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Project status updates 2014

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Greetings from Chicago Botanic Garden! A new year is upon us and it is time to recap a busy 2014 summer. Over the next several weeks, we will be posting flog updates describing research projects from the summer and the status of the Echinacea Project’s long-term experiments. Stay tuned!

Click here to Browse all of the updates!

broadcast seeding

Dwight and Stuart broadcast native prairie seed in experimental plots p1 & p8 on Friday. At 34 °F (1°C) it was the warmest day in a month. It was also very windy –great for spreading seed! We broadcast Bouteloua curtipedula, Schizachyrium scoparium, Galium boreale, and Phlox pilosa directly on the snow. There wasn’t much snow and it was melting. We broadcast Lathyrus venosus in p1. We stored about half of each species, except L. venosus, in the Hjelm house to broadcast in the spring. (Hedging our bets.)

Project status update: The mating scene (remnant asynchrony, isolation, and incompatibility)

This project investigates the role of three fundamental constraints on mate availability – temporal asynchrony, spatial isolation, and reproductive incompatibility – in remnant Echinacea angustifolia populations. During the summer of 2014, we conducted two studies related to The Mating Scene project. In the first study, we mapped 991 Echinacea plants and monitored the phenology of 1360 flowering heads across 31 remnants to quantify spatial isolation and flowering asynchrony. At the end of the season, we harvested 193 flowering heads from 25 remnants to assess seed set. In the second study, we performed 364 pollen crosses to characterize patterns of reproductive incompatibility within 10 remnants. With the data collected in 2014 and subsequent years, we aim to elucidate how the interactions between temporal asynchrony, spatial isolation, and reproductive incompatibility influence reproductive fitness in fragmented prairie remnants.

site # of flowering plants # of flowering heads # of crosses # of heads harvested
1 aa 60 83 36 5
2 alf 63 78 6
3 btg 3 3 2
4 dog 1 2
5 eelr 33 17 28 5
6 eri 38 54 5
7 eth 9 46 5
8 gc 6 6 3
9 kj 7 8 5
10 lce 90 70 24 5
11 lcw 51 95 24 5
12 lfe 64 103 24 5
13 lfw 89 57 24 6
14 ngc 8 5
15 nnwlf 2 13 5
16 nrrx 20 25 5
17 nwlf 13 16 5
18 on27 92 96 42 5
19 ri 82 112 44 5
20 rrx 43 47 33 5
21 rrxdc 3 3 2
22 sap 29 13 5
23 sgc 8 243 5
24 SppE 92 81 41 42
25 SppW 51 38  44 42
26 th 8 9 5
27 tower 7 11 5
28 waa 4 8
29 wood 4 4
30 yohE 4 5
31 yohW 7 9

Start year: 2014

Location: Phenology in 31 total remnants, compatibility in 10 remnants

Products: The phenology and compatibility datasets need to be made readyR. The harvested flowering heads are being processed at the Chicago Botanic Garden.

Overlaps with: phenology in six remnants, fire and flowering at SPP

Project status update: Fire and recruitment of Echinacea angustifolia

fire

Description: The Echinacea Project’s recruitment experiment examines the germination and survival of Echinacea angustifolia seedlings in oldfields and restored grasslands under different fire regimes. In 2014, Team Echinacea searched for and measured Echinacea plants in 60 study plots spread across 10 experimental blocks. After data collection was complete, we mapped all newly flowering plants. The goal of this study is to identify the environmental factors that influence the recruitment of seedlings and the long-term fitness of Echinacea plants.

Start year: 2001

Location: 10 experimental blocks located in oldfields and restored grasslands

Products: Raw data taken on paper were entered into database and verified. Flowering plants with new tags were mapped and the spatial data is located in the 2014 SURV files. Flowering plants with old tags were not mapped in 2014.

Project status update: Pollen longevity

Description: During the summer of 2014, Will Reed designed and executed an experiment to measure the efficacy of various pollen storage methods including storing pollen at room temperature, in refrigeration, and freeze-drying pollen. Between July 14 and August 8, he collected pollen from 15 different plants and performed a total of 186 hand pollinations on 50 plants. These results will improve pollen storage practices and expand the capability to cross plants that flower asynchronously or potentially in different years.

Start year: 2014

Location: P1

Products: A dataset and detailed methods are located in Will Reed’s Dropbox folder. Dataset needs to be made readyR.

We harvested the heads Will used as pollen recipients. He could remove the achenes to see if his crosses produced viable seeds.

Project status update: Phenology and fitness in experimental plot 1

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Experimental plot 1 (P1) encompasses 11 different experiments originally planted with a total of 10673 Echinacea individuals. These experiments include long-term studies designed to compare the fitness of Echinacea from different remnant populations (“EA from remnants in P1”), examine the effects of inbreeding on plant fitness (“INB” and “INB2”), and explore other genetic properties of Echinacea such as trait heritability (“qGen”). In 2014, Team Echinacea measured plant traits for the 5409 Echinacea plants that remain alive and followed the daily phenology of 567 flowering heads. Echinacea began producing florets on July 1 and continued flowering in P1 until August 24. The data collected in 2014 will allow us to estimate the heritability of various traits and assess the lifetime fitness of plants from the numerous experiments.

Experiment Year planted # alive # flowering # planted
1 1996 1996 314 115 650
2 1997 1997 270 57 600
3 1998 1998 32 3 375
4 1999 1999 542 106 888
5 1999S 1999 297 37 418
6 SPP 2001 318 14 797
7 Inbreeding 2001 221 15 557
8 2001 2001 170 11 350
9 Monica 2003 2003 28 3 100
10 qGen 2003 2501 122 4468
11 INB2 2006 716 41 1470

Start year: 1996

Location: experimental plot 1

Products:

Overlaps with: aphid addition exclusion, Pamela’s functional traits, pollen longevity, pollen addition exclusion

Poster Award at BSA

Davis Blasini was awarded the best undergraduate student poster in 2014 by the Ecology Section of the Botanical Society of America: “Introduction of Echinacea pallida in the Prairies of Western Minnesota and its Possible Effects on Native Echinacea angustifolia” Co-author: Stuart Wagenius.

Congratulations, Davis!

Field guide to insect visitors of Echinacea angustifolia

Steph wrote a field guide years ago to help us identify insects that visit Echinacea heads during the summer in Minnesota. It still serves us remarkably well, but hasn’t been posted until now. Here it is!

 

 

An August afternoon at Hegg

As August draws to a close, we have been busy wrapping up phenology, making progress on demography, and harvesting Echinacea heads. While Claire, Elizabeth, and Stuart harvested heads at Hegg Lake, I mapped a patch of Echinacea angustifolia located a couple hundred meters west of P2. The patch centers around a small knoll ringed by poison ivy. I shot GPS points for 7 plants on the periphery of this Echinacea patch. Within the patch, I found 30 Echinacea flowering plants with a total of 37 heads. Most plants were located on the southwest slope of the knoll. Although I did not find any individuals on the north or east slopes, these hillsides should offer good Echinacea habitat.

In preparation for the fall burn at Hegg Lake, we set up a small project to monitor a patch of Hill’s Thistle (Cirsium hilli). We identified, shot GPS points, and measured the basal rosette diameter for 28 individuals. After mapping and measuring the thistles, we split the patch into two plots and mowed burn breaks around our plots. The north plot will not be burned in the fall. Like Echinacea, C. hillii inhabits dry prairies but Hill’s thistle is somewhat rare and is listed as a Species of Special Concern in Minnesota. Fire is thought to be necessary for maintaining the native plant communities of dry prairies but little is known about C. hillii responds to fire.

Pollinator Efficiency Information Celebration!

I’ve attached some goodies for everyone today, which are strategically placed at the end of this post. I am heading back to California on Tuesday and this will be my last flog post. I am go grateful to have been given this opportunity and I will miss lovely meals, Gretel’s chocolate cake, and all of the wonderful people I’ve met. I could list many other things that I will miss, but I suspect everyone is anxious to get to the sweeter section of this post.

AKA: EVERYTHING ANYONE COULD EVER WANT/NEED TO REPEAT THE POLLINATOR EFFICIENCY EXPERIMENT

My original proposal (with preliminary protocol):
Pollinator Efficiency Proposal 2014 .doc
Revised Procedure:
Pollinator Efficiency Procedure.doc
The observation datasheet we used in the field:
PolObDatasheet_2014.xls
Observation Data for P1:
PolObData_P1_2014_Final.xls
Observation Data for P2:
PolObData_P2_2014_Final.xls
Video ID Data for 2014 in P1:
P1_videoIDdatasheet_2014.xls
Video ID Data for 2014 in P2:
P2_videoIDdatasheet_2014.xls
Video ID Data for 2010, 2012, and 2013:
VideoIDdatasheet_2010-2013.xlsx
Metadata for Datasheets:
Metadata for videoIDdatasheet_2010-2013.doc

Metadata for PolObData_P2_2014_Final.doc

Metadata for PolObData_P1_2014_Final.doc

Metadata for P2_videoIDdatasheet_2014.doc

Metadata for P1_videoIDdatasheet_2014.doc

Dataset used for Analysis:
Taxon_Styles_Years.csv
R-File for Statistical Analysis (thanks in large part to Jennifer/Jared):
MLP_Pol_Eff_Analysis.R
My CBG Presentation:
Pol_Eff_Presentation_MLP_2014 (1).pdf
An Abstract (which could be sent to funders):
Maureen Page Pollinator Efficiency Abstract.doc

PLUS HERE IS A PICTURE OF A MONARCH!!!

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I hope everyone enjoys their summer and I’m excited to see what happens in the months and years to come!

Cheers!
Maureen Page.