This week, I have decided on my independent project for the rest of my internship! I will be working with Lilium philadelphicum, wood lily and investigating questions surrounding its pollination and reproduction. I felt more informed on the past research by the Echinacea Project after hearing presentations from Stuart and Jared. Jared further taught me the benefits of fire on prairie ecosystems. Prairie plants are fire dependent and thrive after burns. This is due to the natural landscape and indigenous traditions. It was very interesting to learn about the natural history of our region and how plants grew before impacts of modernization. Results from past research in the lab has shown benefits from prescribed burns on the reproduction of echinacea. This applied conservation method could potentially benefit other fire dependent prairie plants as well! I hope to observe patterns and variation of pollination success in L. philadelphicum. I am currently developing a hypothesis for this project. Later on I will analyze the data set collected by Jared over the summer.
We have already completed inventory of the data set and started on the cleaning process of L. philadelphicum. The fruits of L. philadelphicum can be seen in the picture below. It contains many seeds, some of which are large and dark, others small and lighter in color. We hope to find interesting information through the variation of these seeds. Stay tuned for next week, as I will be working on developing the protocol for cleaning, scanning and counting these seeds.
The fruit/pod of wood lily contains three separate chambers stacked with seeds.
This summer we harvested seed pods from 25 Green Milkweed (Asclepias viridiflora) plants in the study area. Green Milkweed is uncommon and seems to be declining in our study area. This species prefers similar habitat to Echinacea. Plants tend to be sporadically distributed across dry prairies on steep hillsides, sandy soils, and well-drained gravelly areas. Our experience has been that flowering plants often fail to produce seed. We rarely find more than a handful of plants that produce pods in a given year.
Flowering Green Milkweed with bumblebee visitor at Staffanson.
Green Milkweed with three pods at Landfill East.
After harvesting and drying seeds, Jared cleaned seed by removing their fluffy coma. Jared then counted all the seeds and randomly selected a minimum of 30 seeds for x-raying. X-rays revealed variation. Some ovules lacked an embryo , others had whole, intact embryos. Many ovlues fell somewhere in between. They contained embryos that were undersized, shriveled, or fragmented. There was no external evidence of seed predation. The proportion of full ovules ranged from 0 to 100 percent. We are not sure whether “partials” reflect resource limitation and seed abortion, a form of late-acting self-incompatibility, or something else entirely. We are doing some research to help us interpret the biology underlying these patterns.
X-ray image of Green Milkweed seeds. Note the variation in embryo size, shape, and integrity in the x-ray image.
Scanned image of the same Green Milkweed seeds.
After cleaning, counting, and classifying, Jared prepared a subset of Green Milkweed seed for germination. CBG’s production greenhouse will germinate and grow 392 milkweed seedlings representing 15 maternal lines. We will plant these seedlings in an area south of P8. Although these plants grow slowly, our hope is that they will be an excellent resource for investigating milkweed pollination in a couple years. We also hope to harvest seed from these plants and include Green Milkweed in our seed mixes for restoration!
In the first two weeks of being at the Echinacea Project Lab, I have become well acquainted with parts of the ACE protocol. I have done counting, cleaning, re-checking, randomizing, and scanning. In my first week, Alex showed me how to clean echinacea heads. During the cleaning process, we press the dried echinacea head into a glass pyrex tray. Usually, the achenes will fall out. However, i’ve gotten some really tough flower heads where the achenes were firmly attached to the bracts and other parts of the head. Alex taught me a good method to gently grab onto the achene with tweezers and wiggle them out of the head. After adapting that hack, things went much smoother. The contents of each head get separated into two envelopes. The achenes are stored in the achene folder and the bracts and dust are stored in the chaff folder. The achenes are ready to be scanned after a round of rechecking to ensure all achenes are accounted for!
I found it fascinating that there is so much variation in achenes between different echinacea heads. On the other hand, there are many similarities between achenes from the same flowering head. I also realized that handling achenes is a very delicate process because they have a tendency to fly away. I was very impressed with the organization system of all the achenes going through the ACE protocol. Different boxes and labels indicate which stage the achenes are currently at in a certain box.
Next week, I will be developing some questions for my own research project. I am excited to start my journey to uncover the secrets of echinacea!
Me cleaning an echinacea head! Picture taken by Alex.
I am part of the Class of 2025 at Carleton College and I plan to major in Environmental Studies.
Research Interests
In general, I am interested in helping to conserve natural prairie areas through networks and corridors so that they can become more resilient to climate changes. I am also curious about how to connect with stakeholders and landowners to make this happen, but also how to combine my interest in GIS with prairie restoration.
Statement
I am from Rochester, MN, but my home away from home is our cabin near Lanesboro, where I help to restore the surrounding native prairies, forests, and savannas. I also greatly enjoy hiking the bluffs there with my family and dogs.
Collecting seed with my dad at our cabin for Project Wingspan
It has been a hectic couple weeks for the Echinacea Project. Last week we braved fog, wind, and rain to wrap up the 2021 field season. Mia and Alex drove up from Chicago to help finish planting and pick up flags in the remnants.
On Thursday and Friday, Mia, Alex, and I sowed Echinacea seed in the 76 transects that comprise our seed addition experiment. This experiment will help us quantify the effects of fire and other environmental variables on seedling emergence and survival.
The planting went smoothly, we averaging approximately 2 minutes per transect. Prior to sowing seeds, we located transects and found nails designating the start and end of focal 1-m segments. We laid a meter stick on the ground stretching from start nail to end nail. We then spread seeds evenly between the 5 and 95 cm marks of the meter stick in line with the transect. Once empty, each seed envelope was given one last flick to dislodge any stubborn achenes and we gently tapped the meter stick against the ground to ensure any achenes that landed on blades of grass or other vegetation were not catapulted across the prairie when we picked up the meter stick.
Barring a shift in the weather that brings more favorable conditions for burning, Team Echinacea has moved indoors for the winter.
As part of the 2020 NSF grant to study fire effects on plant reproduction and population dynamics, we are implementing a seed addition experiment in numerous remnants. From previous studies, we know that fire can improve recruitment which is important for population growth. However, our previous observations of recruitment in remnants conflate the amount of seed entering the seed bank and the seedlings emerging from the seed bank. The goal of this seed addition experiment is to help us directly quantify the effects of fire on seedling emergence and early seedling fitness. We will use these data to parameterize demographic models for Echinacea.
For the seed addition experiment, we established 76 transects distributed across 32 prairie remnants with Echinacea. Transect locations were determined by generating an ordered list of random points (random integers corresponding with MN state plane coordinate system) within each remnant and selecting the first 2-4 random points that were located within ~5m of an adult Echinacea but avoided dense patches of flowering plants where we may have difficulty distinguishing experimental seedlings from natural recruits. Each transect originating at a random point is 4-m long and contains four 1-m segments. Most transects extend North from the random point but some extend East (in sites where North-South transects may span an entire ditch). One segment per transect was chosen at random to be planted in fall 2021 and one transect chosen at random to be planted during fall 2022. The study includes 9 sites burned during spring 2021 as well as 7 sites slated to burn during spring 2022.
Over the past week, I have been using a GPS unit to stake the transect locations. I marked the start and end point of each transect using a blue pin flag and installed nails at 1-m intervals using measuring tape (this will facilitate the use of meter sticks and/or measuring tapes in the field). We used 4 inch galvanized common nails. Pro tip: these nails were considerably less expensive at Fleet Farm than other vendors. I plan to finish setting up transects either this afternoon or tomorrow afternoon. Our goal is to begin sowing seeds later this week.
After a balmy stretch of weather (at least balmy for Minnesota in October), colder temperatures and shorter days have descended upon western MN. We experienced a killing frost Saturday (Oct 16) morning. Dwight and Jean reported that their tomatoes and other frost-sensitive garden plants are done for the year. I also awoke to frost this morning after a cold and rainy Wednesday (Oct 20).
During two of the next three nights, overnight lows are predicted to dip below 30 F. The forecast is also calling for a chance of snow (mixed with rain) Sunday morning. We had hoped for a dry, sunny stretch of weather this fall favorable for burning but the next week looks iffy.
Last week, we wrapped up the last of the fieldwork in Minnesota, although four Liatris plants are taking their sweet time and weren’t ready to harvest on Friday. The remaining members of Team Echinacea packed their bags and headed to the Chicago Botanic Garden, with the exception of Jared, who is staying to monitor the stubborn Liatris. Previously, I had never been to the Garden before, so it’s been a fun place to explore. I’ve also enjoyed the elaborate Halloween decorations in the neighborhood.
A familiar crime?Clowning around with the Grinch
This week at the Botanic Garden, we welcomed back Allen, our first volunteer since the beginning of the pandemic. It will be terrific to have some experienced volunteers to process the backlog of echinacea heads from the past several years.
At the lab, we’re also preparing for the seed addition experiment. Today, Wyatt trained us in on the seed blower, a contraption that separates light achenes from heavy ones. The heavy (rich) achenes should contain seeds, and we will next randomize the rich achenes for planting this fall. We need 12,800 seeds for the experiment, and after several trials with the seed blower, we estimate that we should have enough.
I had a blast being a part of Team Echinacea this summer. In my 14 weeks with the project, I spent my days in beautiful prairies, gained lots of sciency skills, made many friends (both people and plants) and enemies (mainly ground squirrels), and overall had a lovely experience.
Mapping the Liatris asperaI’m not even going to pretend that I was doing work here and not posing, thanks for the pic, AllieTrying to get all the angles on a butterflyIn action!
This flog post is to reminisce about all the fun I had this summer and to commemorate the fact that I will be staying on the team through the fall and for the foreseeable future! Because the Chicago Botanic Garden is so close to Northwestern, I’m able to travel there by bus to work in the lab. Better yet, Stuart was able to set up the position so that I would be able to receive work study funding from the school.
Hanging out with CollinsAnother little Liatris buddyAlex W. and I looking majestic, taken by professional photographer MiaTeam Andes looking flyMaybe the real Echinacea Project was the friends we made along the way… (many not pictured here)
Yesterday was my birthday, this is the second birthday that I have celebrated out here in Western Minnesota. The work day started with some sling (seedling re-finds), Alex and I did sling at Steven’s Approach and then we set off to Nessman. We quickly discovered that part of the site was mowed, and we had to go back to Hjelm to get the GPS to re-find the circles we needed to visit. We were driving away from Nessman at the corner of Dairy drive and 27 I saw something on the road. I asked Alex what it was, and she peeked out the passenger side window and said, “it’s a zucchini!” As we drove back to get the GPS, we contemplated whether we should rescue the zucchini or not. Once we saw the zucchini again, we knew we had to rescue it. After we finished at Nessman, we set of to procure our roadkill! We decided that it was most likely fell of a truck and then was run over. We scooped it up and removed the ant and millipede then buckled it into the back seat.
Finding the roadkill
We then set off to Staffanson to visit two more sling circles, the two circles are on complete opposite ends of the prairie preserve. Neither circle was fairly straight forward so after we finished the last circle Alex flopped down onto the ground, I quickly joined her, and we just laid there for 10 minutes staring up at the sky taking it all in. We eventually decided that we should probably head back for lunch, and after a bit of a hike back to the car we were shocked to see the zucchini since we had forgotten all about it.
The prairie nappers
After lunch I set of two experimental plot 1 to try and sort out some issues with the measuring data. Alex and Jared worked on sorting out some demo problems. It got up to 84 degrees Fahrenheit which might be the record high for September 28th (or at least it is based on my working memory).
For dinner Jared made spring rolls, he even had ripe avocados! Spring rolls have been a staple/highlight of the summer meals. After a yummy dinner Alex and I set out to turn our roadkill into cake. We quickly determined that the zucchini was in fact not zucchini but some other sort of squash. We decided out of impatience to not peel the squash. After making the cake and very patiently waiting for it to cool, we tasted it and it was surprisingly slightly crunchy. Overall it was a wonderful day, spent in a great place, with good friends, and good food.
