In one of our most pristine tallgrass prairie habitats that remain in west central Minnesota, an accident was made in its management (not by Team Echinacea or members of The Echinacea Project). A Minnesota non-native Echinacea, Echinacea pallida, was seeded into the habitat. These non-native Echinacea flower at a similar time to our native Echinacea angustifolia and we suspected that they could cross-pollinate. To test this, an experimental cross was conducted where pollen from donors of both species E. angustifolia and E. pallida were applied to stigma of receptive Echinacea of both species (so we have four crosses: Ang x Ang, Ang x Pal, Pal x Pal, and Pal x Ang). These crosses produced some viable seed and the progeny were grown in an experimental garden. We monitored the progeny’s survivorship, size, and reproductive effort (once they began flowering in the 5th year). These data will allow us to answer questions about the fitness of hybrid Echinacea compared to non-hybrids.
I made an aster graphical model that I am going to use to test hypotheses about the fitness of hybrid Echinacea. The graphical model contains survivorship (represented as ld or “living during”), whether the plant flowered (Flowering), and a count of the number of heads produced by a flowering plant (HdCt). A noticeable feature of the graphical model is the absence of flowering in 2019, this was because none of the plants in the experiment flowered that year. In the previous year, the first plant flowered and it was the only plant to flower, interestingly it was a Pal x Pal cross.
The goal of this second data exploration was to determine the number of plants living in 2023 in each cross type and their plant status (e.g. basal, flowering, etc).
Table 1: Number of plants of each plant status given cross type in 2023. Interestingly, 0 Ang x Pal individuals flowered that year. Pal x Pal crosses flowered most abundantly.
Additionally, we wanted to know how many plants flowered each year given their cross types.
Table 2: Number of flowering individuals per year given their cross types. Interestingly, Pal x Pal were the earliest to flower and most abundantly flowering. Pal x Ang crosses flowered more than Ang x Pal crosses.
The goal of this initial data exploration was to determine questions and hypotheses that I will be exploring with aster models. To do this it is important to see what groundwork has been laid. Nicholas Goldsmith is the one who laid most of the groundwork on analysis for this project and it can be found here: https://conservancy.umn.edu/handle/11299/252544
Initial question: How does pedigree effect reproductive effort (i.e. flowering)?
This is of interest because 61 plants in experiment 7 have flowered since 2018 (this may not be 61 unique flowering plants but instead 61 unique instances of flowering across those years [2018 – 2023]).
Attached is a csv containing the coordinates for parasitic plantings in exPt01. ID is a unique ID to a Row x Position combination, Row is the row in exPt01 (starts at row 13 and increases by 5), Position is the position in exPt01 (starts at 861.85 and increases by 5), Treatment is what was placed there (seeds, Comandra, Pedicularis, or C + P which is Comandra and Pedicularis), siteOne is where the first plant planted came from and siteTwo is where Pedicularis came from if Comandra and Pedicularis were both planted.
To experimentally test hypotheses about how much Pedicularis canadensis, a native hemiparasite, affects the demographic rates (survival, growth, and reproduction) of other species, we planted plugs of P. canadensis in the center of a circle (with a radius of 20 cm) that contains 8 species. These eight common native prairie plant species are Echinacea angustifolia, Liatris ligulistylis, Solidago speciosa, Dalea purpurea, Pediomelum argophyllum, Sporobolus heterolepis, Koeleria macrantha, and Hesperostipa spartea. For all but Echinacea, seed was collected last year from local sources. Echinacea is the focal species of other experiments and had been planted previously. Echinacea plants served as a reference point when establishing our circles and were always directly west of P.canadensis. Circles are planted in 6 rows that were randomly selected from within the existing experimental plot 10. Rows 315, 436, 443, 643, 656, and 785 were selected. Rows contain 11 circles each, starting at 1m and going to 11m, evenly distributed 1m apart.
All circles were planted on July 29th, 2021.Plants were planted as plugs. Plugs were grown by Chicago Botanic Garden production staff before being transported to Minnesota and transplanted. Pedicularis served as the treatment and had 3 factor levels (0, 1, or 2 Pedicularis plants). Treatments were randomly assigned to circles and Pedicularis were planted in the center of each circle between August 9th and 13th, 2021. Plants in the circles were measured between August 16th and 20th, 2021.Traits measured were size and reproductive status.
Over the last two years I designed and planted an experiment in an already established prairie restoration (exPt01) to test hypotheses about the effects of parasite inclusion in restorations. This experimental planting of hemiparasites has three factors (Comandra umbellata, Pedicularis canadensis, and soil plugs), each with two levels (presence or absence), but three factor-level combinations are impossible because the presence of parasites is confounded with presence of soil. This translates to me having 216 row x position combinations in which I randomly assigned Comandra umbellata, Pedicularis canadensis, and soil plugs. However, roots trap soil and therefore soil is always carried in with parasites, the two treatments are confounded and so we used soil transplants to account for this.
In June, I went out and assessed the realized design of my experimental planting of hemiparasites. I assessed presence or absence of Comandra umbellata and Pedicularis canadensis at each of my 216 row x position combinations. I found only one Comandra but I found 30/72 or 42% of all Pedicularis.
In late August through early September, I planted 1 plug of Liatris ligulistylis and one plug of Solidago speciosa at each of my 216 locations. These plants were then measured. I added these plugs to serve as response variables to my three-factored experiment.
Last year, at all 216 locations I distributed seeds from 32 native plant species. In September, I went out and assessed seedlings present. I recorded the number and photographed the seedling to identify later in the laboratory.
In late October I intend to harvest 216 strips (0.1m x 1.0m) of dried biomass (1/6th of the dried biomass) from my 216 locations as I have also done in the last 2 years.
Start year: 2019
Location: Douglas County, Minnesota; exPt 1
Overlaps with: Experimental plot management, Hesperostipa common garden experiment
Materials collected: 216 .1 x 1m strips of dried biomass are stored at the Chicago Botanic Garden.
Stuart and Drake on November 24th, 2020 discussed how Drake should go about entering the data he collected on his circles, apply named “Drake’s circles for the possible intedended purposes of burning with fire and planting with parasites” or “demcirc” for short.
Drake went to the following sites and set up 10 randomly placed (to the best of his ability using his cellphone, laptop, and paper maps) circles: South of Golf Course, East Riley/Riley (10 total across both), Woody’s, Leuffler’s Corner East, Leuffler’s Corner West, Hill across from Leuffler’s Corner West, RRX, NRRX, In front of the Tower Rd Tower, Nice Island, The hill just east of Unity Drive on Hwy 27, Around Landfill North, Around Landfill South, Sap, TH, NNWLF, and KJs.
Additionally 30 circles each at: Aan, Eelr, Landfill, AgalinisRRX (NW Corner of Roland Lake RD and Hwy 55), and all-around Wennersborg Rd SW & HWY 55.
Each circle had a radius of ~0.4m and I recorded every species present within the circle and I recorded whether they were flowering (or had flowered at some point that year) or not.
Throughout the summer, I designed and collected materials to establish an experiment in experimental plot 1 to study parasites and their impact on the community of host plants they live in. Parasitic plants are plants which absorb nutrients from neighboring plants. Parasitism is an important part of nutrient cycling in many ecosystems and parasite scientists hypothesize it to be an important part of prairie ecosystem maintenance.
This experiment has three factors, each with two levels (presence or absence), but three factor-level combinations are impossible, because the presence of parasites is confounded with presence of soil. Which translates to me having 216 row x position combinations in which I randomly assigned Comandra umbellata, Pedicularis canadensis, and soil plugs. However, because roots trap soil and therefore soil is always carried in with parasites, the two are confounded and so we used soil transplants to account for this.
I developed this experiment to address questions about the impact native parasitic plants have on plant community members. In late October I harvested biomass from my pipp places to understand how species diversity and abundance change after planting parasites.
Start year: 2019
Location: Douglas County, Minnesota; exPt 1
Overlaps with: Experimental plot management, Hesperostipa common garden experiment
Materials collected: 216 .1 x 1m strips of dried biomass are stored at the Chicago Botanic Garden.
Data collected: Find data related to this project including the planting scheme in the cgdata repository in ~cgdata\summer2019\Hemiparasites (note/the key for HemiparaMap: C. umbellata = Blue, P. canadensis = Red, C + P = Purple, Soil plugs = Brown, Just seeds = Green).
Throughout the summer, I designed and collected
materials to establish an experiment in experimental plot 1 to study parasites
and their impact on the community of host plants they live in. Parasitic plants
are plants which absorb nutrients from neighboring plants. Parasitism is an important
part of nutrient cycling in many ecosystems and parasite scientists hypothesize
it to be an important part of prairie ecosystem maintenance.
This summer I collected seeds from five parasitic
plant species which are native to the prairie. I also collected the seeds of
over 100 species that can be commonly found in Douglas County, Minnesota and I
have begun experimental germination of them and will continue to do so in the
future. I developed a plan to plant Comandra and Pedicularis throughout exPt 1
and establish communities of 40 host species around them to address questions
about the impact native parasitic plants have on plant community members. In
late October I harvested biomass from the proposed parasite planting locations
to understand the species diversity and abundance present before planting.
Start year: 2019
Location: Douglas County, Minnesota; exPt 1
Overlaps with: Experimental plot management, Hesperostipa common garden
experiment
Materials collected: Parasitic plant seeds (Cuscuta
glomerata:18,000 across 6 individuals in 4 locations; Agalinis aspera: ~8,000
across 81 individuals in 3 locations; Agalinis tenuifolia: ~4,500 across
41 individuals in 1 location; Pedicularis canadensis: ~14,000 from 1
location; and Comandra umbellata: ~1,800 from 3 locations) and host
plant seeds (500+ seeds per host species, numbering approximately 100 species).
Seeds are stored at the Chicago Botanic Garden.
Additionally, 216 .1 x 1m strips of dried biomass are
stored at the Chicago Botanic Garden.
Data collected: Find data related to this project
including the proposed planting scheme in the cgdata repository in
~cgdata\summer2019\Hemiparasites
This week I spent my free time exploring the wet prairies of Douglas County, MN.
This is a picture of a small piece of wet prairie. Notice the high level of biodiversity! How many different species can you see? If you look closely, you’ll notice small pink flowers (it also helps if you zoom in). Those small pink flowers are the flowers of Agalinis tenuifolia which is the species I studied in my Master’s and is a parasitic plant that feeds off the roots of nearby plants!
Today I headed back to Chicago for my Master’s defense which is on Tuesday.
I met my new roommate Nigel.I have to drop off all my seeds at the Chicago Botanic Garden this week. I have used over 1500 coin envelopes on seed collections!
I need to get back to working on a grant, but first I want to show the world the first meme I ever made.
And take a moment also to appreciate that sometimes things just line up.
