During late summer 2021, we began collecting data in remnant patches of prairie to quantify fire effects on the reproduction of Big bluestem (Andropogon gerardii). We visited 376 random points established during the summer across 27 remnant patches and counted the number of flowering Andropogon culms rooted within 1 m of the random point. Across the 376 plots, we counted 1450 culms. The highest density we observed was 163 culms in one plot at KJs. We also collected seed heads from all culms within 1 m of the random points to x-ray and quantify seed set.
Jared searching in vain for Andropogon culms at Staffanson.
Start year: 2021
Location: Patches of remnant prairie in and around Solem Township, MN
Data collected: ~Dropbox/burnRems/remAndro/fieldData2021/remAndro2021DataVerified.csv
Samples or specimens collected: Seed heads collected during summer 2021 have been dried and currently reside in Jared’s office. These samples will be cleaned, processed, and x-rayed to quantify seed set.
Products: Stay tuned!
You can read more about the Andropogon fire and flowering in remnants experiment, as well as links to prior flog entries about this experiment, on the background page for this experiment.
In summer 2021, Team Echinacea searched for and mapped 708 flowering Prairie lily (Lilium philadelphicum) plants across 6 patches of remnant prairie in our study area in western Minnesota. Unlike many prairie species that flower vigorously after fire, these self-incompatible lilies flower two years after dormant season fires. We are investigating how fire influences lily flowering density and reproduction. We harvested seed pods from 80 individuals across 5 patches to quantify seed set.
Start year: 2021
Location: Remnant patches of prairie in and around Solem Township, MN
Overlaps with: NA
Data collected: demographic data: ~aiisummer2021/otherSpp/Lilium_philadelphicum/output/lilium2021Data.csv
Samples or specimens collected: Pods/seed collected during summer 2021 currently reside in Jared’s office. These seeds need to be inventoried, cleaned, counted, and scored for seed set.
Products: Stay tuned!
You can read more about the Lilium fire and flowering project, as well as links to prior flog entries about this experiment, on the background page for this experiment.
To examine the role flowering phenology plays in the reproduction of Echinacea angustifolia, Jennifer Ison planted this plot in 2006 with 3,961 individuals selected for extreme (early or late) flowering timing, or phenology. Using the phenological data collected this summer, we explore how flowering phenology influences reproductive fitness and estimate the heritability of flowering time in E. angustifolia.
In summer 2021, we visited 2,010 positions of the 3,961 positions originally planted. We measured 1,591 living plants, of which 681 were flowering, with a total of 1,283 flowering heads. In the fall, we harvested ~615 heads from exPt02. We do not have an exact number of heads harvested from exPt02 yet because we have not had time to complete head inventory. The large difference between the number of heads and the number harvested has to do with high levels of seed predation, mainly by ground squirrels.
Measuring p2 involved 9 different people working a total of 4295 minutes (71.58 hours) on 4 separate days.
Physical specimens: We harvested approximately 615 heads from exPt 2. Many heads were harvested by ground squirrels, so our number of heads does not match the number harvested. We brought the harvested heads back to the lab, where we will count fruits and assess seed set for each head.
Data collected: We collected data on each position planted. We recorded whether the plant was alive; if it was alive, whether it was flowering or basal. Basal plants had information about number of basal rosettes, number of basal leaves, and length of longest leaf. For flowering plants, we collected rosette counts and the height of each flowering head. When harvesting, we collected samples of loose achenes and recorded where they were from. Next spring, we will search for seedlings at these positions to ensure that there are not any rouge plants.
Products: Will and Jennifer are working on a manuscript using the flowering phenology data from this plot.
You can find more information about the heritability of flowering time and links to previous flog posts at the background page for the experiment.
In 2021, we collected data on the timing of flowering for 1692 flowering plants (2292 flowering heads) in 32 remnant populations. The plants ranged from having 1 to 18 flowering heads. The earliest bloomers initiated flowering on June 18, and the latest head initiated flowering on August 1. We identify each plant with a numbered tag affixed to the base and give each head a colored twist tie, so that each head has a unique tag/twist-tie combination, or “head ID”, under which we store all phenology data. We monitor the flowering status of all flowering plants in the remnants, visiting at least once every three days (usually every two days) until all heads are done flowering to obtain start and end dates of flowering.
Linking this detailed phenology data with information about seed production will help us understand how the timing of reproduction influences pollination and reproductive fitness. Additionally, we are excited to investigate whether fire synchronizes flowering in remnant populations. Eight of the populations in which we collected phenology data were burned during spring 2021.
Stylishly counting rows of styles while learning how to collect phenology data in remnants.
Start year: 1996
Location: Roadsides, railroad rights of way, and nature preserves in and around Solem Township, MN
Data/materials collected: We managed the data in the R project ‘aiisummer2021′ and will add the records to the database of previous years’ remnant phenology records, which is located here: https://echinaceaproject.org/datasets/remnant-phen/. The 2021 phenology data set needs to be cleaned and prepared for integration with phenology data from previous years and is still located in the aiisummer2021 repo.
Products: Stay tuned!
You can find more information about phenology in the remnants and links to previous flog posts regarding this experiment at the background page for the experiment.
Echinacea pallida flowering phenology:Echinacea pallida is a species of Echinacea that is not native to Minnesota. It was mistakenly introduced to our study area during a restoration of Hegg Lake WMA. Since 2011, Team Echinacea has visited the pallida restoration, taken flowering phenology, and collected demography on the non-native. We have decapitated all flowering E. pallida each year to avoid cross-pollination with the local Echinacea angustifolia. Each year, we record the number of heads on each plant and the number of rosettes. We also get precise gps coordinates of all plants and then chop the flowering heads off! This year, we cut E. pallida heads off on July 6th and 8th. We shot gps points as they were found; in the fall, we revisited the plants and did not find any stragglers.
Overall, we found and shot 143 flowering E. pallida plants, and 433 heads in total, averaging 3.02 heads per plant. The average rosette count was 5, the maximum was 27 rosettes — absolutely massive!! When recording data on E. pallida, we forgot that we needed phenology data, so the data from the 6th does not have any phen at all, and the data from the 8th is in the demo form in notes as a string. We do not have very accurate data on phenology of E. pallida this year, but our estimated first day flowering is June 22nd.
Pallida demo/cut/surv involved 7 different people working a total of 1170 minutes (19.5 hours) on 3 separate days.
Location: Hegg Lake WMA Start year: 2011
You can find more information about E. pallida flowering phenology and previous flog posts on the background page for the experiment.
exPt6: Experimental plot 6 was the first E. angustifolia x E. pallida hybrid plot planted by Team Echinacea. A total of 66 Echinacea hybrids were originally planted; all have E. angustifolia dams and E. pallida sires. In 2021, we visited 31 positions and found 15 living plants. No plants have flowered in this plot yet.
Location: near exPt8 Start year: Crossing in 2011, planting in 2012
You can find more information about experimental plot 6 and previous flog posts about it on the background page for the experiment.
exPt7: Planted in 2013, experimental plot #7 was the second E. pallida x E. angustifolia plot. It contains conspecific crosses of each species as well as reciprocal hybrids. There were 294 plants planted. This summer, we visited 176, and of these plants, only 136 plants were still alive. There were 13 flowering plants this year! This is the most flowering plants that this plot has produced. These 26 flowering plants produced 26 heads. We have not yet used the pedigree data to see what number of these plants are hybrids or not.
Location: Hegg Lake WMA Start year: Crossing in 2012, planting in 2013
You can find more information about experimental plot 7 and previous flog posts about it on the background page for the experiment.
exPt9: Experimental plot 9 is a hybrid plot, but, unlike the other two hybrid plots, we do not have a perfect pedigree of the plants. That is because the E. angustifolia and E. pallida maternal plants used to generate seedlings for exPt9 were open-pollinated. We need to do paternity analysis to find the true hybrid nature of these crosses (assuming there are any hybrids). There were originally 745 seedlings planted in exPt9. We found 261 living plants in 2021, 20 of which were flowering, with 42 heads! There were 138 plants that we searched for but could not find.
Location: Hegg Lake WMA Start year: 2014
You can find out more information about experimental plot 9 and flog posts mentioning the experiment on the background page for the experiment.
Measuring p6/7/9 involved 8 different people working a total of 1380 minutes (23 hours) on 2 separate days.
Experimental plots 6, 7, and 9 all burned this year. The peak in number of flowering plants in both p7 and p9 this year is indicative of the effect fire can have on flowering in Echinacea. In the past we have bagged heads in these plots but this year we did not.
Data collected for exp679: For all three plots, we collected rosette number, length of all leaves, and herbivory for each plant. We used visors to collect data electronically, and it is still being processed to be put into our SQL database.
Data collected for E. pallida demography and phenology: Demography data, head counts, rosette counts, gps points shot for each E. pallida. Find demo and phenology visor records in the aiisummer2021 repository. GPS coordinates can be found in demap. As mentioned above, all phenology data from July 8th can be found in demo. For more details, see aiiSummer2021/demo/pallidaPhen.R.
The crew poses after knocking out measuring half of p7 and p9
This Friday was day 5 of our externship and we had plenty of new experiences! We started the day by learning how to cleaning a different flowering prairie plant, liatris, or rough blazing star, with Wyatt. Compared to echinacea, achenes of liatris are much easier to be separated from the chaff and the receptacle. However, the fruits of the liatris are spread by wind. So the achenes all have fluffs attached to them which is a little challenging to separate sometimes.
Cleaning liatris!
Then we participated in the echinacea project’s weekly lab meeting. We read the draft of a paper Lea had been working on and listened to lab members discussing it. It was really fun and inspiring to see the process of scientific writing and experience the lab as such a collaborative space. I learned a lot about the behind the scene processes in doing science such as designing experiments, deciding on certain sets of data to use for a given topic and different ways to present data.
Right after the lab meeting, we listened to speed talks given by ecologists working in different departments of the Garden including aquatics, remnant forests and some other natural areas. I learned a lot about the history of the Garden as well as challenges and successes in managing different ecosystems within the Garden. I was really surprised when I learned how much work went into maintaining the lake area and the shores of lakes – planting, reinforcing, cleaning algae and other unwanted aquatic plants and so on. It was a great experience listening to talks by ecologists doing hands-on restoration and conservation work here in the Garden.
We also learned the ABT format of presenting a project and all three of us tried to come up with one for our projects here. We also decided on what we’ll be doing for projects. Cassie would be looking at density and seed set in liatris, Caitlin fire and seed set in echinacea, and I would be looking at number of flower heads and seed set in liatris. We planned to do some cleaning, rechecking, scanning, counting and X-raying to get part of the data we need and then do some data analysis. With this in mind, we were much more motivated to do some more cleaning and rechecking and dive into the second week of our externship!
Caitlin (left) and Cassie (right) focused on rechecking echinacea
As the first week of our externship is coming to a close, we’re learning more about the different procedures and tools used in the lab. After finishing up our rechecking and organizing of the 2020 harvest yesterday, we began scanning today so that we can eventually move on to the next step and count achenes. While we scanned, we also took turns continuing the daunting task of rechecking the cleaned 2021 harvest. Here’s Wanying and Caitlin working on rechecking while I scanned:
As fun as scanning and rechecking is, the most exciting part of my day was easily getting to watch a prescribed burn on some of the restored prairie. While the fire was pretty slow and patchy for the most part, it picked up speed during the tall grass sections and was pretty cool to watch overall. Getting to actually see a prescribed burn in practice after learning about it and its effects in the lab was really interesting. It was also a pretty valuable experience in that we got to watch and learn about how prescribed burns are done and the different tasks and precautions that go into the process.
Some of the patchy burn at its early stages.
After watching the burn and doing some more rechecking and scanning, we had a meeting with the lab team to brainstorm for the independent projects we’ll be working on for the last two weeks of our externship. We talked about different possible project ideas and questions we could investigate, but struggled some with the feasibility of accomplishing our goals in just two short weeks. However, after some more brainstorming and reevaluating, we were able to come up with some exciting ecological questions to investigate in the lab for the next couple of weeks!
Overall, this week has brought lots of new knowledge about the lab processes and the various projects they contribute to, as well as some great background and context for thinking about goals and ideas for the rest of the externship!
Today marked the third day of our Carleton externship! We started out early in the lab, cleaning echinacea heads with some admirable longstanding volunteers. Then we started rechecking the 2020 cleaned batches instead, and I felt like I had moved up in life.
Funnily enough, a couple of days ago I was timid to touch anything in the lab that I could possibly mess up, but now I just dive in and a few hours pass by like nothing. After we finished the 2020 rechecking (yay!), Wanying and Cassie started reordering the 2020 envelopes, which were assigned random labels in order to remove biases during analysis.
I, on the other hand, started rechecking the 2021 batch. I definitely realized how much I loved having the others’ help, as after today I am still left with this 2021 mountain of a box:
In addition to learning more about the research process, I enjoyed our meeting with Jared today, who prompted us to start thinking about what projects we want to pursue during the externship. Some ideas came up about working with the data at different stages, meeting with other employees at the garden, and proposing hypotheses that can be answered with the team’s available lab data.
The day ended with some great banana bread from Alex and a plan of more project group brainstorming the next day. My goals for tomorrow include both the project focus and the 2021 box. Tomorrow we also move along the process and start scanning the ordered achenes.
Overall, today gave me an appreciation for the people contributing to this project as well as excitement for how I can explore my curiosities using the research and guidance of this incredible team.
