progress has been made since I left. I thought my poster presentation went pretty well back in Chi-town. The final version of it is in a previous flog post. Thanks again to everyone…I certainly could not have done this without all of your help. I hope the field season ends well. Keep in touch. Oh and here’s an interesting paper I came across recently: brown bj loosestrife comp.pdf
And I really like this picture Daniel took:
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And here’s Echinacea taking center stage at CBG:
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Seedling re-finds

Here is a draft version of a protocol for seedling re-finds in the prairie remnants. Please read and critique. Protocol for seedling refinds 2009.docx

Diedre and Jake’s posters

I wanted to share Diedre and Jake’s REU posters with everyone…they both did a great job!
Poster-DRfinal.pdf
JJF Poster-final.pdf

Pollinating Cirsium

Here’s what needs to be done tomorrow, around 10am, for pollinating Cirsium altissimum at Hegg Lake.

I will provide a clipboard with a data sheet, map, and pollinating tools. Plant numbers are on flags to the south of the plants. Plant 9-7 has a yellow twist-tied head that is flowering right now. It will need to be selfed. Yellow tt heads on plants 9-16,-6, and -8 may be flowering tomorrow. If so, they also need to be selfed. Plant 9-19 had two bagged yellow heads. One is done flowering, the other may be flowering tomorrow. If the second one is flowering, it should be selfed. 9-26 with a red tt may be flowering. If so, it needs to be crossed. Cross pollen can be obtained from 9-14, which has a white tt and is blooming now, or 9-21, which may be flowering tomorrow.

To pollinate the heads, use a q-tip provided. For selfing, just rub the q-tip over the anthers to collect the pollen, then brush the q-tip on the stigmas. The pollen is very sticky and will easily stick to the q-tip. For crossing, rub a q-tip on the anthers of a pollen donor (white tt). Place in a labeled glass vial, transport to the head to be crossed, and rub the stigmas with the q-tip. Be sure to write down which plant was used as a pollen donor.

software for counting seeds in images

We have been very happy using ImageJ to count Echinacea seeds. ImageJ is free, open-source, public domain software. It runs on any platform.

We have also used ImageTool. This program is free and runs on Windows only.

Histogram of pollen sizes

Here’s a histogram of pollen sizes (~30 grains per species) from 3 individual plants of Coreopsis palmata, Echinacea angustifolia, and Heliopsis helianthoides.

gregPollenDataSet.png

Greg outlined the methods taking the measurements here. Greg, what software program did you use?

Mimi’s Last Day- long ago, but not forgotten!

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You WISH you were eating these cakes!!!!!!
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We miss you, Mimi!

You too, Greg!

pollen storage data

Here is a file with the pollen storage data (excluding Stuart’s data on the 48 hour style persistence).

pollen storage data sheet.xls

We pollinated 3 plants in the common garden that were still flowering. Each plant had 3 treatments of stored Echinacea pollen; ambient temperature, frozen, and refrigerated. The frozen and refrigerated pollen caused shriveling, the ambient temperature treatment did not.